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Plasmid#136843PurposeMammalian expression of the centrosomal protein Cep41 N-terminally fused to CLIP-tagDepositorInsertCLIP-Cep41 (CEP41 Human, Synthetic)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
PCRII apoEa
Plasmid#121900PurposePlasmid for making zebrafish apoEa in situ probeDepositorInsertApoEa (apoea Zebrafish)
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEBTetDBl-CLIP-Cep97
Plasmid#136850PurposeMammalian expression of the centrosomal protein Cep97 N-terminally fused to CLIP-tagDepositorInsertCLIP-Cep97 (CEP97 Human, Synthetic)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTetD-SNAP-Cep97
Plasmid#136801PurposeMammalian expression of the centrosomal protein Cep97 N-terminally fused to SNAP-tagDepositorInsertSNAP-Cep97 (CEP97 Human, Synthetic)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_PfeAR480A-Q482A
Plasmid#128946Purposeexpresses R480A Q482A mutant of PfeA in E. coliDepositorInsertferric enterobactin receptor
UseTagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-75), …PromoterT7Available sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_DOCK5-1/1-IS1/2
Plasmid#91303PurposeProtein expression and purification of human SH3 domain construct DOCK5-1/1-IS1/2DepositorInsertDOCK5-1/1-IS1/2 (DOCK5 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_DNMBP-6/6
Plasmid#91489PurposeProtein expression and purification of human SH3 domain construct DNMBP-6/6DepositorInsertDNMBP-6/6 (DNMBP Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_KALRN-1/4
Plasmid#91283PurposeProtein expression and purification of human SH3 domain construct KALRN-1/4DepositorInsertKALRN-1/4 (KALRN Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
AcrIIC1X* (AcrIIC1X-mCherry chimera)
Plasmid#128114PurposeExpresses AcrIIC1(N3F/D15Q/A48I) fused to mCherry; mediates potent inhibition of both, N. meningitidis as well as S. aureus Cas9DepositorInsertAcrX* (AcrIIC1 N3F/D15Q/A48I fused to mCherry)
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTKan-p35S::Csy4-pNOS::cogGFP (C132, JBEI-15898)
Plasmid#110145PurposeTransformation and expression of Csy4 and cogGFP proteins in plantsDepositorInsertCsy4 (ATCS Mustard Weed)
UseTagscogGFPExpressionBacterial and PlantMutationPromoterAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 p37 deltaUBX
Plasmid#113504PurposeBacterial expression of GST-tagged p37 lacking the UBX domainDepositorInsertp37 (Ubxn2b Mouse)
UseTagsGST-PreScission protease siteExpressionBacterialMutationdelta 251-331 (stop codon behind AA250)PromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX Arg-SH2
Plasmid#46405DepositorInsertArg/Abl2 (ABL2 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 151-257PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX chimerin-SH2
Plasmid#46415DepositorInsertchimerin (CHN1 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 44-147PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Dapp1-SH2
Plasmid#46422DepositorInsertDapp1 (DAPP1 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 25-151PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA2_LC_P298L_S285C
Plasmid#104469Purposeexpress His tagged P298L S285C hnRNPA2 LCDepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTagsHisExpressionBacterialMutationP298L S285CPromoterAvailable sinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAPM-D4 miR30-AGO1 ts3
Plasmid#115847PurposeAGO1 knockdownDepositorInsertAGO1 shRNA (AGO1 Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX Mist-SH2
Plasmid#46454DepositorInsertMist (Clnk Mouse)
UseTagsAviTag, GST, and PreScissionExpressionBacterialMutationcontains amino acids 300-435PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ITSN1-1/5-IS2/3
Plasmid#91318PurposeProtein expression and purification of human SH3 domain construct ITSN1-1/5-IS2/3DepositorInsertITSN1-1/5-IS2/3 (ITSN1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
HLH Bmal Pas 1-465-VenC I317D
Plasmid#47367PurposeBmal fragment cloned with Venus tag used for mutationDepositorInsertBMAL (Bmal1 Mouse)
UseTagsVenus aa 156-239ExpressionMammalianMutationaa 1-465, I317DPromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_LASP1-1/1-SV2/2
Plasmid#91392PurposeProtein expression and purification of human SH3 domain construct LASP1-1/1-SV2/2DepositorInsertLASP1-1/1-SV2/2 (LASP1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only