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Plasmid#217819PurposeExpresses DaPFOR in E. coliDepositorInsertpACYCDUET-StrepSUMO-DaPFOR
UseTagsStrepSUMOExpressionBacterialMutationPromoterT7Available sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/t35s[TMV]:NLuc-I{AGO-405}:NosT
Plasmid#212182PurposeThis binary vector expresses Nanoluciferase (Nluc) containing the Argonaut (AGO) intron (405 bp into the gene) with the truncated 35sCAMV promoter and the tomato mosaic virus UTRDepositorInsertNanoLuc luciferase-I{AGO-405}
UseTagsExpressionPlantMutationAGO intron added at bp 405, eliminates expression…Promotertruncated 35sCaMVAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/t35s[TMV]:mNeonGreen-I{PIV-198}:NosT
Plasmid#212166PurposeThis binary vector expresses mNeonGreen containing the PIV intron (198bp into the gene) with the truncated 35sCAMV promoter and the tomato mosaic virus UTRDepositorInsertTomato codon optimized version of mNeonGreen-I{PIV-198}
UseTagsExpressionPlantMutationmNeonGreen codon-optimized for tomato and PIV int…Promotertruncated 35sCaMVAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/t35s[TMV]:mNeonGreen-I{PIV-216}:NosT
Plasmid#212167PurposeThis binary vector expresses mNeonGreen containing the PIV intron (216bp into the gene) with the truncated 35sCAMV promoter and the tomato mosaic virus UTRDepositorInsertTomato codon optimized version of mNeonGreen-I{PIV-216}
UseTagsExpressionPlantMutationmNeonGreen codon-optimized for tomato and PIV int…Promotertruncated 35sCaMVAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/t35s[TMV]:mNeonGreen-I{PIV-390}:NosT
Plasmid#212168PurposeThis binary vector expresses mNeonGreen containing the PIV intron (390bp into the gene) with the truncated 35sCAMV promoter and the tomato mosaic virus UTRDepositorInsertTomato codon optimized version of mNeonGreen-I{PIV-390}
UseTagsExpressionPlantMutationmNeonGreen codon-optimized for tomato and PIV int…Promotertruncated 35sCaMVAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-C17orf53_1-87-mCer
Plasmid#211441Purposemammalian expression of the N-terminal amino acids 1-87 of human C17orf53 C-terminally fused to mCeruleanDepositorInsertC17orf53 (HROB Human)
UseTagsmCeruleanExpressionMammalianMutationDeletion of aa 88-646PromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-C17orf53_1-87-mVen
Plasmid#211442Purposemammalian expression of the N-terminal amino acids 1-87 of human C17orf53 C-terminally fused to mVenusDepositorInsertC17orf53 (HROB Human)
UseTagsmVenusExpressionMammalianMutationDeletion of aa 88-646PromoterAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAS_MmaPylT_EF1_NES-MmaPylRS WT
Plasmid#174901Purposeexpression of Mma PylRS with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integrationDepositorInsertMma PylRS (pylS Methanosarcina mazei)
UseTagsFLAG and NESExpressionMammalianMutationPromoterEF1Available sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET24a MDH_HALMA
Plasmid#204241PurposeBacterial expression of H. marismortui (Dead Sea halophilic red archaeon) cytosolic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDH_HALMA (mdh Haloarcula marismortui)
UseTags6X HisExpressionBacterialMutationPromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsTNRC6C_1248-1600_I
Plasmid#146590PurposeMammalian Expression of HsTNRC6C_1248-1600DepositorInsertHsTNRC6C_1248-1600 (TNRC6C Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsTNRC6Biso1_1250-1723_H
Plasmid#146510PurposeMammalian Expression of HsTNRC6Biso1_1250-1723DepositorInsertHsTNRC6Biso1_1250-1723 (TNRC6B Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsTNRC6C_1248-1690_H
Plasmid#146512PurposeMammalian Expression of HsTNRC6C_1248-1690DepositorInsertHsTNRC6C_1248-1690 (TNRC6C Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsTNRC6C-MID2_H
Plasmid#146517PurposeMammalian Expression of HsTNRC6C-MID2DepositorInsertHsTNRC6C-MID2 (TNRC6C Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsTNRC6Biso1_1-1256_H
Plasmid#146509PurposeMammalian Expression of HsTNRC6Biso1_1-1256DepositorInsertHsTNRC6Biso1_1-1256 (TNRC6B Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHS33
Plasmid#171202Purposebacterial experssion of a GST-tagged MEG-3 C-terminus with mutations in the HMG-like motifDepositorInsertmeg-3 (meg-3 Nematode)
UseTagsGSTExpressionBacterialMutationaa545-862, F705A,Y708A,Y713A, N725APromoterAvailable sinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAP21
Plasmid#171207Purposebacterial expression for HIS purification of the MEG-3 C-terminus with mutations in the HMG-like motifDepositorInsertmeg-3 (meg-3 Nematode)
UseTags8xHISExpressionBacterialMutationaa545-862, F705A,Y708A,Y713A, N725APromoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330.orup
Plasmid#110404PurposeCRISPR/Cas9 vector with sgRNA expression and puromycin resistanceDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterCBhAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
lvl0 NOSp
Plasmid#153375PurposePlant promoterDepositorInsertNOS promoter
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
lvl0 APT1p
Plasmid#153372PurposePlant promoterDepositorInsertAPT1 promoter
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
lvl0 35Sp
Plasmid#153369PurposeCauliflower mosaic virus promoter for plant expressionDepositorInsert35S promoter
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only