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pCEP4-HA-Flag-natT1R2 ECD

Plasmid

#113954

Purpose

mammalian expression plasmid for FLAG-tagged human T1R2 extracellular domain with signal peptide of influenza hemagglutinin

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R2 (TAS1R2 Human)

Use

Tags

FLAG and Signal/leader sequence from influenza he…

Expression

Mammalian

Mutation

residues 22-568

Promoter

cmv

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pX330.Pten.a

Plasmid

#66587

Purpose

sgRNA for Pten deletion

Depositor

Wen Xue

Article

Li et al Genome Biol. 2015 May 28;16(1):111.

Insert

Pten deletion (Pten Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
PVT1-Tol2

Plasmid

#113381

Purpose

evaluate FOXA1 DIV motif enhancer activity near 50 kb of PVT1 gene

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

PVT1-enhancer (PVT1 Human)

Use

Tol2 reporter

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only
pLG1-puro-sgATL3-5

Plasmid

#109011

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL3-sgRNA #5 (ATL3 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6 promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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mCit-Snap25b D179K A199R

Plasmid

#53259

Purpose

SNAP25B double mutant insensitive to both Botulinum toxin C1 & E; N-terminal mCitrine tag connected by 4 amino acid linker. For mammalian cells, used with mCer-4aa-VAMP2 to measure FRET & dispersion.

Depositor

Robert Zucker

Article

Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

Insert

SNAP-25B (Snap25 Rat)

Use

Tags

4aa-linker and mCitrine

Expression

Mammalian

Mutation

D179K and A199R

Promoter

CMV

Available since

April 21, 2015

Availability

Academic Institutions and Nonprofits only
pTet02

Plasmid

#78284

Purpose

mRFP1 expressed behind an ATc-inducible construct

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

Tet inducible mRFP1

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pJRTCLCrVA009

Plasmid

#22842

Depositor

Niki Robertson

Article

Tuttle et al Plant Physiol. 2008 Sep . 148(1):41-50.

Insert

A DNA containing CHLI fragment

Use

Cotton vigs vector

Tags

Expression

Bacterial

Mutation

Cotton Leaf Crumple Virus Coat Protein (AR1 gene)…

Promoter

Available since

June 14, 2010

Availability

Academic Institutions and Nonprofits only

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pFBD-Akt1(D323A/D325A)

Plasmid

#86592

Purpose

Expresses human full-length Akt1(D323A/D325A) mutant

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Use

Tags

10xHis

Expression

Insect

Mutation

D323A/D325A transforming mutations

Promoter

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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MIGR1_FLAG_TAL1-long_dt tomato

Plasmid

#210639

Purpose

Overexpression of TAL1-long

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1-long (TAL1 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pMMK ENTR 4 CMV eGFP

Plasmid

#206272

Purpose

ENTR Vector 4 for MultiSite Gateway assembly. Encodes eGFP under the control of a CMV promoter

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

eGFP

Use

Multimate/gateway entr 4

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pMMK ENTR 4 CMV mTagBFP

Plasmid

#206279

Purpose

ENTR Vector 4 for MultiSite Gateway assembly. Encodes mTagBFP under the control of a CMV promoter.

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

mTagBFP

Use

Multimate/gateway entr 4

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsSmg6-sh(t10)RNAres_S

Plasmid

#147460

Purpose

Mammalian Expression of HsSmg6-sh(10)RNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsSmg6-sh(10)RNAres (SMG6 Human)

Use

Tags

Expression

Mammalian

Mutation

two silent mutations T594C, A699G and two non sil…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsSmg7-siRNAres-shRNAres_S

Plasmid

#147463

Purpose

Mammalian Expression of HsSmg7-siRNAres-shRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsSmg7-siRNAres-shRNAres (SMG7 Human)

Use

Tags

Expression

Mammalian

Mutation

three silent mutation G216A, T1185C and A1521G an…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pN1-HsSmg7-G100E-siRNAres-V5-SBP-MBP_S

Plasmid

#147472

Purpose

Mammalian Expression of HsSmg7-G100E-siRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsSmg7-G100E-siRNAres (SMG7 Human)

Use

Tags

Expression

Mammalian

Mutation

three silent mutation G216A, T1185C, A1521G and o…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pN1-HsSmg7-K66ER163E-siRNAres-V5-SBP-MBP_S

Plasmid

#147473

Purpose

Mammalian Expression of HsSmg7-K66ER163E-siRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsSmg7-K66ER163E-siRNAres (SMG7 Human)

Use

Tags

Expression

Mammalian

Mutation

three silent mutation G216A, T1185C, A1521G and o…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pN1-HsSmg7-siRNAres-V5-SBP-MBP_S

Plasmid

#147474

Purpose

Mammalian Expression of HsSmg7-siRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsSmg7-siRNAres (SMG7 Human)

Use

Tags

Expression

Mammalian

Mutation

three silent mutation G216A, T1185C, A1521G and o…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HsUpf1_115-1129-S1107A-siRNAres_S

Plasmid

#147477

Purpose

Mammalian Expression of HsUpf1_115-1129-S1107A-siRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsUpf1_115-1129-S1107A-siRNAres (UPF1 Human)

Use

Tags

Expression

Mammalian

Mutation

4 non silent mutations E167G, K282R, A442G and T1…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HsUpf1-S1107A-siRNAres_S

Plasmid

#147478

Purpose

Mammalian Expression of HsUpf1-S1107A-siRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsUpf1-S1107A-siRNAres (UPF1 Human)

Use

Tags

Expression

Mammalian

Mutation

5 non silent mutations A69S, E167G, K282R, A442G…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-TBG-Cre-sgArid1a

Plasmid

#192165

Purpose

AAV-TBG-Cre-sgArid1a

Depositor

Sidi Chen

Article

Wang et al Cancer Discov. 2020 Dec;10(12):1912-1933

Type

Empty backbone

Use

AAV

Tags

Expression

Mutation

NA

Promoter

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-TBG-Cre-sgKmt2d

Plasmid

#192164

Purpose

AAV-TBG-Cre-sgKmt2d

Depositor

Sidi Chen

Article

Wang et al Cancer Discov. 2020 Dec;10(12):1912-1933

Type

Empty backbone

Use

AAV

Tags

Expression

Mutation

NA

Promoter

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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