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We narrowed to 5,390 results for: golden gate

Showing: 1101 - 1120 of 5390 results
  1. C114m

    Plasmid
    #121013
    Purpose
    MoClo golden gate assembly CD part for Cas9 (S. pyogenes gRNA-targeted endonuclease Cas9, with bbsI cut sites removed synonymously). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Insert
    dCas9 with no bbsI or BsaI sites
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
  2. PB_gRNA_S.pyogenes_scaffold_BB

    Plasmid
    #226429
    Purpose
    PiggyBac transposon vector backbone for cloning of sgRNA compatible with S.pyogenes dCas9 enzyme. BbsI Golden Gate site upstream of the scaffold for protospacer insertion. Puromycin selection marker.
    Depositor
    Type
    Empty backbone
    Use
    CRISPR; Piggybac transposon
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Sept. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  3. pUAP1

    Plasmid
    #63674
    Purpose
    Accepts DNA sequences via BpiI cloning sites, resulting in Level 0 standard parts for Golden Gate cloning. Parts can be released with a user-defined 4-base-pair, 5 prime overhangs using BsaI.
    Depositor
    Insert
    RFP cloning selection cassette
    Use
    Synthetic Biology
    Available since
    April 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  4. pYL001

    Plasmid
    #249611
    Purpose
    Cloning vector for PaqCI Golden Gate assembly of ISYmu1 gRNAs into TRV2 with mutation SlFT element
    Depositor
    Type
    Empty backbone
    Expression
    Plant
    Available since
    Jan. 12, 2026
    Availability
    Academic Institutions and Nonprofits only
Showing: 1101 - 1120 of 5390 results