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Plasmid#147890PurposeMammalian Expression of HsNot1iso1_1-1840-IKGRQtoDSYAA-shRNAresDepositorInsertHsNot1iso1_1-1840-IKGRQtoDSYAA-shRNAres (CNOT1 Human)
UseTagsExpressionMammalianMutation1 silent mutation compared to the sequence of iso…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSbi-pur-EGFP-KSR1-CA3 (N-KSR)
Plasmid#217769PurposeFluorescent reporter for glucosyl-ceramide (putative, mammalian expression)DepositorInsertKinase suppressor of ras 1 CA3 domain (KSR1 Human)
UseSleeping beauty transposase-compatible genome ins…TagsEGFPExpressionMammalianMutationCA3 domain aa 317-400PromoterEF-1alpha promoterAvailable sinceJune 10, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGL3-Basic-ACSL3-sfGFP(C) HDR template
Plasmid#129413PurposeHDR tempalte for tagging of endogenous human ACSL3 C-terminus with sfGFPDepositorInsertACSL3 HDR template (ACSL3 Human)
UseCRISPR and TALEN; Endogenous tagging hdr templateTagssfGFPExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSbi-pur-KSR1-CA3-EGFP (C-KSR)
Plasmid#217768PurposeFluorescent reporter for ceramide (for stable mammalian expression)DepositorInsertKinase suppressor of ras 1 CA3 domain (KSR1 Human)
UseSleeping beauty transposase-compatible genome ins…TagsEGFPExpressionMammalianMutationCA3 domain aa 317-400 plus vector-based linker LE…PromoterEF-1alpha promoterAvailable sinceJune 10, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-EF1α-FRT-FLEX-GtACR2-KA2N-EGFP
Plasmid#114378Purposeexpresses Flpo recombinase-dependent GtACR2-KA2N, which targets GtACR2 to the somatodendritic compartmentDepositorInsertGtACR2-KA2N-EGFP (NEWENTRY )
UseAAVTagsEGFP and KA2NExpressionMammalianMutationPromoterEf1aAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pME nls EGFP WPRE bGH pA (JDW 488)
Plasmid#224540PurposeA Gateway compatible middle entry clone containing a 3x-NLS-EGFP for labeling the nucleusDepositorInsertnls-EGFP-WPRE-bGH-pA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot3-del698-725-F764E-dsRNAres_U
Plasmid#147704PurposeInsect Expression of DmNot3-del698-725-F764E-dsRNAresDepositorInsertDmNot3-del698-725-F764E-dsRNAres (Not3 Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1mut_R
Plasmid#147395PurposeInsect Expression of CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1mutDepositorInsertCeAIN2_254-400-HsTNRC6C_1207-1690-CIM1mut (TNRC6C Nematode, Human)
UseTagsExpressionInsectMutationDeletion of 2 AA (SD) at position 378-379 compare…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CeAIN2_254-400-HsTNRC6C_1207-1690-F1389A_R
Plasmid#147398PurposeInsect Expression of CeAIN2_254-400-HsTNRC6C_1207-1690-F1389ADepositorInsertCeAIN2_254-400-HsTNRC6C_1207-1690-F1389A (TNRC6C Nematode, Human)
UseTagsExpressionInsectMutationDeletion of 2 AA (SD) at position 378-379 compare…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CeAIN2_254-400-HsTNRC6C_1207-1690-P-GL-mut_R
Plasmid#147399PurposeInsect Expression of CeAIN2_254-400-HsTNRC6C_1207-1690-P-GL-mutDepositorInsertCeAIN2_254-400-HsTNRC6C_1207-1690-P-GL-mut (TNRC6C Nematode, Human)
UseTagsExpressionInsectMutationDeletion of 2 AA (SD) at position 378-379 compare…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGW182_1-1199-del862-1115-dsRNAres_L
Plasmid#146820PurposeInsect Expression of DmGW182_1-1199-del862-1115-dsRNAresDepositorInsertDmGW182_1-1199-del862-1115-dsRNAres (gw Fly)
UseTagsExpressionInsectMutationTwo non silent mutations V704A and N799S and one …PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_205-830-dsRNAres_S
Plasmid#147513PurposeInsect Expression of CeAIN2_254-400-DmGW182_205-830-dsRNAresDepositorInsertCeAIN2_254-400-DmGW182_205-830-dsRNAres (gw Fly, Nematode)
UseTagsExpressionInsectMutationDeletion of 2 AA (SD) at position 378-379 compare…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-M2WA_R
Plasmid#147387PurposeInsect Expression of CeAIN2_254-400-DmGW182_635-1384-M2WADepositorInsertCeAIN2_254-400-DmGW182_635-1384-M2WA (gw Fly, Nematode)
UseTagsExpressionInsectMutationDeletion of 2 AA (SD) at position 378-379 compare…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-P-GLmut_R
Plasmid#147390PurposeInsect Expression of CeAIN2_254-400-DmGW182_635-1384-P-GLmutDepositorInsertCeAIN2_254-400-DmGW182_635-1384-P-GLmut (gw Nematode, Human)
UseTagsExpressionInsectMutationDeletion of 2 AA (SD) at position 378-379 compare…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_1-634-dsRNAres_R
Plasmid#147382PurposeInsect Expression of CeAIN2_254-400-DmGW182_1-634-dsRNAresDepositorInsertCeAIN2_254-400-DmGW182_1-634-dsRNAres (gw Fly, Nematode)
UseTagsExpressionInsectMutationDeletion of 2 AA (SD) at position 378-379 compare…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_205-490-dsRNAres_R
Plasmid#147383PurposeInsect Expression of CeAIN2_254-400-DmGW182_205-490-dsRNAresDepositorInsertCeAIN2_254-400-DmGW182_205-490-dsRNAres (gw Fly, Nematode)
UseTagsExpressionInsectMutationDeletion of 2 AA (SD) at position 378-379 compare…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1_1-1717-del1156-1169-R1197AN1203AF1204A_X
Plasmid#147984PurposeInsect Expression of DmNot1_1-1717-del1156-1169-R1197AN1203AF1204ADepositorInsertDmNot1_1-1717-del1156-1169-R1197AN1203AF1204A (Not1 Fly)
UseTagsExpressionInsectMutation6 silent mutations and two non silent N605S, K759…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1_1-1717-del1156-1169-RNFKGQtoAAASEA_X
Plasmid#147985PurposeInsect Expression of DmNot1_1-1717-del1156-1169-RNFKGQtoAAASEADepositorInsertDmNot1_1-1717-del1156-1169-RNFKGQtoAAASEA (Not1 Fly)
UseTagsExpressionInsectMutation6 silent mutations and two non silent N605S, K759…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1-del1156-1169-RNFtoAAA-dsRNAres_W
Plasmid#147859PurposeInsect Expression of DmNot1-del1156-1169-RNFtoAAA-dsRNAresDepositorInsertDmNot1-del1156-1169-RNFtoAAA-dsRNAres (Not1 Fly)
UseTagsExpressionInsectMutation10 silent mutations and two non silent N605S, K75…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCBh_NLS_dCas13X_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA
Plasmid#191796Purposevector for encoding a human codon-optimized dead Cas13X (dCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoterDepositorInserthumanized dCas13X
UseCRISPRTagsExpressionMammalianMutationR84A, H89A, R739A, R740A, H745A, H746A, H747APromoterCBh, SV40, hU6Available sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only