Showing: 1101 - 1120 of 20410 results
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Plasmid#203816PurposeExpression of CRISPR-associated transposases, shuttle vector for Corynebacterium glutamicum / E. coliDepositorInsertVchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(crtYf)
UseCRISPRTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCRISPathBrick
Plasmid#65006PurposeE. coli vector for expression of S. pyogenes dCas9, tracrRNA, and nontargeting CRISPR array with BsaI site for inserting user-defined spacer-repeat bricksDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterConstitutive native promotersAvailabilityAcademic Institutions and Nonprofits only -
pSL0762 (pQCascade_crRNA-nt)
Plasmid#130636PurposeExpresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array encodes a non-targeting guide RNA.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(non-targeting)
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pSL0828 (pQCascade_crRNA-4)
Plasmid#130637PurposeExpresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array encodes a guide RNA that targets lacZ.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(target-4)
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pBECKP-km
Plasmid#117235PurposeA cytidine deaminase-mediated base-editing plasmid in Klebsiella PneumoniaeDepositorInsertncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-SpyCas9
Plasmid#113034PurposeAAV vector for expression of SpyCas9 in mammalian cellsDepositorInsertSpyCas9
UseAAV, CRISPR, and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pDG330
Plasmid#100898PurposeSpCas9 with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.DepositorInsertshumanized CRISPR associated protein 9 Nickase
U6-gRNA scaffold 1
U6-gRNA scaffold 2
UseCRISPR and Mouse TargetingTags3xFLAGExpressionMammalianMutationPromoterCBh and U6AvailabilityAcademic Institutions and Nonprofits only -
pB[nos-Cas9 PUb-ZsGreen]
Plasmid#169011PurposeFor germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.DepositorInsertCas9
UsePiggybacTagsExpressionInsectMutationPromoterDrosophila melanogaster nanosAvailabilityAcademic Institutions and Nonprofits only -
pB[vasa-Cas9 PUb-ZsGreen]
Plasmid#169012PurposeFor germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.DepositorInsertCas9
UsePiggybacTagsExpressionInsectMutationPromoterDrosophila melanogaster vasaAvailabilityAcademic Institutions and Nonprofits only -
pFETCh_PBX2
Plasmid#72356PurposeDonor vector for 3' FLAG tag of human PBX2DepositorInsertPBX2 (PBX2 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pFETCh_ZNF146
Plasmid#72359PurposeDonor vector for 3' FLAG tag of human ZNF146DepositorInsertZNF146 Homology Arms (ZNF146 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pFETCh_ZNF219
Plasmid#72367PurposeDonor vector for 3' FLAG tag of human ZNF219DepositorInsertZNF219 Homology Arms (ZNF219 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pFETCh_SSRP1
Plasmid#72370PurposeDonor vector for 3' FLAG tag of human SSRP1DepositorInsertSSRP1 Homology Arms (SSRP1 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pFETCh_TGIF2
Plasmid#72373PurposeDonor vector for 3' FLAG tag of human TGIF2DepositorInsertTGIF2 Homology Arms (TGIF2 Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pX330-Flag-HeFSpCas9-H840A
Plasmid#80456Purposehigh fidelity enhanced SpCas9 mammalian expression vectorDepositorInsertHeFSpCas9_H840A
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationPromoterCBhAvailabilityAcademic Institutions and Nonprofits only -
U6-BbsI-crRNA
Plasmid#51026PurposeGenerates crRNA for use in combination with tracrRNADepositorInsertU6-BbsI-crRNA
UseCRISPRTagsExpressionInsectMutationPromoterU6-2AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-EFS-SpCas9-P2A-blast
Plasmid#106477Purposesingle-vector CROPseq system with blasticidin resistance selection for use in the CROPseq CRISPR knockout screensDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEFSAvailabilityAcademic Institutions and Nonprofits only -
GuideVector_BbsI
Plasmid#110692PurposeFor rapid cloning and expression of sgRNAs in mammalian cells. Contains copGFP for easy FACS sorting.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pTD56
Plasmid#172895PurposemKate2^SEC^AID* vector with ccdB markers for cloning homology armsDepositorInsertmKate2
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pTD72
Plasmid#172896PurposemKate2^SEC^2xHA vector with ccdB markers for cloning homology armsDepositorInsertmKate2
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 1101 - 1120 of 20410 results