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Plasmid#212928PurposeEncodes the mRuby2 fluorescent protein (Part3b') with the Aga2 signal peptide (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.DepositorInsertmRuby2
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRZ-mOct4-CNL
Plasmid#65718PurposemOct4 reporter plasmid (expresses CNL)DepositorInsertmurine Oct4 promoter and CNL
UseLentiviral and LuciferaseTagsExpressionMutationPromoterAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Tight-Puro_Nkx6.2
Plasmid#64846PurposeA lentiviral vector for the expression of mouse Nkx6.2 under doxycyline controlDepositorInsertNkx6.2 (Nkx6-2 Mouse)
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-Sa-SauriCas9
Plasmid#135967PurposeExpresses Sa-SauriCas9, and cloning backbone for sgRNADepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRZ-mNanog-YNL
Plasmid#65717PurposemNanog reporter plasmid (expresses YNL)DepositorInsertmurine Nanog promoter and YNL
UseLentiviral and LuciferaseTagsExpressionMutationPromoterAvailable sinceJuly 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
PB-Sox17
Plasmid#60669PurposePiggyBac expression vector encoding murine Sox17 downstream of a doxycycline inducible promoterDepositorInsertSox17 (Sox17 Mouse)
UsePiggybacTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
Cyan Nano-lantern/pcDNA3
Plasmid#67660Purposecyan luminescent protein for high-speed single-cell and whole body imagingDepositorInsertCyan Nano-lantern
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
Orange Nano-lantern/pcDNA3
Plasmid#67661Purposeorange luminescent protein for high-speed single-cell and whole body imagingDepositorInsertOrange Nano-lantern
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYNL-N1_TUBB5
Plasmid#65678PurposeExpresses beta-tubulin-YNL in mammalian cellsDepositorInsertbeta-tubulin class V
UseLuciferaseTagsYNLExpressionMammalianMutationPromoterCMVAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)::MBP-NL3F10H
Plasmid#141291PurposeMBP:NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)DepositorInsertMBP-NLUC3F10H
UseLuciferaseTagsFlag (x3), His (x10), and MBPExpressionBacterialMutationEngineered for high stability (t1/2 = 11.5 days a…Promoterpromoter for bacteriophage T7 RNA polymeraseAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBad-CA TAG126
Plasmid#105836PurposeExpresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 126 and C-terminal 6x his tagDepositorArticleInserthuman carbonic anhydrase II thermostable variant (CA2 Human)
UseTags6x his tagExpressionBacterialMutationTAG stop codon at site 126PromoterpBad/arabinoseAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
K8.1Pr-pGL4.16
Plasmid#131037PurposeFirefly Luciferase reporter for K8.1 promoter from KSHVDepositorInsertKSHV K8.1 100 bp promoter
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBEAST-pBen-sfGFP
Plasmid#114598Purpose"Output expression of sfGFP under the activation of BenR transcription factor for cell-free expression"DepositorInsertsfGFP
UseSynthetic Biology; Cell-free protein synthesisTagsExpressionMutationPromoterPBenAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBEAST-BenR
Plasmid#114597Purpose"Strong constitutive expression of transcription factor, BenR, for cell-free expression"DepositorInsertBenR
UseSynthetic Biology; Cell-free protein synthesisTagsExpressionMutationPromoterOR2-OR1-PrAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
Orange Nano-lantern/pRSETB
Plasmid#67659Purposeorange luminescent protein for high-speed single-cell and whole body imagingDepositorInsertOrange Nano-lantern
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cyan Nano-lantern/pRSETB
Plasmid#67658Purposecyan luminescent protein for high-speed single-cell and whole body imagingDepositorInsertCyan Nano-lantern
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBad-CA TAG20
Plasmid#105666Purposeexpresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 20 and C-terminal 6x his tagDepositorArticleInserthuman carbonic anhydrase II thermostable variant (CA2 Human)
UseTags6x his tagExpressionBacterialMutationTAG stop codon at site 20PromoterpBad/arabinoseAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
Cyan Nano-lantern(Ca2+)/pcDNA3
Plasmid#71344PurposeExpress Cyan Nano-lantern-based luminescent Ca2+ indicator in mammalian cellDepositorInsertCyan Nano-lantern(Ca2+)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMG343
Plasmid#176183Purposepreassembled yeast MoClo level-1 backbone with toxic gene dropout cassette (ccdB and TPK2) and URA3 markerDepositorInsertTPK2+ccdB dropout cassette
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKan-PCUP1-AID*(N)
Plasmid#99525PurposeN-terminal AID* degron cassette with CUP1 promoter and KanR marker for S. cerevisiaeDepositorInsertIAA17(71-117) (AXR3 Mustard Weed)
UsePcr template for yeast tagging cassetteTagsExpressionMutationPromoterCUP1Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only