Skip to main content
Addgene
Showing: 5581 - 5590 of 152174 results

  1. G protein alpha-s(alpha3beta5/G226A/A366S)

    Plasmid
    #55797
    Purpose
    This highly effective dominant negative G protein alpha-s mutant contains, in addition to the mutations in alpha-s(alpha3beta5/G226A), the A366S mutation, which increases GDP release.
    Depositor
    Catherine Berlot
    Article
    Berlot et al J Biol Chem. 2002 Jun 7;277(23):21080-5.
    Insert
    alpha-s (alpha3beta5/G226A/A366S) (Gnas Rat)
    Use
    Tags
    internal EE epitope (residues 189-194 in alpha-s …
    Expression
    Mammalian
    Mutation
    N271K, K274D, R280K, T284D, I285T, G226A, A366S i…
    Promoter
    CMV
    Available since
    Aug. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    55797-plasmid-map-sequence-id-112181

    Enlarge

  5. PB-TAC-ERP2

    Plasmid
    #80478
    Purpose
    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry and constitutive rtTA and puromycin resistance)
    Depositor
    Knut Woltjen
    Article
    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:
    Type
    Empty backbone
    Use
    Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    80478-plasmid-map-sequence-id-404034

    Enlarge

  9. pDG459

    Plasmid
    #100901
    Purpose
    SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-PuroR
    Expression
    Mammalian
    Mutation
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100901-plasmid-map-sequence-id-197197

    Enlarge

Showing: 5581 - 5590 of 152174 results