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pIRIGF-IKZF3

Plasmid

#69046

Purpose

expression of IKZF3 fused to firefly luciferase with co-expression of Renilla luciferase, both under IRES control.

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF3 (IKZF3 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

IRES

Available since

Oct. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pYTK-DN4

Plasmid

#180285

Purpose

KanR multi-gene yeast expression vector (GFP-dropout)

Depositor

Andreas Milias-Argeitis

Article

Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

Insert

ConLS' - GFP - ConRE' - KanR - CEN6/ARS4

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pET-NDH6

Plasmid

#105503

Purpose

Expression of an N-terminally truncated form of Drosophila topoisomerase I with a C-terminal 6His tag

Depositor

James Kadonaga

Article

Khuong et al J Biol Chem. 2017 Nov 24;292(47):19478-1

Insert

NDH6

Use

Tags

6xHis

Expression

Mutation

Promoter

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pMP220

Plasmid

#185921

Purpose

Broad host range IncP promoter probe using lacZ

Depositor

Herman Spaink

Article

Spaink et al Plant Mol Biol. 1987 Jan;9(1):27-39. doi

Type

Empty backbone

Use

Incp

Tags

LacZ

Expression

Bacterial

Mutation

Promoter

None

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pAC103-PBNeo-U6-sgRNA-Expression

Plasmid

#48228

Purpose

sgRNA expression on PiggyBac vector with Neo-selectable marker

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 5, 2016

Availability

Academic Institutions and Nonprofits only

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MLRV3:HRE-P53-MAPK/JNK-FOXO-TGFβ

Plasmid

#178320

Purpose

Multiplex luciferase reporter vector, with luciferase reporters for the pathways HRE, P53, AP-1, FOXO and SMAD.

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

Inserts

HRE RedFirefly reporter
P53 FLuc reporter
AP-1 Renilla reporter
FOXO NLuc Reporter
SMAD GrRenilla reporter

Use

Luciferase and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV7.1_3xFLAG-WWC2

Plasmid

#172995

Purpose

constitutive expression of FLAG-tagged WWC2

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

3xFLAG-WWC2 (WWC2 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pYTK-DN3

Plasmid

#180284

Purpose

Spacer cassette (TU2)

Depositor

Andreas Milias-Argeitis

Article

Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

Insert

ConL1 - Spacer - ConR2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-LANP

Plasmid

#16175

Depositor

Huda Zoghbi

Article

Lim et al Cell. 2006 May 19. 125(4):801-14.

Insert

Acidic (leucine-rich) nuclear phosphoprotein 32 family, member A (ANP32A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pDU1

Plasmid

#169817

Purpose

pSpCas9(BB)-2A-tCD19 (pDU1)

Depositor

Andrea Rossi

Article

Ramachandran et al Life Sci Alliance. 2021 Apr 26;4(6). pii

Insert

tCD19

Use

Tags

fusion protein

Expression

Mammalian

Mutation

Promoter

Cbh

Available since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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