Addgene

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SITS-ccdb-EGFP

Plasmid

#118752

Purpose

Multisite gateway destination vector for 3' tagging with EGFP for cell free expression. Parton lab FEX

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ccdb

Use

Tags

EGFP

Expression

Mutation

Promoter

Available since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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SITS-mCherry-ccdb

Plasmid

#118629

Purpose

Multisite gateway destination vector for 5' tagging with mCherry for cell free expression. Parton lab clone FKZ

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ccdb

Use

Tags

mCherry

Expression

Mutation

Promoter

Available since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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SITS-EGFP-ccdb

Plasmid

#118628

Purpose

Multisite gateway destination vector for 5' tagging with EGFP for cell free expression. Parton lab clone FEZ

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ccdb

Use

Tags

EGFP

Expression

Mutation

Promoter

Available since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_DL121_WTTS

Plasmid

#171954

Purpose

Selection vector for chimeric fusion of ecDHFR and asLOV2.

Depositor

Kimberly Reynolds

Article

McCormick et al Elife. 2021 Jun 16;10. pii: 68346. doi:

Inserts

Fusion of ecDHFR and asLOV2 at position 121 of DHFR
Thymidylate Synthase

Use

Tags

8-His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pBD21_035

Plasmid

#109152

Purpose

pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of EGFP

Depositor

Ron Weiss

Article

Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1

Inserts

mRFP
EGFP

Use

Synthetic Biology

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV and pTet

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pTJV1ScKm-rpoB

Plasmid

#166980

Purpose

Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; npt2 for kanamycin resistance

Depositor

Christopher Reisch

Article

Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

Insert

NeoR/KanR

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pdCas9_CL

Plasmid

#166245

Purpose

regulated dCas9 generator

Depositor

Domitilla Del Vecchio

Article

Huang et al Nat Commun. 2021 Mar 16;12(1):1692. doi:

Insert

P112-sgRNA-term-P104-RBS1-dCas9-B15

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Ec-TTL-P112 and Ec-TTL-P104

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pAUX_OL

Plasmid

#166246

Purpose

use as an auxiliary temperature-sensitive plasmid to successfully transform pdCas9_CL plasmid

Depositor

Domitilla Del Vecchio

Article

Huang et al Nat Commun. 2021 Mar 16;12(1):1692. doi:

Insert

[P112-sgRNA-term]-[J23116_B34-dCas9-B15]

Use

Tags

Expression

Bacterial

Mutation

Promoter

Ec-TTL-P112 and BBa_J23116

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCTCON2-GXB

Plasmid

#160926

Purpose

Dual fluorescent protein reporter (GFP-BFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Inserts

BFP
sfGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1-10

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pFapO_94

Plasmid

#149597

Purpose

pXI-3-LoxP-KlURA3-pTEF1- FapO_94-yeGFP

Depositor

Michael Jensen

Article

Ambri et al ACS Synth Biol. 2020 Feb 21;9(2):218-226

Insert

yeGFP

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

TEF1-FapO_94

Available since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pXylO_94

Plasmid

#149598

Purpose

pXI - 3 -LoxP -KlURA3 -pTEF1 - XylO_94 -yeGFP

Depositor

Michael Jensen

Article

Ambri et al ACS Synth Biol. 2020 Feb 21;9(2):218-226

Insert

yeGFP

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

TEF1-XylO_94

Available since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pTetO_105

Plasmid

#149599

Purpose

pXI - 3 -LoxP -KlURA3 -pTEF1 - TetO_105 -yeGFP

Depositor

Michael Jensen

Article

Ambri et al ACS Synth Biol. 2020 Feb 21;9(2):218-226

Insert

yeGFP

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

TEF1-TetO_105

Available since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pFA271

Plasmid

#149600

Purpose

(parent plasmid pCFB262) pXII - 4 -LoxP -SpHIS5 -209bp - pCYC1_BenO_183 -yeGFP

Depositor

Michael Jensen

Article

Ambri et al ACS Synth Biol. 2020 Feb 21;9(2):218-226

Insert

yeGFP

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pFA272

Plasmid

#149601

Purpose

(parent plasmid pCFB262) pXII - 4 -LoxP -SpHIS5 -209bp - pCYC1_BenO_182 -yeGFP

Depositor

Michael Jensen

Article

Ambri et al ACS Synth Biol. 2020 Feb 21;9(2):218-226

Insert

yeGFP

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pFA197

Plasmid

#149602

Purpose

(parent plasmid pCFB262) pXII - 4 -LoxP -SpHIS5 -209bp - pCYC1 -PcaO -108 -yeGFP

Depositor

Michael Jensen

Article

Ambri et al ACS Synth Biol. 2020 Feb 21;9(2):218-226

Insert

yeGFP

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

PcaO_1O8

Available since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pVD_79

Plasmid

#149603

Purpose

(parent plasmid pCFB257) pX - 3 -LoxP -KlLEU2 -pTEF1 - TetR

Depositor

Michael Jensen

Article

Ambri et al ACS Synth Biol. 2020 Feb 21;9(2):218-226

Insert

TetR

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Available since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pEE22

Plasmid

#161787

Purpose

Hi-SC2 reporter delivery plasmid

Depositor

François-Xavier Barre

Article

Espinosa et al Mol Cell. 2020 Sep 3;79(5):857-869.e3. d

Insert

Hi-SC2 reporter

Use

Conjugative plasmid to deliver hi-sc2 reporter

Tags

Expression

Mutation

Himar site included MmeI site, tandem loxP sites,…

Promoter

n/a

Available since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pCL66-decoder

Plasmid

#159453

Purpose

Insert: Pfus1mut-tetR-nls-malE-tCyc1. Same insert as pCL33-decoder in a multiple integration shuttle vector. Backbone: pRG235 (addgene). Marker: Leu2.

Depositor

Joerg Stelling

Article

Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:

Insert

Pfus1mut-tetR-nls-malE-tCyc1

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Pfus1mut

Available since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pVD_01

Plasmid

#149606

Purpose

pTEF1-VanR inserted in pRS416 backbone

Depositor

Michael Jensen

Article

Ambri et al ACS Synth Biol. 2020 Feb 21;9(2):218-226

Insert

VanR

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Available since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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907F1

Plasmid

#160233

Purpose

FlightN-VanR-VP16-3X-SV40-attB

Depositor

Omar Akbari

Article

Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

Inserts

VanR
Flightin promoter

Use

Tags

VP16

Expression

Insect

Mutation

Promoter

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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