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DYRK2 gRNA (BRDN0001149160)

Plasmid

#77537

Purpose

3rd generation lentiviral gRNA plasmid targeting human DYRK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DYRK2 (DYRK2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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DYRK2 gRNA (BRDN0001149060)

Plasmid

#77538

Purpose

3rd generation lentiviral gRNA plasmid targeting human DYRK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DYRK2 (DYRK2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
DYRK1A gRNA (BRDN0001149042)

Plasmid

#76756

Purpose

3rd generation lentiviral gRNA plasmid targeting human DYRK1A

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DYRK1A (DYRK1A Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
DYRK1B gRNA (BRDN0001149374)

Plasmid

#76624

Purpose

3rd generation lentiviral gRNA plasmid targeting human DYRK1B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DYRK1B (DYRK1B Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
DYRK3 gRNA (BRDN0001149001)

Plasmid

#75548

Purpose

3rd generation lentiviral gRNA plasmid targeting human DYRK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DYRK3 (DYRK3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
DYRK4 gRNA (BRDN0001149224)

Plasmid

#75521

Purpose

3rd generation lentiviral gRNA plasmid targeting human DYRK4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DYRK4 (DYRK4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
NEK1 gRNA (BRDN0001146251)

Plasmid

#76125

Purpose

3rd generation lentiviral gRNA plasmid targeting human NEK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NEK1 (NEK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
NEK1 gRNA (BRDN0001149235)

Plasmid

#76126

Purpose

3rd generation lentiviral gRNA plasmid targeting human NEK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NEK1 (NEK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
NEK1 gRNA (BRDN0001148922)

Plasmid

#76127

Purpose

3rd generation lentiviral gRNA plasmid targeting human NEK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NEK1 (NEK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLENTI EF1alpha FLAG/HA-LCOR

Plasmid

#114444

Purpose

Mammalian lentiviral expression vector containing N terminal FLAG/HA tagged human LCOR coding sequence

Depositor

Adrian Bracken

Article

Conway et al Mol Cell. 2018 May 3;70(3):408-421.e8. d

Insert

LCOR (LCOR Human)

Use

Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pRDA_103

Plasmid

#133460

Purpose

H1 promoter with two Tet operator (TetO) sites expresses customizable Spyo-guide; short EF1a promoter (EFS) expresses SaurCas9, 2A provides TetR, and 2A provides blasticidin resistance.

Depositor

John Doench

Article

DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

Insert

control guide

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
CBX1 A3.5 gRNA

Plasmid

#90566

Purpose

3rd generation lentiviral gRNA plasmid targeting human CBX1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CBX1 (Guide Designation A3.5)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
CBX1 A4.5 gRNA

Plasmid

#90567

Purpose

3rd generation lentiviral gRNA plasmid targeting human CBX1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CBX1 (Guide Designation A4.5)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
CBX5 A2.5 gRNA

Plasmid

#90571

Purpose

3rd generation lentiviral gRNA plasmid targeting human CBX5

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CBX5 (Guide Designation A2.5)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
CBX5 A1.5 gRNA

Plasmid

#90570

Purpose

3rd generation lentiviral gRNA plasmid targeting human CBX5

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CBX5 (Guide Designation A1.5)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
CBX3 A5.5 gRNA

Plasmid

#90568

Purpose

3rd generation lentiviral gRNA plasmid targeting human CBX3

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CBX3 (Guide Designation A5.5)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRRL_H1shLacZ_PGK_eGFP_Teto

Plasmid

#22038

Depositor

Didier Trono

Insert

shlacZ

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 14, 2009

Availability

Academic Institutions and Nonprofits only
pINDUCER20-Flag-NLS-hSPDEF delta1

Plasmid

#100874

Purpose

Lentiviral expression of human SPDEF truncation

Depositor

Noah Shroyer

Article

Lo et al Gastroenterology. 2017 Jul;153(1):205-21

Insert

SPDEF (SPDEF Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

truncated protein 1-248aa

Promoter

TRE2

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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PBK D1.3 gRNA

Plasmid

#90824

Purpose

3rd generation lentiviral gRNA plasmid targeting human PBK

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PBK (Guide Designation D1.3)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PRKAA1 gRNA (BRDN0001147273)

Plasmid

#76254

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKAA1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKAA1 (PRKAA1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge