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Plasmid#46499DepositorInsertSHP-2(NC) (PTPN11 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 1-222PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAG-AviMta3-3xFiBsd
Plasmid#140962Purposefull-length wt Avi-Mta3-3XFLAG-iBsd cDNA w/CAG promoter and Blasticidin resistance, cloned from mouse ES cellsDepositorInsertmetastasis associated 3 (Mta3 Synthetic, Mouse)
UseTags3xFLAG, Avi, and TEVExpressionMammalianMutationPromoterCAGAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_mGFP-FGFR3-G380R
Plasmid#191755PurposeExpression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-G380R mutation (associated with ACH; target mutation: c.1138G>A; silent mutation added on purpose: c.1131C>T)DepositorInsertmGFP-FGFR3IIIc
UseTagsmGFPExpressionMammalianMutationc.1138G>A (G380R) + c.1131C>T (silent mutat…PromoterAvailable sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEBTetBl-CLIP-ALMS1
Plasmid#136877PurposeMammalian expression of the centrosomal protein ALMS1 N-terminally fused to CLIP-tagDepositorInsertCLIP-ALMS1 (ALMS1 Synthetic, Human)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HsNot9_18-285_S
Plasmid#147480PurposeMammalian Expression of HsNot9_18-285DepositorInsertHsNot9_18-285 (CNOT9 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot9_18-285_S
Plasmid#147482PurposeMammalian Expression of HsNot9_18-285DepositorInsertHsNot9_18-285 (CNOT9 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-TEVsite-human Glomulin
Plasmid#52292Purposebacterial expression of human GlomulinDepositorInsertGlomulin (GLMN Human)
UseTagsGST and TEVExpressionBacterialMutationPromotertacAvailable sinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ABL1-1/1
Plasmid#91521PurposeProtein expression and purification of human SH3 domain construct ABL1-1/1DepositorInsertABL1-1/1 (ABL1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO-miR-124-sponge_rev(neg. CTRL)
Plasmid#117326PurposeDual luciferase assay negative control for miR-124 bindingDepositorInsertreverse miR-124 sponge
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterMurine PGK-1Available sinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST-DHFR F[1,2]-N (TRP1)
Plasmid#177797PurposeGateway destination vector to insert genes of interest having a N-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays.DepositorTypeEmpty backboneUseTagsDHFR F[1,2]ExpressionYeastMutationPromoterADH1Available sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AKAP79-AKAR4
Plasmid#181957PurposeGenetically encoded FRET-based PKA activity reporter fused to AKAP79.DepositorInsertAKAP79-AKAR4 (AKAP5 Human)
UseTagsAKAP79, Cerulean, HIS tag, and cpVenus(E172)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX Emt-SH2
Plasmid#46424DepositorInsertEmt (ITK Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 223-346PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-Cep76
Plasmid#136813PurposeMammalian expression of the centrosomal protein Cep76 N-terminally fused to SNAP-tagDepositorInsertSNAP-Cep76 (CEP76 Synthetic, Human)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
MBP-hnRNPA2_LC
Plasmid#98661PurposeExpresses MBP-tagged hnRNPA2 AA 190-341DepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTags6xHis-MBPExpressionBacterialMutationPromoterAvailable sinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO myc Separase Non-Cleavable
Plasmid#59826PurposeAllows the integration of myc Separase Non-Cleavable in the genome and Tet-inducible expression.DepositorInsertSeparase (ESPL1 Human)
UseTagsMycExpressionMammalianMutationNon-Cleavable (E1483R, R1486E, E1503R, R1506E, E1…Promoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX Lyn-SH2
Plasmid#46452DepositorInsertLyn (LYN Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 106-213PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
R711-X12-566: His6-MBP-tev-Hs.NF1iso2 (1203-1530)
Plasmid#159580PurposeE. coli protein expression of His6-MBP-tev-Hs.NF1iso2 (1203-1530)DepositorInsertHis6-MBP-tev-Hs.NF1iso2 (1203-1530) (NF1 Human)
UseTagsHis6-MBP-tevExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGS-CD64-2
Plasmid#109191PurposeEncodes full-length engineered CD64 with C-terminal Twin-Strep tag to be expressed in a baculovirus/insect cell expression systemDepositorInsertFull-length engineered CD64, derived from human CD64
UseTagsTwin-StrepExpressionInsectMutationengineered for efficient expression, see publicat…PromoterPolyhedrinAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
Flag Depdc5 pLJM1
Plasmid#46336DepositorInsertDepdc5 (DEPDC5 Human)
UseLentiviralTagsFlagExpressionMammalianMutationDiffers from Depdc5 isoform 1 by 10 amino acids …PromoterAvailable sinceJuly 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-hGTB
Plasmid#26196DepositorInsertTet transactivator, enhanced green fluorescent protein, TVA, rabies B19 glycoprotein
UseAAV and Cre/Lox; Adeno-associated virusTagsExpressionMutationF2A has 2A cleavage site deleted, producing htTA-…PromoterAvailable sinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only