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pDEST-DHFR F[1,2]-C (TRP1)

Plasmid

#177795

Purpose

Gateway destination vector to insert genes of interest having a C-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

Type

Empty backbone

Use

Tags

DHFR F[1,2]

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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LV-miR-142

Plasmid

#117056

Purpose

Overexpression of miR-142-3p using miR-451 backbone to enable DICER-independent expression along with orange fluorescent protein (OFP) to track miRNA expression

Depositor

Michele De Palma

Article

Baer et al Nat Cell Biol. 2016 Jul;18(7):790-802. d

Insert

miR-142-3p - OFP (Mir142 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV promoter

Available since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-Cep70

Plasmid

#136821

Purpose

Mammalian expression of the centrosomal protein Cep70 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Cep70 (CEP70 Synthetic, Human)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pNOC_hfnCas12a_NR1_NR2

Plasmid

#176252

Purpose

pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and CRISPR array with spacers 1 and 2 targeting the Nitrate reductase gene of N. oceanica IMET1 separated by full

Depositor

John van der Oost

Article

Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

Insert

humanized fnCas12a

Use

CRISPR, Luciferase, and Synthetic Biology ; Expre…

Tags

luciferase

Expression

Mutation

Promoter

Available since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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[UBC][EIF4e][GG cloning]

Plasmid

#97189

Purpose

EIF4e plasmid with Pum cloning site

Depositor

Edward Boyden

Article

Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

Insert

EIF4E variant 1 (EIF4E Human)

Use

Synthetic Biology

Tags

empty Pum cloning site

Expression

Mammalian

Mutation

Promoter

UBC (ubiquitin C)

Available since

Nov. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pHH0103_ARHGEF6-1/1

Plasmid

#91515

Purpose

Protein expression and purification of human SH3 domain construct ARHGEF6-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ARHGEF6-1/1 (ARHGEF6 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTT3-SP-6xHis-KIR2DL5(FL)-FLAG

Plasmid

#157623

Purpose

Mammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assays

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

KIR2DL5 (KIR2DL5A Human)

Use

Tags

6xHis and FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-INTS3-C

Plasmid

#128417

Purpose

Express INTS3 C-termini (514-1042 aa) in E. coli with a GST tag (N-terminal)

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

Insert

INTS3 (INTS3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

INTS3 C-terminal region (514-1042 aa)

Promoter

Tac promoter

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-MBP-Foldon-ATG9 (692-839)

Plasmid

#188087

Purpose

To make ATG9 C-terminal tail trimer for expression in mammalian cells

Depositor

James Hurley

Article

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG9 (ATG9A Human)

Use

Tags

MBP tag

Expression

Mammalian

Mutation

ATG9 (692-839)

Promoter

CMV

Available since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST27-GST-ATXN1 FL(92Q)-S776D

Plasmid

#21757

Depositor

Huda Zoghbi

Article

Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

Insert

Ataxin-1 (ATXN1 Human)

Use

Tags

GST

Expression

Mammalian

Mutation

insert contains a stretch of 92 Q(Glutamines) and…

Promoter

CMV

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
pTriEx4-ADCY6-C2 (917-1165 aa)

Plasmid

#113904

Purpose

His-S tagged cytoplasmic catalytic domain 2 of Adenylate Cyclase 6

Depositor

Anjaparavanda Naren

Article

Thomas et al J Biol Chem. 2018 Jun 14. pii: RA118.003

Insert

Adenylate Cyclase 6 cytoplasmic catalytic domain 2 (ADCY6 Human)

Use

Tags

His-S

Expression

Bacterial, Insect, and Mamm…

Mutation

Please see Depositor Comments

Promoter

T7, CMV

Available since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pTriEx4-ADCY6-C1(306-672 aa)

Plasmid

#113903

Purpose

His-S tagged cytoplasmic catalytic domain 1 of Adenylate Cyclase 6

Depositor

Anjaparavanda Naren

Article

Thomas et al J Biol Chem. 2018 Jun 14. pii: RA118.003

Insert

Adenylate Cyclase 6 cytoplasmic catalytic domain 1 (ADCY6 Human)

Use

Tags

His-S

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

T7, CMV

Available since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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hRAD18 dC2-EGFP

Plasmid

#68826

Purpose

Expresses human RAD18 (deleting Polymerase eta binding domain) tagged with EGFP

Depositor

Satoshi Tateishi

Article

Tsuji et al Genes Cells. 2008 Apr;13(4):343-54. doi:

Insert

human RAD18 (deleting Polymerase eta binding domain) tagged with EGFP (RAD18 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA-FRT-TO-FH-Nsp7-Nsp8_GC3opt

Plasmid

#157692

Purpose

mammalian expression of N-terminally Flag-His8 tagged Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp7-Nsp8_GC3opt (ORF1ab SARS-CoV-2)

Use

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized; gene expression was optimized by…

Promoter

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-CMV-dSa VP64 Actc1

Plasmid

#99691

Purpose

Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Actc1 promoter, vector allows for activation of mouse Actc1, can be packaged and delivered as AAV

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9 and gRNA targeting Actc1 (gRNA sequence: GCCATTCTTGGAGCCAAGGG) (Actc1 Synthetic, Mouse)

Use

AAV and CRISPR

Tags

VP64

Expression

Mutation

dead Cas9

Promoter

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pEGB 35S:dCas9:Tnos (GB1191)

Plasmid

#68223

Purpose

Transcriptional unit for human codon optimized with mutated (D10A, H840A) and inactivated catalytic domains Cas9 protein plant expression driven by the 35S promoter

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

dCas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI and BsmBI sites removed; human codon optimis…

Promoter

35S

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pEBTetBl-CLIP-Cep78

Plasmid

#136876

Purpose

Mammalian expression of the centrosomal protein Cep78 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-Cep78 (CEP78 Synthetic, Human)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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HOXB3 (human) FLAG pSP65

Plasmid

#8523

Depositor

Corey Largman

Article

Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

Insert

HOXB3 (HOXB3 Human)

Use

In vitro transcription/translation

Tags

FLAG

Expression

Bacterial

Mutation

Contains 5 additional amino acids before ATG from…

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pLR5-CAG-NLS-tdiRFP670-2A-LBR

Plasmid

#82620

Purpose

Expresses NLS-tdiRFP-2A-LBR in mammalian cells

Depositor

Hiroshi Ochiai

Article

Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

Insert

NLS-tdiRFP670-LBR (Lbr Synthetic, Mouse)

Use

Piggybac vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pEBTetDBl-CLIP-α-tubulin

Plasmid

#136841

Purpose

Mammalian expression of the centrosomal protein _-tubulin N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-TUBA (DNMBP Synthetic, Human)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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