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TDP-REGv2(mScarlet-FLAG)#11 in pTwist-CMV backbone

Plasmid

#216155

Purpose

Expresses mScarlet in cells with TDP-43 loss-of-function. Very bright and decent dynamic range (>10-fold), slightly leaky. It uses a single intron. [Code 'B12']. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

Depositor

Pietro Fratta

Article

Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

Insert

mScarlet with cryptic splice site and C-terminal FLAG tag

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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+36GFP-LPETG

Plasmid

#71754

Purpose

+36GFP-LPETG for expression in E.Coli

Depositor

David Liu

Article

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

(GGS)9_(pos36GFP)_6XHis_LPETG

Use

Tags

6XHis and Sortase (LPETG)

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pTE102

Plasmid

#59397

Purpose

MethyloBrick expression vector, mxaF promoter; TcR

Depositor

Tobias Erb

Article

Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PmxaF

Available since

Dec. 30, 2014

Availability

Academic Institutions and Nonprofits only

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pUC19-eGFP-P2A-dTAG-Ing3

Plasmid

#170754

Purpose

Donor plasmid for eGFP-P2A-2xHA-dTAG knock-in into the N-terminus of the mouse Ing3 locus

Depositor

Kristian Helin

Article

Damhofer et al STAR Protoc. 2021 Jun 2;2(2):100575. doi

Insert

eGFP-P2A-2xHA-dTAG

Use

Mouse Targeting

Tags

eGFP-P2A-2xHA-dTAG

Expression

Mutation

Phenylalanine 36 to valine in FKBP12

Promoter

none

Available since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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TFORF3337

Plasmid

#144813

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

HDAC1 (HDAC1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-RACK1

Plasmid

#41088

Depositor

Anna Huttenlocher

Article

Cox et al Mol Biol Cell. 2003 Feb;14(2):658-69.

Insert

Receptor for activated C kinase (RACK1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pSWEET-bgaB

Plasmid

#210272

Purpose

Xylose-dependent expression system for tight and modulated expression of cloned genes in Bacillus subtilis.

Depositor

Eric Brown

Article

Bhavsar et al Appl Environ Microbiol. 2001 Jan;67(1):4

Insert

bgaB

Use

Tags

Expression

Bacterial

Mutation

Promoter

PxylA

Available since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pDS190

Plasmid

#34977

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

ePDZb

Use

Tags

Expression

Yeast

Mutation

Promoter

ADH

Available since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pKNOCK-Km

Plasmid

#46262

Purpose

Bacterial targeting

Depositor

Mikhail Alexeyev

Article

Alexeyev et al Biotechniques. 1999 May;26(5):824-6, 828

Type

Empty backbone

Use

Bacterial targeting

Tags

Expression

Mutation

Promoter

Available since

Aug. 14, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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IVT-MCP-Phi29

Plasmid

#207456

Purpose

in vitro transcription of mRNA

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

Insert

MCP-Phi29

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-ON-shCtrl (neo)

Plasmid

#192076

Purpose

Lentivirus for Dox-inducible expression of a non-targeting control shRNA

Depositor

Roland Friedel

Insert

non-targeting randomized sequence

Use

Lentiviral

Tags

Expression

Mutation

Promoter

H1 / Tet-responsive element

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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QUAS-jGCaMP7s-T2A-tdTomato

Plasmid

#200010

Purpose

QUAS reporter line expressing GCaMP7s calcium indicator and tdTomato, separated by T2A

Depositor

Carolyn McBride

Article

Zhao et al Nature. 2022 May;605(7911):706-712. doi:

Insert

3xP3-ECFP-jGCaMP7s-td-Tomato

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

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pmVenus-C2-MmMKL2

Plasmid

#67894

Purpose

Expresses a fusion of mVenus with MKL2 (MRTF-B) in mammalian cells

Depositor

Dorus Gadella

Article

van_Unen et al Scientific Reports 5, Article number: 14

Insert

mVenus-MKL2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pPT24-mtnluc

Plasmid

#184339

Purpose

Transformation of mitochondrial DNA in yeast

Depositor

Martin Ott

Article

Rzepka et al STAR Protoc. 2022 May 25;3(2):101359. do

Insert

nanoluciferase recoded for mitochondrial expression flanked by COX2 UTRs

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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Cas9 sgRNA vector

Plasmid

#68463

Purpose

Cas9 sgRNA vector for cloning

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-RAX-WT

Plasmid

#194572

Purpose

Mammalian Expression of mEGFP-RAX WT Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

RAX (RAX Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 EYFP H148Q/I152L

Plasmid

#25872

Depositor

Peter Haggie

Article

Galietta et al FEBS Lett. 2001 Jun 22. 499(3):220-4.

Insert

EYFP H148Q/I152L

Use

Tags

Expression

Mammalian

Mutation

EYFP from pEYFP (Clontech) with H148Q and I152L m…

Promoter

Available since

Aug. 16, 2010

Availability

Academic Institutions and Nonprofits only

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pLenti-MetGFP

Plasmid

#37560

Depositor

David Rimm

Insert

MET (MET Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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pBiFC-bJunVN173

Plasmid

#22012

Depositor

Chang-Deng Hu

Article

Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

Insert

bJunVN173 (Jun Rat, Aequorea Victoria)

Use

Tags

FLAG

Expression

Mammalian

Mutation

c-Jun(257-338)-Venus(1-172)

Promoter

Available since

Sept. 28, 2009

Availability

Academic Institutions and Nonprofits only

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Tubulin-hfYFP

Plasmid

#186515

Purpose

Localization of hyperfolder YFP fluorescent protein to tubulin

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Hyperfolder YFP

Use

Tags

Tubulin

Expression

Mammalian

Mutation

mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

Promoter

CMV

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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