Addgene

We narrowed to 24,300 results for: sta

Showing: 11141 - 11160 of 24300 results

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Nanog-luciferase targeting construct

Plasmid

#26591

Depositor

Rudolf Jaenisch

Article

Lyssiotis et al Proc Natl Acad Sci U S A. 2009 Jun 2. 10

Insert

Nanog promoter-luciferase-lox-pgk-neomycin-loc

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pCA-T-intron(Neo)-G(LNL)

Plasmid

#36908

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

T (i.e., ATG, start codon as a first exon)
GFP
beta-globin intron
Neo

Use

Tags

Expression

Mammalian

Mutation

deleted nucleotides before nucleotide 275 and ins…

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only

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CKAP2-mNeonGreen pHAT

Plasmid

#175947

Purpose

Bacterial expression of mmCKAP2-mNeonGreen, N-terminal 6xHis-tag, C-terminal Strep-tagII

Depositor

Susanne Bechstedt

Article

McAlear et al Elife. 2022 Jan 14;11. pii: 72202. doi:

Insert

CKAP2 (Ckap2 Mouse)

Use

Tags

6His, StrepTagII, and mNeonGreen

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pMX-eSOX17WHC

Plasmid

#118839

Purpose

Retroviral expression of a mutant of Sox17 that improves efficiency of iPSC generation

Depositor

Ralf Jauch

Article

Veerapandian et al Stem Cell Reports. 2018 Aug 14;11(2):593

Insert

eSOX17WHC (Sox17 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

WHC: L46W, V53H, E57C

Promoter

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pMX-eSOX17FNV

Plasmid

#118838

Purpose

Retroviral expression of a mutant of Sox17 that improves efficiency of iPSC generation

Depositor

Ralf Jauch

Article

Veerapandian et al Stem Cell Reports. 2018 Aug 14;11(2):593

Insert

eSOX17FNV (Sox17 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

FNV: L46F, V53N, E57V

Promoter

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pETNKI-strepII-3C-LIC-kan

Plasmid

#108706

Purpose

Expression of a StrepII-tagged target protein with 3C protease cleavage site.

Depositor

Titia Sixma

Article

Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

Type

Empty backbone

Use

Tags

StrepII-3C protease cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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Sema5a-AP-His

Plasmid

#72035

Purpose

Expresses the extracellular region of the Sema5A protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema5a (Sema5a Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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E1010m (RFP)_CD

Plasmid

#66033

Purpose

MoClo Basic Part: CDS - Fluorescent protein. Red. Modified from Bba_E1010 to fix illegal sites. [C:E1010m:D]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Fluorescent reporter - RFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR/U6 HDAC4 shRNA

Plasmid

#32220

Depositor

Reuben Shaw

Article

Mihaylova et al Cell. 2011 May 13;145(4):607-21.

Insert

shRNA against murine HDAC4 (Hdac4 Mouse)

Use

RNAi; Gateway u6 entry vector

Tags

Expression

Mutation

Promoter

human U6

Available since

Sept. 16, 2011

Availability

Academic Institutions and Nonprofits only
Sema4b-Fc-His

Plasmid

#72155

Purpose

Expresses the extracellular region of the Sema4B protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema4b (Sema4b Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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Nrp1-AP-His

Plasmid

#71971

Purpose

Expresses the extracellular region of the Neuropilin 1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Nrp1 (Nrp1 Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS3_K227/230R

Plasmid

#127135

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS3 (RPS3 Human)

Use

Lentiviral

Tags

Expression

Mutation

K227R/K230R

Promoter

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS3_K230R

Plasmid

#127134

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS3 (RPS3 Human)

Use

Lentiviral

Tags

Expression

Mutation

K230R

Promoter

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS3_K227R

Plasmid

#127133

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS3 (RPS3 Human)

Use

Lentiviral

Tags

Expression

Mutation

K227R

Promoter

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pVRa12_807

Plasmid

#49663

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF12_807

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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PTPN13_PDZ_2

Plasmid

#103920

Purpose

Protein expression and purification of PTN13 PDZ2 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

PTPN13 (PTPN13 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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PTPN13_PDZ_5

Plasmid

#103923

Purpose

Protein expression and purification of PTN13 PDZ5 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

PTPN13 (PTPN13 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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XE123 XDsh delta PDZ-GFP- CS2+

Plasmid

#16786

Depositor

Randall Moon

Article

Miller et al J Cell Biol. 1999 Jul 26;146(2):427-37.

Insert

Xdsh-delta-PDZ (dvl2.L Frog)

Use

Xenopus expression

Tags

gfp

Expression

Mutation

contains Dsh amino acids 1-267 and 381-736 (delet…

Promoter

Available since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
pEN_TT 3xFLAG-KPNA1

Plasmid

#192304

Purpose

Gateway entry vector for an inducible 3xFLAG-KPNA1

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

KPNA1 (KPNA1 Human)

Use

Gateway entry vector

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR/U6 HDAC5 shRNA

Plasmid

#32222

Depositor

Reuben Shaw

Article

Mihaylova et al Cell. 2011 May 13;145(4):607-21.

Insert

shRNA against mouse HDAC5 (Hdac5 Mouse)

Use

RNAi; Gateway u6 entry vector

Tags

Expression

Mutation

Promoter

human U6

Available since

Sept. 16, 2011

Availability

Academic Institutions and Nonprofits only