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Plasmid#79534PurposeExpresses C-terminally EGFP-tagged Arf5(WT) in mammalian cellsDepositorInsertArf5 (ARF5 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX FynT-SH2
Plasmid#46430DepositorInsertFynB/FynT (FYN Human)
UseTagsAviTag, GST, and PreScissionExpressionBacterialMutationcontains amino acids 142-251PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCR3-Flag-AuroraB KD (K106R)
Plasmid#108488Purposeexpression of Flag-AuroraB kinase death (K106R)DepositorInsertAuroraB (AURKB Human)
UseTagsFlagExpressionMammalianMutationKinase death K106APromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-RIα(D/D+linker)-EGFP
Plasmid#181842PurposeEGFP-tagged docking/dimerization (D/D) and linker region of PKA RIα; for mammalian expression.DepositorInsertRIα-(D/D+linker)EGFP (PRKAR1A Human)
UseTagsEGFPExpressionMammalianMutationContains only residues 12-112 of PKA RIα.PromoterCMVAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ITSN1-2/5
Plasmid#91385PurposeProtein expression and purification of human SH3 domain construct ITSN1-2/5DepositorInsertITSN1-2/5 (ITSN1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDF-casBCDE(g8)
Plasmid#89727PurposeExpresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing 33-nt spacer (referred to as 'wt') matching a protospacer in the M13 phage genome.DepositorInsertCRISPR array
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX Jak1-SH2
Plasmid#46447DepositorInsertJak1 (JAK1 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 430-552PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Abl-SH2 RK
Plasmid#46403DepositorInsertAbl RK (Abl1 Mouse)
UseTagsGST and Thrombin siteExpressionBacterialMutationcontains amino acids 139-239PromoterTacAvailable sinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDEST27-GST-ATXN1 FL(30Q)-S776D
Plasmid#21755DepositorInsertAtaxin-1 (ATXN1 Human)
UseTagsGSTExpressionMammalianMutationinsert contains a stretch of 32 amino acids (30 Q…PromoterCMVAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMCh2007_pLenti_Syn_mChe-cdc42E7
Plasmid#118620Purposeexpression of mChe-tagged cdc42E7 under pSyn promoter for primary neuronsDepositorInsertcdc42E7 (Cdc42 Mouse)
UseLentiviralTagsmCherryExpressionMammalianMutationchanged cagtctg to ggcataa (667 - 673 nt in 3…Promotersynapsin I (rat)Available sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PTBP1-RRM12CCtoSS
Plasmid#89155PurposeRecombinant expression of PTBP1-RRM12 C250S,C251S in E.coliDepositorInsertPolypyrimidine Tract Binding Protein 1 RNA Recognition Motif 1+2 mutant C250S, C251S (PTBP1 Human)
UseTagshexa HisExpressionBacterialMutationmutations: Cys 250 to Ser, Cys 251 to SerPromoterT7Available sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX Grb10-SH2
Plasmid#46437DepositorInsertGrb10 (GRB10 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 435-548PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HIS-Xyl-Xmod-DocIII
Plasmid#60865PurposeCharacteristic fingerprint molecule fused to dockerin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced Cystein in Xylanase T120CDepositorInsertsXyl
Xmod-DocIII
UseTags6xHISExpressionBacterialMutationThreonine 120 to Cysteine in Xylanase domainPromoterT7Available sinceNov. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_LASP1-1/1-SV1/2
Plasmid#91399PurposeProtein expression and purification of human SH3 domain construct LASP1-1/1-SV1/2DepositorInsertLASP1-1/1-SV1/2 (LASP1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX RASGAP(C)-SH2
Plasmid#46431DepositorInsertGap/RASGAP(C) (RASA1 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 339-452PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCS2plus-mVenus1-155-GGGS
Plasmid#162610PurposeAllows for transcription of control mVenus fluorophore half for injection into zebrafish embryos for BiFC assaysDepositorInsertmVenus
UseTagsExpressionMammalianMutationContains aa1-155 of mVenusPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
MO70-N-Flag-hCRACR2A-isoform-a-Q604L
Plasmid#79595PurposeTransient and retroviral expressionDepositorInsertCRACR2A-isoform-a (CRACR2A Human)
UseRetroviralTagsFLAGExpressionMammalianMutationQ604LPromoterCMVAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
MO70-N-Flag-hCRACR2A-isoform-a-T559N
Plasmid#79594PurposeTransient and retroviral expressionDepositorInsertCRACR2A-isoform-a (CRACR2A Human)
UseRetroviralTagsFLAGExpressionMammalianMutationT559NPromoterCMVAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
CRAF_S29A_pcDNA3.1
Plasmid#86506PurposeExpresses CRAF_S29A in mammalian cells [Edit]DepositorInsertCRAF (RAF1 Human)
UseTagsFLAGExpressionMammalianMutationS29APromoterCMVAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_CRK-2/2-SV2/2
Plasmid#91343PurposeProtein expression and purification of human SH3 domain construct CRK-2/2-SV2/2DepositorInsertCRK-2/2-SV2/2 (CRK Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only