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Plasmid#19850DepositorInsertMKL1 L272A (MRTFA Human)
UseTags3xFLAGExpressionMammalianMutationchanged Leucine 272 to AlaninePromoterAvailable sinceMarch 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCin4-3xFLAG-MKL1 S449A
Plasmid#19842DepositorInsertMKL1 S449A (MRTFA Human)
UseTags3xFLAGExpressionMammalianMutationchanged Serine 449 to AlaninePromoterAvailable sinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 CALR
Plasmid#67086PurposeStandard for cell-free expression benchmarking.DepositorInsertCALR (CALR Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
SL3-MS2-gRNA GG backbone (MOBE3-4)
Plasmid#219949PurposeGolden gate backbone for mammalian expression of Sp-gRNA with SL3-MS2DepositorInsertgRNA-MS2-SL3 (S. pyogenes Cas9)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
3'-com-gRNA GG backbone (MOBE1-4)
Plasmid#219947PurposeGolden gate backbone for mammalian expression of Sp-gRNA with 3'-comDepositorInsertgRNA-com-3'end (S. pyogenes Cas9)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
3'MS2-gRNA GG backbone (MOBE1-2)
Plasmid#219948PurposeGolden gate backbone for mammalian expression of Sp-gRNA with 3'-MS2DepositorInsertgRNA-MS2-3'end (S. pyogenes Cas9)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
NK621
Plasmid#196405PurposeStrong expression of firefly luciferase codon-optimized for S. rosettaDepositorInsertSro5actin-firefly-Sro3actin
UseLuciferaseTagsExpressionMutationN/A (synthetic)PromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
NK809
Plasmid#196406PurposeStrong expression of firefly and nanoluc luciferases codon-optimized for S. rosettaDepositorInsertSro5EFL-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
UseLuciferaseTagsExpressionMutationN/A (synthetic)PromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
NK810
Plasmid#196407PurposeNanoluc luciferase under S. rosetta SPAG6 promoter with internal actin-firefly controlDepositorInsertSro5spag6-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
UseLuciferaseTagsExpressionMutationN/A (synthetic)PromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGas-miRFP718nano
Plasmid#195752PurposeG protein alpha-subunit internally tagged with miRFP718nanoDepositorInsertG protein alpha-s-miRFP718nano
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCytERM-miRFP670nano3
Plasmid#184674PurposeSignal anchor of rabbit cytochrome p450 and miRFP670nano3DepositorInsertCytERM-miRFP670nano3
UseTagsmiRFP670nano3ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_HSPA8-Halo
Plasmid#175325PurposeProduction of HaloTag fusion proteinDepositorInsertHSPA8 (HSPA8 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_Sce-Killer Protein SP
Plasmid#153498PurposeS. cerevisiae Killer Signal Peptide Donor PlasmidDepositorInsertKiller Protein SP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_Sce-Invertase 2 SP
Plasmid#153493PurposeS. cerevisiae Invertase Signal Peptide Donor PlasmidDepositorInsertInvertase 2 SP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_Sce-Acid Phosphatase SP
Plasmid#153494PurposeS. cerevisiae Acid Phosphatase Signal Peptide Donor PlasmidDepositorInsertAcid Phosphatase SP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_LynNanobody IRES EGFP
Plasmid#136624PurposePlasmid encoding outer inner membrane localization of the Enhancer nanobody GFP as a SNAP-Tag-fusion and EGFPDepositorInsertLyn11-SNAP-Tag-EnhancerNb (IRES EGFP)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCuHA-SpAtg17(416)
Plasmid#122079PurposeExpressing S. pombe HA-Atg17DepositorInsertSpATG17 (atg17 Fission Yeast)
UseTags3HAExpressionYeastMutationPromoterAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCu-SpAtg17(416)
Plasmid#122078PurposeExpressing S. pombe Atg17DepositorInsertSpATG17 (atg17 Fission Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-32
Plasmid#120011PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with S-TagDepositorInsertS-Tag
UsePcr templateTagsS-TagExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
PDXK_pCSdest
Plasmid#53900DepositorInsertPDXK (PDXK Human)
UseTagsExpressionMammalianMutationPromoterSP6 and CMVAvailable sinceMarch 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits