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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3,824 results for: golden gate

Showing: 1101 - 1120 of 3824 results

  1. pVL1172

    Plasmid
    #239535
    Purpose
    Express CpPT1 membrane protein cargo with C-terminal mTurqoise2 marker but without any targeting sequence and Venus ER marker
    Depositor
    John Dueber
    Insert
    CpPT1(52-408)-mTurquoise2
    Expression
    Yeast
    Available Since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    239535-plasmid-map-sequence-id-476352

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  2. pKS1619

    Plasmid
    #239550
    Purpose
    Express GAL-inducible cytochrome P450 reductase tagged with mTagBFP2 and Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    CrCPR-mTagBFP2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239550-plasmid-map-sequence-id-475997

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  3. pKS1601

    Plasmid
    #239526
    Purpose
    Express CpPT1(52-408) membrane enzyme cargo and Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    2xCrk_SH3-mTurquoise2-CpPT1(52-408)
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239526-plasmid-map-sequence-id-475905

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  4. pKS1531

    Plasmid
    #239521
    Purpose
    Express truncated VoGES and VvGT14 in the peroxisome for synthesis of geranyl _-glucoside; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    tVoGES-ePTS1
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239521-plasmid-map-sequence-id-475896

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  5. pKS1575

    Plasmid
    #239523
    Purpose
    Express Vba2p membrane protein cargo fused directly to Pex22 TMD, peroxisomal Ynd1p truncation, and peroxisomal mRuby2
    Depositor
    John Dueber
    Insert
    ScPex22(1-36)-ScVba2p-mTurquoise2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239523-plasmid-map-sequence-id-475887

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  6. pVL1337

    Plasmid
    #239541
    Purpose
    Express Yea4p membrane protein cargo fused directly to Pex15 TMD, peroxisomal Ynd1p truncation, and peroxisomal mRuby2
    Depositor
    John Dueber
    Insert
    ScYea4p-mTurquoise2-ScPex15(315-383)
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239541-plasmid-map-sequence-id-475879

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  7. pKS1574

    Plasmid
    #239522
    Purpose
    Express Yea4p membrane protein cargo fused directly to Pex22 TMD, peroxisomal Ynd1p truncation, and peroxisomal mRuby2
    Depositor
    John Dueber
    Insert
    ScPex22(1-36)-ScYea4p-mTurquoise2
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239522-plasmid-map-sequence-id-475877

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  8. pAIP4_3W4Q

    Plasmid
    #234236
    Purpose
    Heterologous protein expression of beta-lactamase in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3W4Q
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234236-plasmid-map-sequence-id-464200

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  9. pAIP4_3ILS

    Plasmid
    #234215
    Purpose
    Heterologous protein expression of norsolorinic acid synthase (NSAS) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3ILS
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234215-plasmid-map-sequence-id-464180

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  10. pAIP4_5NJO

    Plasmid
    #234202
    Purpose
    Heterologous protein expression of putative cupin_2 domain-containing isomerase in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5NJO
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234202-plasmid-map-sequence-id-464167

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  11. pAIP4_1ZHV

    Plasmid
    #234197
    Purpose
    Heterologous protein expression of aspartate kinase in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1ZHV
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234197-plasmid-map-sequence-id-464162

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  12. pAIP4_1NG6

    Plasmid
    #234191
    Purpose
    Heterologous protein expression of YqeY in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1NG6 (yqeY Bacillus subtilis)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234191-plasmid-map-sequence-id-464156

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  13. pAIP4_4QB4

    Plasmid
    #234187
    Purpose
    Heterologous protein expression of thermonuclease (TNase) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4QB4
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234187-plasmid-map-sequence-id-464152

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  14. pAIP4_2OHW

    Plasmid
    #234178
    Purpose
    Heterologous protein expression of YueI in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2OHW (yueI Bacillus subtilis)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234178-plasmid-map-sequence-id-464143

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  15. pAIP4_4CFI

    Plasmid
    #234163
    Purpose
    Heterologous protein expression of flagellin in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4CFI (QUF21_RS17235 Burkholderia pseudomallei K96243)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234163-plasmid-map-sequence-id-464129

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  16. L1P139 - pSPCH-PhiC31-tUBQ1

    Plasmid
    #219703
    Purpose
    Level 1 pSPCH-PhiC31-tUBQ1, for early stage of stomata development expression of PhiC31 integrase
    Depositor
    Jennifer Nemhauser
    Insert
    pSPCH:PhiC31:tUBQ1
    Expression
    Plant
    Available Since
    June 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    219703-plasmid-map-sequence-id-435733

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  17. L0-T15 BpiI-p35s-BpiI

    Plasmid
    #219698
    Purpose
    Level 0 to insert p35S promoter to history-dependent target
    Depositor
    Jennifer Nemhauser
    Insert
    BpiI-p35s-BpiI
    Use
    Synthetic Biology
    Available Since
    June 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    219698-plasmid-map-sequence-id-435729

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  18. pBD026

    Plasmid
    #201309
    Purpose
    AvrSr27-2 as Golden Gate compatible MoClo CDS part
    Depositor
    Benjamin Schwessinger
    Insert
    AvrSr27-2
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Oct. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    201309-plasmid-map-sequence-id-410982

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  19. pBD025

    Plasmid
    #201308
    Purpose
    AvrSr27-1 as Golden Gate compatible MoClo CDS part
    Depositor
    Benjamin Schwessinger
    Insert
    AvrSr27-1
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    201308-plasmid-map-sequence-id-409510

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  20. T18m

    Plasmid
    #121037
    Purpose
    MoClo golden gate assembly DE part for L3S2P21 (382x-strength synthetic terminator with BsaI cut site removed; see DOI: 10.1038/nmeth.2515).
    Depositor
    Richard Murray
    Insert
    modified L3S2P21 (synthetic Voigt terminator)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121037-plasmid-map-sequence-id-236351

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Showing: 1101 - 1120 of 3824 results