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NEK2 F6.2 gRNA

Plasmid

#90795

Purpose

3rd generation lentiviral gRNA plasmid targeting human NEK2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

NEK2 (Guide Designation F6.2)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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NCAPD2 E7.2 gRNA

Plasmid

#90778

Purpose

3rd generation lentiviral gRNA plasmid targeting human NCAPD2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

NCAPD2 (Guide Designation E7.2)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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CCT5 C12.3 gRNA

Plasmid

#90581

Purpose

3rd generation lentiviral gRNA plasmid targeting human CCT5

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CCT5 (Guide Designation C12.3)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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CCT5 C11.3 gRNA

Plasmid

#90580

Purpose

3rd generation lentiviral gRNA plasmid targeting human CCT5

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CCT5 (Guide Designation C11.3)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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DYNLL1 A8.5 gRNA

Plasmid

#90661

Purpose

3rd generation lentiviral gRNA plasmid targeting human DYNLL1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

DYNLL1 (Guide Designation A8.5)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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DYNLL1 A7.5 gRNA

Plasmid

#90660

Purpose

3rd generation lentiviral gRNA plasmid targeting human DYNLL1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

DYNLL1 (Guide Designation A7.5)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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ODF2 F8.3 gRNA

Plasmid

#90817

Purpose

3rd generation lentiviral gRNA plasmid targeting human ODF2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

ODF2 (Guide Designation F8.3)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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TPX2 H1.2 gRNA

Plasmid

#90918

Purpose

3rd generation lentiviral gRNA plasmid targeting human TPX2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

TPX2 (Guide Designation H1.2)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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BUB1 A6.1 gRNA

Plasmid

#90553

Purpose

3rd generation lentiviral gRNA plasmid targeting human BUB1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

BUB1 (Guide Designation A6.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 17, 2017

Availability

Academic Institutions and Nonprofits only

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pMElmo2P1gfp

Plasmid

#90127

Purpose

Gateway Mid-entry clone containing promoter sequence specific for lmo2 driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

lmo2P1Egfp (lmo2 Zebrafish)

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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p5Edll4E1

Plasmid

#90120

Purpose

5' Gateway entry clone containing endothelial specific enhancer 1 sequence of dll4

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

dll4E1 (dll4 Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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p5Elmo2E1

Plasmid

#90118

Purpose

5' Gateway entry clone containing endothelial specific enhancer sequence of lmo2

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

lmo2E1 (lmo2 Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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p5Edusp5E1

Plasmid

#90122

Purpose

5' Gateway entry clone containing endothelial specific enhancer sequence of dusp5

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

dusp5E1 (dusp5 Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 16, 2017

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;dusp5P1Egfp

Plasmid

#90161

Purpose

contains dusp5 specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

dusp5P1Egfp (dusp5 Zebrafish)

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pMEdusp5P1gfp

Plasmid

#90126

Purpose

Gateway Mid-entry clone containing promoter sequence specific for dusp5 driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

dusp5P1Egfp (dusp5 Zebrafish)

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pMEdll4P1gfp

Plasmid

#90125

Purpose

Gateway Mid-entry clone containing promoter sequence specific for dll4 driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

dll4P1Egfp (dll4 Zebrafish)

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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p5Edll4E2

Plasmid

#90121

Purpose

5' Gateway entry clone containing endothelial specific enhancer 2 sequence of dll4

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

dll4E2 (dll4 Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pET151-Vps4(101-437) L151D

Plasmid

#87736

Purpose

Bacterial expression of a monomeric variant of the S. cerevisiae Vps4 AAA ATPase cassette

Depositor

Chris Hill

Article

Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

Insert

Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)

Use

Tags

6xHis-V5-TEV

Expression

Bacterial

Mutation

deleted amino acids 1-100, mutated Leu 151 to Asp

Promoter

T7

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-Hook3 Q147A, M151A, I154A

Plasmid

#74616

Purpose

Expresses a triple mutant truncation of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-239, Q147A, M151A, I154A (HOOK3 Human)

Use

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Mutation

Q147A, M151A, I154A

Promoter

T7

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a-Hook3 D102A, E108A

Plasmid

#74618

Purpose

Expresses a double mutant truncation of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-239, D102A, E108A (HOOK3 Human)

Use

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Mutation

D102A, E108A

Promoter

T7

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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