Addgene

We narrowed to 20,487 results for: crispr

Showing: 11121 - 11140 of 20487 results

Results per page:

Sort results by:

pCAG-T3-eSpCAS9-NG-pA

Plasmid

#131466

Purpose

Expresses eSpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroys

Depositor

Wataru Fujii

Article

Fujii et al Sci Rep. 2019 Sep 9;9(1):12878. doi: 10.

Insert

Codon optimized eSpCas9-NG

Use

CRISPR

Tags

Flag, NLS, and polyA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAGM55261

Plasmid

#153210

Purpose

Level 2 cloning vector for a single guide RNA, already contains Cas9, Bar selection cassette and FAST marker

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-SpRY-ABE8e

Plasmid

#185671

Purpose

Expresses SpRY-ABE8e in mammalian cells

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

ecTadA(8e)-nSpRY

Use

CRISPR

Tags

Expression

Mammalian

Mutation

SpRY D10A

Promoter

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
BA31_pU.CAG.SaCas9-D10A.rBGpA

Plasmid

#199251

Purpose

Expression construct encoding the S. aureus Cas9.D10A nickase (SaCas9.D10A)

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

Insert

SaCas9.D10A nickase

Use

CRISPR

Tags

3XHA epitope, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Mutation

D10A

Promoter

CAG promoter

Available since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC57-CmYLCV

Plasmid

#205245

Purpose

For plant prime editing in wheat plants or monocotyledons protoplasts

Depositor

Zong Yuan

Article

Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

Insert

CmYLCV-csy4_binding_site-esgRNA-tevopreQ1-csy4_binding_site

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

CmYLCV

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pmCherry_gRNA

Plasmid

#80457

Purpose

gRNA expression vector with mCherry transfection control

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Inserts

sp Cas9 gRNA
mCherry

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 and chicken β-actin promoter

Available since

Aug. 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-gRNA-Puro

Plasmid

#121121

Purpose

Expression of a gRNA together with a Puromycin resistance

Depositor

Pablo Navarro

Article

Heurtier et al Nat Commun. 2019 Mar 7;10(1):1109. doi:

Insert

gRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458-mcherry

Plasmid

#161974

Purpose

Expresses Cas9-p2a-mcherry (BbsI site silenced) and sgRNA

Depositor

Joanna Wysocka

Article

Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

Insert

Cas9-t2a-mcherry

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Cbh

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
L4440_BioBrick-sgRNA

Plasmid

#177783

Purpose

Overexpression of sgRNAs in E. coli HT115, sgRNA overexpression scaffold; for ingestion by C. elegans

Depositor

Michael Ristow

Article

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE

Plasmid

#60910

Purpose

dCas9 fused to 24 copies of the GCN4 peptide v4, which is part of the SunTag system

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

2x NLS, HA, and NLS

Expression

Mammalian

Mutation

Promoter

dSV40 Promoter

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCas9-GFP

Plasmid

#78546

Purpose

Fusion protein Cas9+GFP

Depositor

Rory Johnson, Roderic Guigo

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

GFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Fusion protein with Cas9

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pICSL01009::AtU6p

Plasmid

#46968

Purpose

Encodes the Arabidopsis U6 promoter in a level 0 vector (Weber et al. PLoS One 6:e16765, 2011). It is used to place an sgRNA uder the Arabidopsis U6 promoter.

Depositor

Sophien Kamoun

Article

Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

Insert

AtU6p

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCas9

Plasmid

#106396

Purpose

Recombineering plasmid with constitutively expressed cas9 and the araBAD promoter expressing αβγ

Depositor

Brian Pfleger

Article

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Inserts

cas9
Lambda red genes

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23105 and araBAD

Available since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-EFS-SpCas9

Plasmid

#104588

Purpose

AAV vector expressing Myc-tagged SpCas9 under the EFS promoter

Depositor

Ryohei Yasuda

Article

Nishiyama et al Neuron. 2017 Oct 18. pii: S0896-6273(17)

Insert

Streptococcus pyogenes Cas9

Use

AAV and CRISPR

Tags

Myc

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pXPR_502_sgCD45

Plasmid

#158705

Purpose

CD45 CRISPRa control

Depositor

John Doench, David Root

Article

Orchard et al J Virol. 2018 Dec 10;93(1). pii: JVI.013

Insert

CD45 (PTPRC Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pVEGFR1_TEV(C)_TCS(Q'G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

Plasmid

#101103

Purpose

Encodes dCas9(C)-VP64 with SAM components fused to VEGFR1

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

VEGFR1-dCas9(C)

Use

CRISPR and Synthetic Biology

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pUCC001

Plasmid

#124451

Purpose

Multi-species CRISPR/Cas9 coexpression system, optimized for Golden Gate cloning of gRNA targets

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

HH-BsaI

Use

Tags

Expression

Yeast

Mutation

gRNA expression cassette from pUDP002 modified to…

Promoter

TDH3 promoter from S.cerevisiae (already present …

Available since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCB-1

Plasmid

#210387

Purpose

All-in-one cumate-based inducible CRISPRi plasmid for use in Streptomyces

Depositor

Gilles van Wezel

Article

Bai et al ACS Synth Biol. 2023 Oct 6. doi: 10.1021

Inserts

dCas9
CymR
sgRNA cassette

Use

CRISPR; Integrative vector for use in streptomyces

Tags

Expression

Mutation

BsaI-flanked RFP cassette inserted in place of sp…

Promoter

SP11 and SP30

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
U6-AsCas12f-full-length-sgRNA

Plasmid

#204638

Purpose

Expression of full-length AsCas12f sgRNA in mammalian cells

Depositor

Weixin Tang

Article

Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

Insert

full-length AsCas12f sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMK287 (mAID-Hygro)

Plasmid

#72825

Purpose

C-terminal tagging with mAID using CRISPR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-Hygro

Use

CRISPR

Tags

mAID

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge