-
Plasmid#122597Purposeclone M13 phage by SapI Golden GateDepositorInsertM13 bacteriophage genes
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0108
Plasmid#91001PurposeModule A, Promoter: EC1.2, Gene: AtCas9, Terminator: HSPDepositorInsertAtCas9
UseCRISPRTagsExpressionMutationPromoterEC1.2Available sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CMV-MSI1(FL)
Plasmid#60358Purposeexpress mouse MSI1 in mammalian cellsDepositorInsertmouse MSI1 (FL) (Msi1 Mouse)
UseTagsExpressionBacterialMutationPromoterCMVAvailable sinceNov. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE V5-epitope donor ORF0
Plasmid#200377PurposeTagging endogenously expressed proteins with V5 epitope, C-terminalDepositorInsertV5-epitope
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSimpleII-NLS-NmCas9-HA-NLS(s)
Plasmid#47867PurposeThis plasmid encode NmCas9 with two NLS and a HA tagDepositorInsertNmCas9
UseCRISPRTagsHA and NLSExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBTK1003 (pSL2)
Plasmid#190998PurposeExpresses red chromoprotein on a broad host range RSF1010 origin plasmidDepositorInsertred chromoprotein
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterCP25Available sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ135-tRNA2.0
Plasmid#158397PurposeGolden Gate entry vector to express the 5th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-3c-rhVTN-N
Plasmid#30225DepositorInsertrhVitronectin with N terminus cleavage (VTN Human)
UseTagsExpressionBacterialMutationdeleted amino acids: 20*-62PromoterAvailable sinceMay 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
Sa-SlugCas9
Plasmid#163797PurposeExpresses Sa-SlugCas9, and cloning backbone for sgRNADepositorTypeEmpty backboneUseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-SpyCatcher002 EQ
Plasmid#102830PurposeBacterial expression of unreactive version of SpyCatcher002DepositorInsertSpyCatcher002 EQ
UseTagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationE77Q mutation prevents isopeptide bond formationPromoterT7Available sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2092 - [1-2] ENTR - Fluor - mNeon(PATC)
Plasmid#159853PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - mNeon(PATC)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE V5-epitope donor ORF2
Plasmid#200379PurposeTagging endogenously expressed proteins with V5 epitope, C-terminalDepositorInsertV5-epitope
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-PGK-WP1-CRY2PHR-VP64
Plasmid#171758PurposeLentiviral vector for constitutive expression of fusion protein WP1-CRY2PHR-VP64DepositorInsertWP1-CRY2PHR-VP64
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mH6-Cas12c1
Plasmid#120872PurposeExpresses E. coli codon optimized Cas12c1 effector in the pET28a backbone.DepositorInsertCas12c1
UseCRISPRTagsmH6ExpressionBacterialMutationWTPromoterlacAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-PT2A-Tdtomato-iRFP670-GFP
Plasmid#111815PurposeTricistronic construct: Co-express three fluorescent proteins by P2A and then T2ADepositorInsertsTdtomato
iRFP670
GFP
UseCloning intermediateTagsExpressionMutationPromoterAvailable sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV- CAG-h2BmRuby2FLAG
Plasmid#116872PurposeNuclear localized mRuby2FLAGDepositorInsertH2BmRuby2FLAG
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmTFP1-ShadowG
Plasmid#99867PurposeMeasure FRET between mTFP1 and ShadowGDepositorInsertShadowG
UseTagsmTFP1ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBT276
Plasmid#122607Purposeexpress CDA1-BE4max in mammalian cellsDepositorInsertCDA1-BE4max
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS-EF1afull-H2B-mCherry-P2A-Fluc
Plasmid#207253PurposeLentiviral transfer genome encoding H2B-mCherry-P2A-Fluc driven by a full-length EF1a promoterDepositorInsertH2B-mCherry-P2A-Fluc
UseLentiviralTagsExpressionMammalianMutationPromoterEF1alpha_fullAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_FLT3
Plasmid#183293PurposeAll-in-One CRISPRko system with a guide RNA that targets FLT3 geneDepositorInsertFLT3
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only