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Plasmid#17845DepositorInsertMirk (DYRK1B Human)
UseTags6xHis and Anti-XPressExpressionMammalianMutationY271/273FPromoterAvailable sinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSiMPlh_N + pSiMPlh_C
Plasmid#134316PurposeMaintenance of 2 plasmids with a single antibiotic in E. coliDepositorInserthygR_gp41-1 N-intein + hygR_gp41-1 C-intein
UseTagsExpressionBacterialMutationP254Q in hygR_gp41-1 N-inteinPromoterAvailable sinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
NSK024 SFFV-mCherry-SG(p53R248Q.s2)SG-EGFP (FLP-IN)
Plasmid#191169PurposeExpression of a sensor for p53 mutant R248Q in mammalian cells; mCherry marker, EGFP outputDepositorInsertmCherry:p53(R248Q).s2:EGFP
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSP2283
Plasmid#70702PurposeBacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1DepositorInsertsmammalian codon-optimized Staphylococcus aureus Cas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialMutationPromoterT7Available sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAID1.2-EF1a-NmScarlet-mAID
Plasmid#140617PurposeExpression of OsTIR1 and mScarlet-mAID protein in mammalian cells under the control of EF1a promoterDepositorInsertOsTIR1
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
p426 25Q GAL
Plasmid#1187DepositorInserthtt 25Q (HTT Human)
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPR-V2_mMSH2
Plasmid#186156PurposesgRNA targeting murine Msh2 geneDepositorInsertMsh2_gRNA (Msh2 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_Tiam1-pMag(3x)-iRFP
Plasmid#67305PurposeBlue light dependent photoswitching systemDepositorInsertTiam1-pMag(3x)-iRFP (Tiam1 Synthetic, Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJZC518
Plasmid#62277PurposeYeast dCas9 expression plasmidDepositorInsertdCas9
UseTagsExpressionYeastMutationHas mutations in the RuvC1 and HNH domains to ren…PromoterpTdh3Available sinceMarch 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDM1082
Plasmid#108982PurposeKnockout cassette G418DepositorInsertKnockout cassette G418
UseCre/Lox; Dictyostelium discoideum knockout vectorTagsExpressionMutationPromotercoaAAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
p426 72Q GPD
Plasmid#1183DepositorInserthtt 72Q (HTT Human)
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 RUVBL1
Plasmid#67091PurposeStandard for cell-free expression benchmarking.DepositorInsertRUVBL1 (RUVBL1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPI452
Plasmid#113203PurposeInducible expression vector (dox-on) N-terminal mScarlet taggingDepositorTypeEmpty backboneUseDictyostelium discoideum inducible expression vec…TagsmScarletExpressionMutationPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-dpcoCas9-EV2.1
Plasmid#158411PurposeCRISPR-dpcoCas9 fused with EDLL activator for transcriptional activationDepositorInsertdpcoCas9-EDLL-T2A-MS2-VPR(VP64-p65-Rta)-NOS-ter
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE Myc-epitope donor ORF0
Plasmid#200374PurposeTagging endogenously expressed proteins with Myc epitope, C-terminalDepositorInsertMyc epitope
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE Myc-epitope donor ORF1
Plasmid#200375PurposeTagging endogenously expressed proteins with Myc epitope, C-terminalDepositorInsertMyc epitope
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJEC581
Plasmid#174369PurposeRFP reporter used to evaluate CRISPRa in bacteria, contains gRNA target sites every 10 bp upstream of the promoter.DepositorInsertRFP with PAM rich sequence upstream promoter
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23117Available sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pER-eZinCh-2
Plasmid#70009Purposeexpresses ER-eZinCh-2 Zn2+ FRET sensor in the ER of mammalian cellsDepositorInsertER-eZinCh-2
UseTagsKDEL sequence and PPI signalExpressionMammalianMutationPromoterCMVAvailable sinceNov. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBW103ParsR-Amp30C
Plasmid#78638PurposepBW100ParsR carrying 30hrpR-30hrpS-t-hrpL-30gfp-tDepositorInsert30hrpR-30hrpS-t-hrpL-30gfp-t
UseSynthetic BiologyTagsExpressionMutationPromotersee insertAvailable sinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
p33Fn
Plasmid#158155PurposeExpression of crRNA array and FnCas12a using one Pol II promoter. For plant genome editing.DepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationRice codon optimizedPromoterAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only