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Plasmid#133273PurposeMammalian expression vector. It will generate the mouse TREK-1 channel full lenght K271Q mutant fused to a N-terminal HA tagDepositorInsertPotassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)
UseTagsHAExpressionMammalian and YeastMutationK271QPromoterAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
p28BIOHTEV-LIC: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2
Plasmid#167848PurposeBacterial expression of the SARS-CoV-2 proteins nsp10 and nsp16 from a bicistronic mRNADepositorInsertnsp10, nsp16
UseTags6xHis and AviTagExpressionBacterialMutationPromoterT7Available sinceApril 22, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX Nsp2-SH2
Plasmid#46460DepositorInsertNsp2 (BCAR3 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 144-259PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GRB2-1/2
Plasmid#91480PurposeProtein expression and purification of human SH3 domain construct GRB2-1/2DepositorInsertGRB2-1/2 (GRB2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GRB2-2/2
Plasmid#91410PurposeProtein expression and purification of human SH3 domain construct GRB2-2/2DepositorInsertGRB2-2/2 (GRB2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GRAP2-2/2
Plasmid#91368PurposeProtein expression and purification of human SH3 domain construct GRAP2-2/2DepositorInsertGRAP2-2/2 (GRAP2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
sgGFP_3
Plasmid#78165PurposesgGFPDepositorInsertGFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 p37 SHPmut
Plasmid#113503PurposeBacterial expression of GST-tagged p37 that is p97 binding deficient due to mutations in the SHP boxDepositorInsertp37 (Ubxn2b Mouse)
UseTagsGST-PreScission protease siteExpressionBacterialMutationF215A/E218A/QKL220-222AAAPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_EPS8L2-1/1
Plasmid#91526PurposeProtein expression and purification of human SH3 domain construct EPS8L2-1/1DepositorInsertEPS8L2-1/1 (EPS8L2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
KAT6B-Tol2
Plasmid#113384Purposeevaluate FOXA1 DIV motif enhancer activity near 50 kb of KAT6B geneDepositorInsertKAT6B-enhancer (KAT6B Human)
UseTol2 reporterTagsExpressionMutationPromoterAvailable sinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pfast bac cry1myc
Plasmid#51892PurposeBackbone plasmid is pfastBacHTa from invitrogenDepositorInsertcry1 (Cry1 Mouse)
UseTags3x Flag and mycExpressionMutationPromoterpolyhedrin promoterAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTKan-p35S::Csy4-pNOS::cogRFP (C134, JBEI-15901)
Plasmid#110146PurposeTransformation and expression of Csy4 and cogDsRed proteins in plantsDepositorInsertCsy4 (ATCS Mustard Weed)
UseTagscogRFPExpressionBacterial and PlantMutationPromoterAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX chimerin2-SH2
Plasmid#46416DepositorInsertchimerin2 (CHN2 Human)
UseTagsAviTag, GST, and PreScissionExpressionBacterialMutationcontains amino acids 49-155PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Mist-SH2 RK
Plasmid#46455DepositorInsertMist RK (Clnk Mouse)
UseTagsAviTag, GST, and PreScissionExpressionBacterialMutationcontains amino acids 300-435PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEBTetBl-CLIP-Cep70
Plasmid#136870PurposeMammalian expression of the centrosomal protein Cep70 N-terminally fused to CLIP-tagDepositorInsertCLIP-Cep70 (CEP70 Human, Synthetic)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ASAP1-1/1
Plasmid#91501PurposeProtein expression and purification of human SH3 domain construct ASAP1-1/1DepositorInsertASAP1-1/1 (ASAP1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEBTetDBl-CLIP-Cep70
Plasmid#136871PurposeMammalian expression of the centrosomal protein Cep70 N-terminally fused to CLIP-tagDepositorInsertCLIP-Cep70 (CEP70 Human, Synthetic)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTetBl-CLIP-Cep152
Plasmid#136874PurposeMammalian expression of the centrosomal protein Cep152 N-terminally fused to CLIP-tagDepositorInsertCLIP-Cep152 (CEP152 Human, Synthetic)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX Grb2-SH2 RK
Plasmid#46441DepositorInsertGrb2 RK (GRB2 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 58-159PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAPM-D4 miR30-AGO1 ts1
Plasmid#115850PurposeAGO1 knockdownDepositorInsertAGO1 shRNA (AGO1 Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only