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pEN-CAG-mRFP-PCNA pc2729

Plasmid

#166040

Purpose

Expresses mRFP tagged human PCNA in mammalian cells.

Depositor

Cristina Cardoso

Article

Heinz et al Nucleic Acids Res. 2018 Jul 6;46(12):611

Insert

Proliferating Cell Nuclear Antigen (PCNA Human)

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-FRISZ-GPI

Plasmid

#177264

Purpose

AAV transfer plasmid for cell-surface-localized FRISZ (via CD59 GPI) under hSyn promoter

Depositor

Huiwang Ai

Article

Wu et al Sci Adv. 2023 Mar;9(9):eadd2058. doi: 10

Insert

CD59 leader-FRISZ-GPI

Use

AAV

Tags

CD59 GPI and CD59 leader sequence

Expression

Mutation

Promoter

hSyn

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pS238D1::tse5-CT

Plasmid

#192958

Purpose

For expression of Tse5-CT (1169-1317) in P. putida for biological function studies

Depositor

David Albesa-Jové

Article

Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

Insert

tse5-CT

Use

Tags

Expression

Bacterial

Mutation

encodes for tse5 (1169-1317)

Promoter

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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H1.M10.luc

Plasmid

#193706

Purpose

Expressed a H1-core mutant by introducing multiple 7SK elements (PSE, spacer, and TATA box)

Depositor

Elena Herrera-Carrillo

Article

Gao et al J Biol Chem. 2020 Nov 5. pii: S0021-9258

Insert

H1-core promoter with multiple 7SK domains (PSE, spacer, and TATA box) + N41

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pLV sgRNA-Notch1 #2 GFP

Plasmid

#106951

Purpose

Lentivirus encoding sgRNA targeting murine Notch1. sgRNA is less optimal. Includes GFP marker.

Depositor

Albert Edge

Article

Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

Insert

gRNA targeting Notch1 (Notch1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pLV sgRNA-Notch1 #3 GFP

Plasmid

#106952

Purpose

Lentivirus encoding sgRNA targeting murine Notch1. sgRNA is less optimal. Includes GFP marker.

Depositor

Albert Edge

Article

Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

Insert

gRNA targeting Notch1 (Notch1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pKI-cjT-tdTomato-PGK-Neo

Plasmid

#186173

Purpose

Knock-in gene targeting in marmoset cells at TBXT locus

Depositor

Sho Yoshimatsu

Article

Yoshimatsu et al Biology (Basel). 2022 Jun 29;11(7):987.

Insert

tdTomato

Use

Marmoset targeting

Tags

Expression

Mutation

Promoter

Available since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pKI-cjOSR1-EGFP-PGK-Puro

Plasmid

#186170

Purpose

Knock-in gene targeting in marmoset cells at OSR1 locus

Depositor

Sho Yoshimatsu

Article

Yoshimatsu et al Biology (Basel). 2022 Jun 29;11(7):987.

Insert

EGFP

Use

Marmoset targeting

Tags

Expression

Mutation

Promoter

Available since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pKI-cjSF1-tdTomato-PGK-Neo

Plasmid

#186172

Purpose

Knock-in gene targeting in marmoset cells at SF1 locus

Depositor

Sho Yoshimatsu

Article

Yoshimatsu et al Biology (Basel). 2022 Jun 29;11(7):987.

Insert

tdTomato

Use

Marmoset targeting

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEY80

Plasmid

#191075

Purpose

flp-18(AVA = 4.2-1k) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

flp-18(AVA = 4.2-1k) (flp-18 Nematode)

Use

Tags

Expression

Worm

Mutation

None

Promoter

Available since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCALNL-tdTomato-2A-mSyp::EGFP

Plasmid

#191213

Purpose

Expresses tdTomato protein and mouse Synaptophysin protein fused with EGFP in Cre expressing mammalian cells.

Depositor

Theofanis Karayannis

Article

Gesuita et al STAR Protoc. 2022 Nov 11;3(4):101837. do

Insert

tdTomato-T2A-mSyp::EGFP (Syp Mouse)

Use

Pcagen

Tags

mSyp::EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.176

Plasmid

#184997

Purpose

Express -Eco1 RNF2 editing ncRNA and gRNA

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1: RNF2 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter

Use

Tags

SV40NLS

Expression

Mammalian

Mutation

RNF2 donor, a1/a2 length extended for 27 bp

Promoter

H1

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pEY73

Plasmid

#191070

Purpose

cho-1(3.7-1.7k) fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

cho-1(3.7-1.7k) (cho-1 Nematode)

Use

Tags

Expression

Worm

Mutation

None

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEY72

Plasmid

#191069

Purpose

cho-1(3.7k) fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

cho-1(3.7k) (cho-1 Nematode)

Use

Tags

Expression

Worm

Mutation

None

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pKI-cjSF1-hCD271-PGK-Neo

Plasmid

#186171

Purpose

Knock-in gene targeting in marmoset cells at SF1 locus

Depositor

Sho Yoshimatsu

Article

Yoshimatsu et al Biology (Basel). 2022 Jun 29;11(7):987.

Insert

hCD271

Use

Marmoset targeting

Tags

Expression

Mutation

human codon-optimized

Promoter

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pKI-cjFOXL2-EGFP-PGK-Puro

Plasmid

#186167

Purpose

Knock-in gene targeting in marmoset cells at FOXL2 locus

Depositor

Sho Yoshimatsu

Article

Yoshimatsu et al Biology (Basel). 2022 Jun 29;11(7):987.

Insert

EGFP

Use

Marmoset targeting

Tags

Expression

Mutation

Promoter

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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mU6-sgCebpa_v2-hu6-sgCebpb_v2

Plasmid

#177252

Purpose

Expresses Cebpa_v2 (mU6), Cebpb_v2(hU6) gRNAs and Cre-recombinase

Depositor

Monte Winslow

Article

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgCebpa_v2/sgCebpb_v2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

mU6/hU6

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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hu6-sgCebpa_v2 (opti)

Plasmid

#177246

Purpose

Same as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses Cebpa gRNA

Depositor

Monte Winslow

Article

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgCebpa_2nd

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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dora[A]_rescue_construct

Plasmid

#190639

Purpose

Puromycin-selectable expression of truncated Dora (CG34401) in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Dorado (CG34401 Fly)

Use

Tags

Expression

Insect

Mutation

Truncated to contain amino acids 1-945

Promoter

Actin-5c

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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HA-dora[A]_rescue_construct

Plasmid

#190640

Purpose

Puromycin-selectable expression of HA-tagged truncated Dora (CG34401) in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Dorado (CG34401 Fly)

Use

Tags

3xHA

Expression

Insect

Mutation

Truncated to contain amino acids 1-945

Promoter

Actin-5c

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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