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Showing: 1101 - 1120 of 20410 results

  1. pCgVchCAST-crtYf

    Plasmid
    #203816
    Purpose
    Expression of CRISPR-associated transposases, shuttle vector for Corynebacterium glutamicum / E. coli
    Depositor
    Sheng Yang
    Insert
    VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(crtYf)
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    203816-plasmid-map-sequence-id-403367

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  2. pCRISPathBrick

    Plasmid
    #65006
    Purpose
    E. coli vector for expression of S. pyogenes dCas9, tracrRNA, and nontargeting CRISPR array with BsaI site for inserting user-defined spacer-repeat bricks
    Depositor
    Mattheos Koffas
    Article
    Cress et al ACS Synth Biol. 2015 Mar 30.
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive native promoters
    Availability
    Academic Institutions and Nonprofits only
    65006-plasmid-map-sequence-id-109903

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  3. pSL0762 (pQCascade_crRNA-nt)

    Plasmid
    #130636
    Purpose
    Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array encodes a non-targeting guide RNA.
    Depositor
    Samuel H. Sternberg
    Article
    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(non-targeting)
    Use
    CRISPR; Transposon
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    130636-plasmid-map-sequence-id-255017

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  4. pSL0828 (pQCascade_crRNA-4)

    Plasmid
    #130637
    Purpose
    Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array encodes a guide RNA that targets lacZ.
    Depositor
    Samuel H. Sternberg
    Article
    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(target-4)
    Use
    CRISPR; Transposon
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    130637-plasmid-map-sequence-id-255021

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  7. pDG330

    Plasmid
    #100898
    Purpose
    SpCas9 with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CBh and U6
    Availability
    Academic Institutions and Nonprofits only
    100898-plasmid-map-sequence-id-195326

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  13. pFETCh_SSRP1

    Plasmid
    #72370
    Purpose
    Donor vector for 3' FLAG tag of human SSRP1
    Depositor
    Richard M. Myers, Eric Mendenhall
    Insert
    SSRP1 Homology Arms (SSRP1 Human)
    Use
    CRISPR
    Tags
    3XFLAG-P2A-NeoR
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    72370-plasmid-map-sequence-id-132120

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  14. pFETCh_TGIF2

    Plasmid
    #72373
    Purpose
    Donor vector for 3' FLAG tag of human TGIF2
    Depositor
    Richard M. Myers, Eric Mendenhall
    Insert
    TGIF2 Homology Arms (TGIF2 Human)
    Use
    CRISPR
    Tags
    3XFLAG-P2A-NeoR
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    72373-plasmid-map-sequence-id-132123

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  15. pX330-Flag-HeFSpCas9-H840A

    Plasmid
    #80456
    Purpose
    high fidelity enhanced SpCas9 mammalian expression vector
    Depositor
    Ervin Welker
    Insert
    HeFSpCas9_H840A
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Availability
    Academic Institutions and Nonprofits only
    80456-plasmid-map-sequence-id-151478

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  17. CROPseq-EFS-SpCas9-P2A-blast

    Plasmid
    #106477
    Purpose
    single-vector CROPseq system with blasticidin resistance selection for use in the CROPseq CRISPR knockout screens
    Depositor
    Alex Hewitt
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Availability
    Academic Institutions and Nonprofits only
    106477-plasmid-map-sequence-id-207983

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  18. GuideVector_BbsI

    Plasmid
    #110692
    Purpose
    For rapid cloning and expression of sgRNAs in mammalian cells. Contains copGFP for easy FACS sorting.
    Depositor
    Douglas Mahoney
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    110692-plasmid-map-sequence-id-211319

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  19. pTD56

    Plasmid
    #172895
    Purpose
    mKate2^SEC^AID* vector with ccdB markers for cloning homology arms
    Depositor
    David Reiner
    Insert
    mKate2
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    172895-plasmid-map-sequence-id-353627

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  20. pTD72

    Plasmid
    #172896
    Purpose
    mKate2^SEC^2xHA vector with ccdB markers for cloning homology arms
    Depositor
    David Reiner
    Insert
    mKate2
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    172896-plasmid-map-sequence-id-353630

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Showing: 1101 - 1120 of 20410 results