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Plasmid#73625PurposeProduces Escherichia coli adenylate kinase (PlsA), N-terminal His6 tagDepositorInsertadenylate kinase (adk Escherichia coli str. K-12 substr. MG1655)
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBx1-VHH template3-3XMyc-Spacer
Plasmid#176210PurposePCR template for generating CeVICA VHH/nanobody libraryDepositorInsertVHH template3-3XMyc-Spacer
UseTagsMyc tagExpressionBacterialMutationPromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBx1-VHH template2-3XMyc-Spacer
Plasmid#176209PurposePCR template for generating CeVICA VHH/nanobody libraryDepositorInsertVHH template2-3XMyc-Spacer
UseTagsMyc tagExpressionBacterialMutationPromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-2xFLAG-Atoh1
Plasmid#101734PurposeExpresses mouse Atoh1 with FLAG tagDepositorInsertAtoh1 (Atoh1 Mouse)
UseTags2x FLAGExpressionMammalianMutationPromoterAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_ERBB2
Plasmid#183287PurposeAll-in-One CRISPRko system with a guide RNA that targets ERBB2 geneDepositorInsertERBB2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2359 - intron GG2 - 250bp
Plasmid#159878PurposeGolden Gate compatible intron with PATCs for reduced germline silencingDepositorInsertintron GG2 - 250bp
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCVL SFFV HA.NLS.I-AniIY2(reo).T2A.TagBFP
Plasmid#31477DepositorInsertSFFV HA NLS Y2 T2A mTagBFP
UseLentiviralTagsHA and NLSExpressionMutationF13Y + S111Y on I-AniI = Y2 "reo" (res…PromoterAvailable sinceAug. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET15b+PCNA1 P186C
Plasmid#66173PurposeExpresses the P186C mutant of Sulfolobus solfataricus PCNA1DepositorInsertDNA polymerase sliding clamp 1
UseTagsHis6 tagExpressionBacterialMutationP186CPromoterT7 promoterAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2369 - intron GG1 - 250bp
Plasmid#159877PurposeGolden Gate compatible intron with PATCs for reduced germline silencingDepositorInsertintron GG1 - 250bp
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMXs-PT2A-GFP-iRFP670-Tdtomato
Plasmid#111813PurposeTricistronic construct: Co-express three fluorescent proteins by P2A and then T2ADepositorInsertsGFP
iRFP670
Tdtomato
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDM1489
Plasmid#108996PurposeCre expression vectorDepositorInsertCre recombinase
UseCre/Lox; Cre expression vectorTagsExpressionMutationPromoteract15Available sinceJuly 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-GCaMP6f
Plasmid#166022PurposeExpresses GCaMP6f in CaMKII+ cellsDepositorInsertGCaMP6f
UseAAV and Mouse TargetingTagsNoneExpressionMammalianMutationPromoterAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSiMPlc_N + pSiMPlc_C
Plasmid#134312PurposeMaintenance of 2 plasmids with a single antibiotic in E. coliDepositorInsertcmR_gp41-1 N-intein + cmR_gp41-1 C-intein
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXJ40-Flag-Ferritin-iBox-PAK4cat
Plasmid#190180Purposeexpresses Flag-tagged Ferritin-iBox-PAK4catDepositorInsertFerritin-iBox-PAK4cat
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZG61
Plasmid#202575PurposeRNA editing in plants. Plasmid contains 35S-dADARcd-LIC1-ccdB-LIC2-35S-hptIIDepositorInsertHyperADARcd
UseTagsExpressionPlantMutationE488QPromoterAvailable sinceDec. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZG71
Plasmid#202577PurposeRNA editing in plants. Plasmid contains 35S-dADARcd-LIC1-ccdB-LIC2-dADARcd-35S-hptIIDepositorInsertHyperADARcd
UseTagsExpressionPlantMutationE488QPromoterAvailable sinceDec. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
rAAV-syn::FLEX-rev::PSAML141F:GlyR-IRES-GFP
Plasmid#32479DepositorInsertPSAML141F-GlyR-IRES-GFP
UseAAV and Cre/Lox; Adeno-associated virus, flex swi…TagsIRES-EGFPExpressionMutationPromoterSynapsinAvailable sinceJan. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDM1488
Plasmid#108995PurposeCre expression vectorDepositorInsertCre recombinase
UseCre/Lox; Cre expression vectorTagsExpressionMutationPromoteract15Available sinceJuly 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCI108-SiT-Cas12a-[Cond]
Plasmid#128407PurposeConditionally express both AsCas12a and CRISPR arrays on single transcriptsDepositorInsertAsCas12a-Triplex
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAtCas9_3
Plasmid#91158PurposeCas9 only expression, Cas9 Type: AtCas9, Plant Selection: 2x35S:barDepositorInsertAtCas9
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only