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Plasmid#126910PurposeTemplate for PCR amplification of ADE2 gRNA biobrick for targeting Saccharomyces cerevisiae ADE2 geneDepositorInsertADE2 gRNA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
SlugABEmax
Plasmid#163798PurposeExpresses ABEmax and nSlugCas9DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationSlugCas9 (D10A)PromoterAvailable sinceApril 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0508
Plasmid#91014PurposeModule A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9, Terminator: HSPDepositorInsertCsy4-P2A-AtCas9
UseCRISPRTagsExpressionMutationPromoterEC1.2Available sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFH2.97_PB-EPN1-MCP-T2A-eGFP-PGK-Blast
Plasmid#205542PurposeExpress EPN1-MCP-T2A-eGFP and blasticidin resistance markerDepositorInsertEPN1-MCP
UsePiggybacTagsExpressionMammalianMutationWTPromoterEF1Available sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMC-6-AtSpCas9-9
Plasmid#197778PurposePhytobrick (MoClo) Level 0 PartDepositorInsertAtSpCas9
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMC-1-AtU6-Y
Plasmid#197779PurposePhytobrick (MoClo) Level 0 PartDepositorInsertAtU6 promoter for sgRNA assembly
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMC-1-OsU6-X
Plasmid#197780PurposePhytobrick (MoClo) Level 0 PartDepositorInsertOsU6 promoter for sgRNA assembly
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMC-Z-SpCas9-gRNA-repeat-polyT-11
Plasmid#197781PurposePhytobrick (MoClo) Level 0 PartDepositorInsertgRNA repeat for U6 sgRNA assembly
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
p32Fn
Plasmid#158153PurposeConstruct conventional Cpf1 vectors that uses U3p and UBI10p to express single crRNA (or PTC/CA) and LbCpf1, respectively. For plant genome editing.DepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationRice codon optimizedPromoterAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mH6-Cas12i2
Plasmid#120883PurposeExpresses E. coli codon optimized Cas12i2 effector in the pET28a backbone.DepositorInsertCas12i2
UseCRISPRTagsmH6ExpressionBacterialMutationWTPromoterlacAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH2.102_PB-EPN11-MCP_I-T2A-eGFP-PGK-Blast
Plasmid#205547PurposeExpress EPN11-MCP-I-T2A-eGFP and blasticidin resistance markerDepositorInsertEPN11-MCP-I
UsePiggybacTagsExpressionMammalianMutationWTPromoterEF1Available sinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pC0.167
Plasmid#119636PurposeLevel 0 Part. Insertion siteDepositorInsert6803 NS2 Up Flank
UseSynthetic BiologyTagsExpressionMutationnon functional/large non-specific insertionPromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0.168
Plasmid#119635PurposeLevel 0 Part. Insertion siteDepositorInsert6803 NS2 Down Flank
UseSynthetic BiologyTagsExpressionMutationbp 630 A to TPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPHAGE2-Myc-mTZAP
Plasmid#87188Purposelentiviral stable expression of Myc-TZAP (mouse) in mouse embryonic stem cellsDepositorInsertTZAP (Zbtb48 Mouse)
UseLentiviralTagsMycExpressionMammalianMutationPromoterunknownAvailable sinceMarch 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSEM228 - [Pmlc-1 | mNeonGreen(2x NLS) | cbr-tbb-2 UTR]
Plasmid#159895PurposeFluorescent co-injection marker to identify extrachromosomal arrays in C. elegansDepositorInsertPmlc-1 | mNeonGreen(2x NLS) | cbr-tbb-2 UTR
UseTagsExpressionWormMutationPromoterAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFH2.103_PB-EPN11-MCP_p6-T2A-eGFP-PGK-Blast
Plasmid#205548PurposeExpress EPN11-MCP-p6-T2A-eGFP and blasticidin resistance markerDepositorInsertEPN11-MCP-p6
UsePiggybacTagsExpressionMammalianMutationWTPromoterEF1Available sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-mTUT7
Plasmid#60044PurposeMammalian expression of FLAG-tagged mouse TUT7DepositorInsertTUT7 (Zcchc6 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-dSa-VPR mini.
Plasmid#99686PurposeExpresses dSa Cas9 fused to optimized combination of truncated activation domains from EFS promoterDepositorArticleInsertdCas9
UseAAVTagsVPR miniExpressionMutationdead Cas9PromoterAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMC-6-Cre-GR-9
Plasmid#197723PurposePhytobrick (MoClo) Level 0 PartDepositorInsertCre recombinase with St-LS1 intron fused to glucocorticoid receptor
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDonor14-ShoHELIX-KanR (BO4)
Plasmid#181795PurposepDonor for ShoHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargoDepositorInsertKanR, R6K origin of replication
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only