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pT7-EGFP-C1-HsNot1iso1-del1097-1110_W

Plasmid

#147836

Purpose

Mammalian Expression of HsNot1iso1-del1097-1110

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1-del1097-1110 (CNOT1 Human)

Use

Tags

Expression

Mammalian

Mutation

1 silent mutation compared to the sequence of iso…

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1-IKGIRQtoDSYDAA-shRNAres_W

Plasmid

#147837

Purpose

Mammalian Expression of HsNot1iso1-IKGIRQtoDSYDAA-shRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1-IKGIRQtoDSYDAA-shRNAres (CNOT1 Human)

Use

Tags

Expression

Mammalian

Mutation

1 silent mutation compared to the sequence of iso…

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1_1318-1605-IKGIRQtoDSYDAA_W

Plasmid

#147894

Purpose

Mammalian Expression of HsNot1iso1_1318-1605-IKGIRQtoDSYDAA

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1_1318-1605-IKGIRQtoDSYDAA (CNOT1 Human)

Use

Tags

Expression

Mammalian

Mutation

1 silent mutation compared to the sequence of iso…

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1-K1537SG1562EQ1658A-dsRNAres_W

Plasmid

#147860

Purpose

Insect Expression of DmNot1-K1537SG1562EQ1658A-dsRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1-K1537SG1562EQ1658A-dsRNAres (Not1 Fly)

Use

Tags

Expression

Insect

Mutation

10 silent mutations and two non silent N605S, K75…

Promoter

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1_812-1945-dsRNAres_W

Plasmid

#147845

Purpose

Insect Expression of DmNot1_812-1945-dsRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1_812-1945-dsRNAres (Not1 Fly)

Use

Tags

Expression

Insect

Mutation

3 silent mutations compared to the sequence given…

Promoter

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmTral_394-543-V5His6_D

Plasmid

#146134

Purpose

Insect Expression of DmTral_394-543

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral_394-543 (tral Fly)

Use

Tags

Expression

Insect

Mutation

two silent mutations (nucleotides A198C, A387G) a…

Promoter

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1_1147-1717-3xL-Amut_P

Plasmid

#147161

Purpose

Insect Expression of DmNot1_1147-1717-3xL-Amut

Depositor

Elisa Izaurralde

Insert

DmNot1_1147-1717-3xL-Amut (Not1 Fly)

Use

Tags

Expression

Insect

Mutation

2 silent mutations compared to the sequence given…

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmSmaug-del584-762-V5His6_C

Plasmid

#146006

Purpose

Insect Expression of DmSmaug-del584-762

Depositor

Elisa Izaurralde

Insert

DmSmaug-del584-762 (smg Fly)

Use

Tags

Expression

Insect

Mutation

7 silent mutations and a deletion of the AA 961-9…

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1_1318-1605-KGRQtoSYAA_W

Plasmid

#147896

Purpose

Mammalian Expression of HsNot1iso1_1318-1605-KGRQ-SYAA

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1_1318-1605-KGRQ-SYAA (CNOT1 Human)

Use

Tags

Expression

Mammalian

Mutation

1 silent mutation compared to the sequence of iso…

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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BE3(E63Q)-P2A-EGFP (pRZ189)

Plasmid

#123613

Purpose

CAG promoter expression plasmid for rAPOBEC1(E63Q)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (catalytically impaired BE3 mutant).

Depositor

Keith Joung

Article

Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

Insert

BE3(E63Q)-P2A-EGFP

Use

Tags

Expression

Mammalian

Mutation

E63Q in rAPOBEC1, D10A in SpCas9

Promoter

CAG

Available since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1-3xL-Amut-dsRNAres_P

Plasmid

#147160

Purpose

Insect Expression of DmNot1-3xL-Amut-dsRNAres

Depositor

Elisa Izaurralde

Insert

DmNot1-3xL-Amut-dsRNAres (Not1 Fly)

Use

Tags

Expression

Insect

Mutation

10 silent mutations and two non silent N605S, K75…

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pFS_0271_pET30_pCat-tetR-Term-ptetA-sfGFP-rrnBterm_Fusicatenibacter_saccharivorans_ARRAY2_FaqI

Plasmid

#116955

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac and sfGFP under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmHPat-del57-499-dsRNAres_J

Plasmid

#146691

Purpose

Insect Expression of DmHPat-del57-499-dsRNAres

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmHPat-del57-499-dsRNAres (Patr-1 Fly)

Use

Tags

Expression

Insect

Mutation

one silent mutation A1758G compared to the sequen…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pME-Sun1-2xsfGFP-6xMYC-pA (JDW 681)

Plasmid

#224489

Purpose

Gateway compatible middle entry clone containing Sun1 reporter fusion to 2 copies of sfGFP and 6 MYC tags (Nuclear envelope reporter and affinity tag)

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

Sun1-2xsfGFP-6xMYC-pA

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pME Actin-Vhh sfGFP (JDW 1215)

Plasmid

#224495

Purpose

Gateway compatible middle entry clone with Actin nanobody fused to sfGFP (GFP actin reporter)

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

Actin-Vhh-sfGFP

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pME-EGFP-P2A-iCre (JDW 921)

Plasmid

#224519

Purpose

A Gateway compatible middle entry clone containing EGFP P2A iCre (codon optimized cre)

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

EGFP-P2A-iCre

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pME-Lifeact-EGFP-3xHA (JDW 1322)

Plasmid

#224494

Purpose

Gateway compatible middle entry clone containing Lifeact-EGFP (EGFP F-actin reporter)

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

Liefact-EGFP-3xHA

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pME nls EGFP-Cre (JDW 1329)

Plasmid

#224515

Purpose

A Gateway compatible middle entry clone containing a NLS-EGFP-Cre fusion

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

nls-EGFP-Cre

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-Puro-hTBK1-E696K-EGFP

Plasmid

#221554

Purpose

Expresses GFP-tagged human TBK1 with E696K mutation in mammalian cells. Compatible with piggybac transposase for genome integration.

Depositor

Shawn Ferguson

Article

Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

Insert

TBK1 (TBK1 Human)

Use

Piggybac

Tags

GFP

Expression

Mammalian

Mutation

Changed glutamate 696 to lysine

Promoter

EF1a

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-LAMP-1-mGFP-IRES-Puromycin

Plasmid

#134868

Purpose

Expresses EGFP-tagged lysosomal marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Lysosomal-associated membrane protein 1 (LAMP1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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