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pLenti_CMV:Flag-CeNLuc_SV40:PuroR

Plasmid

#183036

Purpose

Expresses the FLAG-tagged CeNLuc (Blue LumiFluor) in mammalian cells.

Depositor

Antonio Amelio

Article

Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

Insert

CeNLuc LumiFluor

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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KA1875_pGL4-UBCmin-luc2/Sox2_SRR2

Plasmid

#124198

Purpose

Luciferase reporter driven by the Sox2 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Sox2 enhancer

Use

Luciferase

Tags

Expression

Mutation

Promoter

UBCmin

Available since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pSL0747 (pDonor_L-MmeI)

Plasmid

#130647

Purpose

Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene. The mutated left end introduces an MmeI recognition site (used for Tn-seq). Total transposon size = 977 bp.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchCAST donor DNA (L*)

Use

CRISPR; Transposon

Tags

Expression

Bacterial

Mutation

TGTTGATG --> TGTTGGAG

Promoter

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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mcherry Beta 1

Plasmid

#36111

Depositor

Narasimhan Gautam

Article

Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

Insert

G protein beta subunit B1 (GNB1 Bovine)

Use

Tags

mcherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 3, 2012

Availability

Academic Institutions and Nonprofits only
pYPQ141-ZmUbi-RZ-Aac.4

Plasmid

#158406

Purpose

Express single gRNA with Aac.4 scaffold (with four MS2 binding sites) under ZmUbi promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Maize ubiquitin 1

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV4a-SIRT6-G60A-FLAG

Plasmid

#102323

Purpose

Human SIRT6 G60A with FLAG tag for mammalian expression

Depositor

Hening Lin

Article

Zhang et al Nat Chem Biol. 2016 Aug;12(8):614-20. do

Insert

SIRT6 (SIRT6 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed glycine 60 to alanine

Promoter

CMV

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pMH-SFB-RNF169

Plasmid

#99392

Purpose

Mammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged RNF169

Depositor

Michael Huen

Article

Chen et al J Biol Chem. 2012 Aug 10;287(33):27715-2

Insert

RNF169 (RNF169 Human)

Use

Tags

SFB (S protein-FLAG-Streptavidin binding peptide)

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pSL0746 (pDonor_R-MmeI)

Plasmid

#130646

Purpose

Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene. The mutated right end introduces an MmeI recognition site (used for Tn-seq). Total transposon size = 977 bp.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchCAST donor DNA (R*)

Use

CRISPR; Transposon

Tags

Expression

Bacterial

Mutation

TGTTGATA --> TGTTGGAA

Promoter

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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PhyB-mCherry-NLS

Plasmid

#51571

Purpose

Constitutively express nuclear localized PhyB in Saccharomyces cerevisiae

Depositor

Chao Tang

Article

Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

Insert

PhyB (PHYB Mustard Weed)

Use

Tags

NLS and mCherry

Expression

Yeast

Mutation

1-908aa only

Promoter

ADH1pr

Available since

Aug. 18, 2014

Availability

Academic Institutions and Nonprofits only

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PhyB-mCherry-Htb2

Plasmid

#51569

Purpose

Constitutively express nuclear localized PhyB in Saccharomyces cerevisiae

Depositor

Chao Tang

Article

Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

Insert

PhyB (PHYB Mustard Weed)

Use

Tags

HTB2 and mCherry

Expression

Yeast

Mutation

1-908aa only

Promoter

ADH1pr

Available since

Aug. 18, 2014

Availability

Academic Institutions and Nonprofits only

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HSC1-GiP

Plasmid

#58254

Purpose

This self-inactivated retroviral vector has an internal human EF1alpha promoter driving the expression of a bicistronic EGFP ires Puromycin cassette

Depositor

James Ellis

Article

Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1

Insert

Enhanced Green Fluorescence Protein, Puromycin resistance gene

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Human EF1-alpha

Available since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pJIT163-eAFID-3

Plasmid

#167360

Purpose

For inducing predictable deletions in protoplasts and wheat plants

Depositor

Caixia Gao

Article

Wang et al Nat Biotechnol. 2020 Dec;38(12):1460-146

Insert

A3Bctd-Cas9-UDG-T2A-AP lyase

Use

Tags

Expression

Plant

Mutation

WT

Promoter

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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PhyB-mCherry-Spc72

Plasmid

#51582

Purpose

Constitutively express spindle pole body localized PhyB in Saccharomyces cerevisiae

Depositor

Chao Tang

Article

Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

Insert

PhyB (PHYB Mustard Weed)

Use

Tags

Spc72 and mCherry

Expression

Yeast

Mutation

1-908aa only

Promoter

ADH1pr

Available since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pGL3-Basic-hCYP26A1P-E4-luciferase

Plasmid

#135592

Purpose

Short form DNA fragment of human CYP26A1gene promoter (E4) in pGL3-Basic-luc vector

Depositor

Catharine Ross

Article

Zhang et al Gene. 2010 Sep 15;464(1-2):32-43. doi: 1

Insert

Short form promoter of human CYP26A1 gene (E4)

Use

Tags

Expression

Mutation

Promoter

Human CYP26A1 promoter (Short Form, E4)

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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KA1868_pGL4-UBCmin-luc2/Utf1-A'

Plasmid

#124194

Purpose

Luciferase reporter driven by the Utf1 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Utf1 enhancer

Use

Luciferase

Tags

Expression

Mutation

Promoter

UBCmin

Available since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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PhyB-mCherry-PEX3

Plasmid

#51572

Purpose

Constitutively express peroxisome localized PhyB in Saccharomyces cerevisiae

Depositor

Chao Tang

Article

Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

Insert

PhyB (PHYB Mustard Weed)

Use

Tags

PEX3 and mCherry

Expression

Yeast

Mutation

1-908aa only

Promoter

ADH1pr

Available since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pH-AFID-3

Plasmid

#167361

Purpose

For inducing predictable deletions in rice plants

Depositor

Caixia Gao

Article

Wang et al Nat Biotechnol. 2020 Dec;38(12):1460-146

Insert

A3A-Cas9-UDG-T2A-AP lyase

Use

Tags

Expression

Plant

Mutation

WT

Promoter

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pScalps eSpCas9_Zsgreen gRNA scaffold

Plasmid

#188449

Purpose

Expression of eSpCas9 tagged with Zsgreen

Depositor

Ajit Chande

Article

Mishra et al Mol Ther Nucleic Acids. 2022 Mar 8;28:20

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

EF1-alpha

Available since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pNL(CMV)TK2ALuc2/CMV/WPREdU3

Plasmid

#163965

Purpose

Co-expression of HSV-1 TK and firefly luciferase in mammalian cells

Depositor

Jakob Reiser

Article

Argaw et al Mol Ther Methods Clin Dev. 2021 Apr 24;2

Insert

HSV-1 TK and FLuc

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCI-MEG3b

Plasmid

#59421

Purpose

Expresses lncRNA MEG3 isoform b in mammalian cells.

Depositor

Anne Klibanski

Article

Zhou et al J Biol Chem. 2007 Aug 24;282(34):24731-4

Insert

MEG3b (MEG3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only

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