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pFRT_TO_eGFP_ELAVL1

Plasmid

#106105

Purpose

Inducible gene expression of EGFP ELAVL1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

NP_001410.2 (ELAVL1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCSegfpcherry

Plasmid

#22465

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

EGFP-mCherry

Use

Injection construct

Tags

Expression

Mutation

Promoter

Available since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pOmnibac_ZZ_linker_K560_GFP_SNAP

Plasmid

#159720

Purpose

Expresses kinesin-1 (amino acids 1-560) in Sf9 cells with a C-terminal GFP and SNAP tag

Depositor

Ahmet Yildiz

Article

Ferro et al Science. 2022 Jan 21;375(6578):326-331.

Insert

KIF5B (only amino acids 1-560), K560 (KIF5B Human)

Use

Tags

GFP and SNAP tags and ZZ affinity tag

Expression

Insect

Mutation

Only amino acids 1-560

Promoter

Available since

Jan. 21, 2022

Availability

Academic Institutions and Nonprofits only

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PA-sfGFP-N1

Plasmid

#54736

Purpose

Localization: N1 Cloning Vector, Excitation: 400 / 485, Emission: 468 / 511

Depositor

Michael Davidson

Type

Empty backbone

Use

Tags

PA-sfGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pAKgfp2

Plasmid

#14077

Depositor

Attila Karsi

Article

Karsi et al Plasmid. 2007 May;57(3):286-95. doi: 10.

Insert

lacIq repressor gene

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 12, 2007

Availability

Academic Institutions and Nonprofits only
pSLQ5079_pHR_PGK_sfGFP_CoV-F1

Plasmid

#155303

Purpose

SARS-CoV-2 fluorescent reporter 1

Depositor

Stanley Qi

Article

Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

Insert

Superfolder GFP fused with SARS-CoV-F1 (ORF1ab Synthetic)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiGuide_GFP_NT135

Plasmid

#183118

Purpose

pLentiGuide modified to express GFP and a non-targeting sgRNA

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

NT135

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pOxyR_gfp

Plasmid

#194154

Purpose

Green Fluorescent Reporter of intracellular hydrogen peroxide

Depositor

Sven Panke

Article

Kardashliev et al Chem Sci. 2021 Oct 29;12(44):14766-14772

Insert

transcriptional regulator, OxyR, cognate promoter PoxyS, fluorescent protein sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

PoxyR, PoxyS

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_NSD1_ZNF346

Plasmid

#205870

Purpose

Express mEGFP-tagged fusion protein, NSD1_ZNF346 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

NSD1_ZNF346 (NSD1, ZNF346 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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bfloGFPa1

Plasmid

#60749

Purpose

Bacterial expression of Amphioxus GFPa1

Depositor

Dimitri Deheyn

Article

Bomati et al Sci Rep. 2014 Jun 27;4:5469. doi: 10.103

Insert

GFPa1

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only
acta2_GFP_pA_pDestTolpA2

Plasmid

#78684

Purpose

acta2:GFP transgenesis construct

Depositor

Sarah Childs

Article

Whitesell et al PLoS One. 2014 Mar 3;9(3):e90590. doi: 1

Insert

acta2 promoter (acta2 Zebrafish)

Use

Unspecified

Tags

Expression

Mutation

Promoter

acta2

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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RFN_EGFP_81

Plasmid

#60073

Purpose

Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

Depositor

Keith Joung

Article

Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

Insert

pair of multiplex gRNAs targeting EGFP

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 16, 2014

Availability

Academic Institutions and Nonprofits only
pCRISPR_eGFP_191

Plasmid

#111821

Purpose

Knockout eGFP

Depositor

Steve Shih

Article

Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

Insert

gRNA targeting eGFP 171-191

Use

CRISPR

Tags

mCherry

Expression

Bacterial and Mammalian

Mutation

Promoter

U6

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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SIN40C.TRE.mArid3a3xFLAG.IRES.dTomato.PGK.sfGFP.P2A.Tet3G

Plasmid

#174666

Purpose

Doxycycline-inducible expression of murine Arid3a-3xFLAG cDNA with dTomato as reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

Arid3a-3xFLAG (Arid3a Mouse)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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iGFP

Plasmid

#66589

Purpose

inverted GFP reporter

Depositor

Wen Xue

Article

Li et al Genome Biol. 2015 May 28;16(1):111.

Insert

GFP

Use

Tags

Expression

Mutation

Promoter

CMV

Available since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pTD103luxI_sfGFP -R

Plasmid

#48887

Purpose

Positive feedback plasmid from oscillator without luxR

Depositor

Jeff Hasty

Article

Prindle et al Nature. 2011 Dec 18;481(7379):39-44. doi

Insert

sfGFP and luxI

Use

Synthetic Biology

Tags

ssrA tag (AANDENYALAA)

Expression

Bacterial

Mutation

Promoter

pLux

Available since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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2xmEGFP

Plasmid

#127799

Purpose

Homo-dimer mEGFP-mEGFP expression vector

Depositor

Salvatore Chiantia

Article

Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

Insert

mEGFP-mEGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_TMPRSS2_ETV4

Plasmid

#205945

Purpose

Express mEGFP-tagged fusion protein, TMPRSS2_ETV4 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

TMPRSS2_ETV4 (TMPRSS2, ETV4 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-8x

Plasmid

#172284

Purpose

Expressing EGFP mRNA fused with 8 tandem repeats of a 50-base sequence

Depositor

Antony K Chen

Article

Chen et al Sci Rep. 2017 May 8;7(1):1550. doi: 10.1

Insert

EGFP-N1-8x

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pJK_proB_eGFP

Plasmid

#59845

Purpose

Constitutively expresses eGFP with proB promoter

Depositor

Timothy Whitehead

Article

Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

Insert

Enhanced Green Fluorescent Protein

Use

Tags

6x-His

Expression

Bacterial

Mutation

S2R,S65G,S72A

Promoter

proB

Available since

Oct. 20, 2014

Availability

Academic Institutions and Nonprofits only

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