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Plasmid#91327PurposeProtein expression and purification of human SH3 domain construct MIA3-1/1DepositorInsertMIA3-1/1 (MIA3 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTYB11-PTBP1-RRM2CCtoSS
Plasmid#89154Purposerecombinant expression of PTBP1-RRM2 C250S,C251S in e.coliDepositorInsertPolypyrimidine Tract Binding Protein 1 RNA Recognition Motif 2 mutant C250S, C251S (PTBP1 Human)
UseTagsIntein and chitin binding domainExpressionBacterialMutationmutations: Cys 250 to Ser, Cys 251 to SerPromoterT7Available sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pD10AH840AhCas9 (GB1041)
Plasmid#68207PurposeProvides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid partDepositorInsertCas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removed; human codon optimis…PromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST27-GST-ATXN1 FL(84Q)-S776A
Plasmid#21758DepositorInsertAtaxin-1 (ATXN1 Human)
UseTagsGSTExpressionMammalianMutationinsert contains a stretch of 84 Q(Glutamines) and…PromoterCMVAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDEST27-GST-ATXN1 FL(30Q)-S776A
Plasmid#21756DepositorInsertAtaxin-1 (ATXN1 Human)
UseTagsGSTExpressionMammalianMutationinsert contains a stretch of 32 amino acids (30 Q…PromoterCMVAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
mChF-AIP (TD)
Plasmid#61529PurposeExpression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry and AIP (with TD mutation)DepositorInsertFK506 binding protein 1A (FKBP1A Human)
UseTagsAIP with TD mutation (see comments) and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
mChF-AIP (TE)
Plasmid#61530PurposeExpression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry and AIP (with TE mutation)DepositorInsertFK506 binding protein 1A (FKBP1A Human)
UseTagsAIP with TE mutation (see comments) and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEMS1210
Plasmid#29107PurposeExpression of the eGFP reporter gene was not detected in the brain and eye.DepositorInsertPle238 (TRH Human)
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-NLSExpressionMutationPromoterAvailable sinceNov. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.BRWD3-V5H
Plasmid#48624PurposeExpresses Drosophila BRWD3 (with V5 and His tags at the C-terminus) in mammalian cellsDepositorInsertBRWD3 (BRWD3 Fly)
UseTagsV5 and His tagsExpressionMammalianMutationno mutations, stop was removed to fuse V5His at C…PromoterCMVAvailable sinceOct. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV4-HA-p31(comet)-S102A
Plasmid#221698PurposeMammalian expression of HA-tagged human p31(comet) (S102A)DepositorInsertMAD2BP1
UseTagsExpressionMammalianMutationS102APromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-RNF8-R42A
Plasmid#221702PurposeLentiviral expression of human RNF8 (R42A) in mammalian cellsDepositorInsertRNF8 (RNF8 Human)
UseLentiviralTagsExpressionMutationR42APromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-RNF8-C403S
Plasmid#221703PurposeLentiviral expression of human RNF8 (C403S) in mammalian cellsDepositorInsertRNF8 (RNF8 Human)
UseLentiviralTagsExpressionMutationC403SPromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-RNF8-S157A
Plasmid#221704PurposeLentiviral expression of human RNF8 (S157A) in mammalian cellsDepositorInsertRNF8 (RNF8 Human)
UseLentiviralTagsExpressionMutationS157APromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLAG-MAD2-RQ
Plasmid#221691PurposeMammalian expression of FLAG-tagged human MAD2 (R133E/Q134A)DepositorInsertMAD2
UseTagsExpressionMammalianMutationR133E, Q134APromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4.1-Myc-RNF8
Plasmid#221676PurposeMammalian expression of Myc-tagged human RNF8DepositorInsertRNF8 (RNF8 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDF-rM1PYK
Plasmid#220232PurposeInducible expression of rabbit muscle pyruvate kinase isoform 1DepositorInsertRabbit muscle pyruvate kinase (PKM )
UseTags6xHis-MBP-SUMOExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLAG-MAD2
Plasmid#221690PurposeMammalian expression of FLAG-tagged human MAD2DepositorInsertMAD2
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLAG-p31(comet)
Plasmid#221692PurposeMammalian expression of FLAG-tagged human p31(comet)DepositorInsertMAD2BP1
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLAG-p31(comet)-QF
Plasmid#221693PurposeMammalian expression of FLAG-tagged human p31(comet) (Q83A/F191A)DepositorInsertMAD2BP1
UseTagsExpressionMammalianMutationQ83A, F191APromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLAG-RNF8
Plasmid#221695PurposeMammalian expression of FLAG-tagged human RNF8DepositorInsertRNF8 (RNF8 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only