Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Showing: 1101 - 1120 of 3953 results

  1. pSynSRE-Mut-T-Luc

    Plasmid
    #60490
    Purpose
    Contains a mutant version of the WT pSynSRE-T-Luc reporter plasmid. Four point mutations in the promoter SRE elements abolish SREBP-induced luciferase expression as described by Smith et al., 1988
    Depositor
    Timothy Osborne
    Article
    Smith et al J Biol Chem. 1988 Dec 5;263(34):18480-7.
    Insert
    HMG-CoA synthase promoter (-324/-225) fused to HMG-CoA synthase TATAA (-28/+39) transcription initiator sequence
    Use
    Luciferase
    Tags
    Luciferase reporter
    Expression
    Mammalian
    Mutation
    Four point mutations (G->C or A->C) in the …
    Promoter
    Partial HMG-CoA synthase promoter (-324/-225) fus…
    Availability
    Academic Institutions and Nonprofits only
    60490-plasmid-map-sequence-id-332535

    Enlarge

  2. pLV-EF1A-WWE-HA-nLuc-FLAG-T2A-WWE-myc-cLuc-FLAG-IRES-Puro

    Plasmid
    #187611
    Purpose
    Split luciferase construct with nLuc fused to C-terminus of WWE domain, linked by T2A to cLuc fused to C-terminus of WWE domain, linked by IRES to puromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cancers (Basel). 2022 Jul 28;14(15). pii
    Inserts
    WWE domain with C-terminal nLuc
    WWE domain with C-terminal cLuc
    Use
    Lentiviral
    Tags
    FLAG, HA, Split luciferase (cLuc), Split lucifera…
    Expression
    Mammalian
    Mutation
    Promoter
    EF1A
    Availability
    Academic Institutions and Nonprofits only
    187611-plasmid-map-sequence-id-372189

    Enlarge

  3. pLV-EF1A-WWE(R163A)-HA-nLuc-FLAG-T2A-WWE-myc-cLuc-FLAG-IRES-Puro

    Plasmid
    #187612
    Purpose
    Split luciferase construct with nLuc fused to C-terminus of WWE domain with Arg163Ala mutation, linked by T2A to cLuc fused to C-terminus of WWE domain, linked by IRES to puromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cancers (Basel). 2022 Jul 28;14(15). pii
    Inserts
    WWE domain (R163A) with C-terminal nLuc
    WWE domain with C-terminal cLuc
    Use
    Lentiviral
    Tags
    FLAG, HA, Split luciferase (cLuc), Split lucifera…
    Expression
    Mammalian
    Mutation
    Arg163Ala
    Promoter
    EF1A
    Availability
    Academic Institutions and Nonprofits only
    187612-plasmid-map-sequence-id-372035

    Enlarge

  11. psicheck_300_400_CA_repeat

    Plasmid
    #65879
    Purpose
    Luciferase assay vector, assessing regulation of 300-400 nucleotide CA repeats
    Depositor
    Thomas Tuschl
    Insert
    CA repeats 300-400 nucleotides in length
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  12. psicheck_200_300_CA_repeat

    Plasmid
    #65880
    Purpose
    Luciferase assay vector, assessing regulation of 200-300 nucleotide CA repeats
    Depositor
    Thomas Tuschl
    Insert
    CA repeats 200-300 nucleotides in length
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  13. psicheck_50_100_CA_repeat

    Plasmid
    #65882
    Purpose
    Luciferase assay vector, assessing regulation of 50-100 nucleotide CA repeats
    Depositor
    Thomas Tuschl
    Insert
    CA repeats 50-100 nucleotides in length
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  14. pGL3-Basic-OPN-600

    Plasmid
    #11997
    Depositor
    Steven Johnson
    Insert
    Osteopontin (SPP1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    The following construct contains the OPN promoter…
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  15. p_L_3c_SG_RLuc

    Plasmid
    #208729
    Purpose
    For assessing leaky scanning rates in mammalian cells
    Depositor
    Andrew Firth
    Insert
    ORF3c (ORF3a )
    Use
    Tags
    Renilla luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    208729-plasmid-map-sequence-id-420912

    Enlarge

  16. pAAV-hSyn-LMO11 (GeNL_SS-ChRmine)

    Plasmid
    #213034
    Purpose
    fusion protein of mNeonGreen, SSLuc, and ChRmine for bioluminescent optogenetics
    Depositor
    Ute Hochgeschwender
    Inserts
    mNeonGreen
    ISN-IE
    ChRmine
    Use
    AAV
    Tags
    Gaussia luciferase signal sequence
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    213034-plasmid-map-sequence-id-435311

    Enlarge

Showing: 1101 - 1120 of 3953 results