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LoxP1-Mb2YFP-2A (SO244)

Plasmid

#99613

Purpose

To clone gene of interest downstream of LoxP1-Mb2YFP-2A cassette

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Mb2EYFP

Use

Tags

T2A

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A STING V155M 342STOP

Plasmid

#102594

Purpose

Lentivector to express STING V155M delta 342 and mTagBFP

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

V155M delta 342

Promoter

SFFV

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pnEA-vH_His-TEV-SEPT2

Plasmid

#174491

Purpose

bacterial expression of human SEPT2

Depositor

Manos Mavrakis

Article

Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

Insert

SEPTIN2 (SEPTIN2 Human)

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-Ezh2

Plasmid

#118587

Purpose

Expresses Ezh2 from the TRE3G promoter

Depositor

Stanley Qi

Article

Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

Insert

Ezh2 (Ezh2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC15A2

Plasmid

#131886

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC15A2 (SLC15A2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 9, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-pCMV-TM-Kv2.1-CaM-NES-TevN-AsLOV2-TEVseq-tTA

Plasmid

#171618

Purpose

AAV Soma-targeted Cal-Light with Kv2.1

Depositor

Hyungbae Kwon

Article

Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

Insert

TM-Kv2.1-CaM-NES-TevN-AsLOV2-TEVseq-tTA

Use

AAV and Synthetic Biology

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC25A48

Plasmid

#131995

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A48 (SLC25A48 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 22, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-F-ELL2

Plasmid

#49422

Purpose

Mammalian expression of flag-tagged human ELL2

Depositor

Joan Conaway, Ronald Conaway

Article

Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

Insert

ELL2 (ELL2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only
attB-mCh-Dnmt3b1-Poly(A)-PuroR

Plasmid

#65556

Purpose

Plasmid for Bxb1-mediated recombination of the mCh-Dnmt3b1 cDNA into a MIN-tagged locus using Puromycin selection

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

Dnmt3b (Dnmt3b Mouse)

Use

Mouse Targeting; Bxb1

Tags

mCherry

Expression

Mammalian

Mutation

isoform 1

Promoter

Available since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pETDuet1-WT-LanCL2-C-Ter-His

Plasmid

#154186

Purpose

To express LanCL2 in bacterial cells

Depositor

Wilfred van der Donk

Article

Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

Insert

LanCL2

Use

Tags

Hexahistidine

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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tdEos-Vimentin-7

Plasmid

#57688

Purpose

Localization: Intermediate Filaments, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

Vimentin (VIM Human)

Use

Tags

tdEos

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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tdEos-LaminB1-10

Plasmid

#57632

Purpose

Localization: Nuclear Envelope, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

LaminB1 (LMNB1 Human)

Use

Tags

tdEos

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 16, 2015

Availability

Industry, Academic Institutions, and Nonprofits
FLAG-KLF12/pcDNA3.1-Neo

Plasmid

#81069

Purpose

Expression of epitope-tagged human KLF12 in mammalian cells.

Depositor

Julian Downward

Article

Godin-Heymann et al Oncogene. 2016 Jun 23;35(25):3324-34. do

Insert

FLAG-KLF12 (KLF12 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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LoxP1-His-H2B-Cherry-2A (SO290)

Plasmid

#99616

Purpose

To clone gene of interest downstream of LoxP1-His-H2B-Cherry-2A cassette

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

6xHis-H2B-Cherry

Use

Tags

T2A

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCR8/GW/TOPO-ETV6

Plasmid

#98625

Purpose

Gateway entry TOPO TA cloning vector for ETV6

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

ETV6 (Etv6 Mouse)

Use

Topo ta cloning vector

Tags

Expression

Mutation

Promoter

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pRS0045

Plasmid

#131124

Purpose

Mammalian Target-ACE expression vector

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

TadA-linker(32aa)-TadA*(7.10)-linker(32aa)-nCas9-PmCDA1-UGI

Use

CRISPR

Tags

PmCDA1, TadA, and UGI

Expression

Mammalian

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V …

Promoter

CMV promoter

Available since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

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LoxP2-H2B-EGFP-V5-2A (SO107)

Plasmid

#99617

Purpose

To clone gene of interest downstream of LoxP2-H2B-EGFP-V5-2A

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

H2B-EGFP-V5

Use

Tags

T2A

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC16A7

Plasmid

#131947

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC16A7 (SLC16A7 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 17, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pMXS-IRES-Blast POLD1

Plasmid

#160805

Purpose

Express POLD1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

POLD1 (POLD1 Human)

Use

Retroviral

Tags

Expression

Mutation

Codon optimized C-terminal 230 amino acids

Promoter

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC10A4

Plasmid

#131943

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC10A4 (SLC10A4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 17, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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