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Plasmid#128324PurposeSP-dCas9 compatible gRNA to be used as a positive control for activation experiments (human cells)DepositorInsertgRNA against human ACTC1
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiGuideSLC35C1-neo
Plasmid#220868Purposeencodes CRISPR sgRNA targeting human SLC3C1DepositorInsertSLC35C1 (SLC35C1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-ePEG-LINE1-loxPsym
Plasmid#216866PurposeLentiviral guide RNA vector to insert the loxPsym sequence into LINE1 elementsDepositorInsertspacerLINE1_cr772scaffold_PBS-loxPsm-HA-tevopreQ
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGS96_EGFP(A206K)-I3-01 AAVS donor
Plasmid#211865PurposeI3-01 transgene integration at AAVS1 safe harbor locusDepositorInsertI3-01
UseCRISPRTagsExpressionMutationN/APromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pR6K-crRNA-CASTIF
Plasmid#199654PurposeR6K plasmid with a I-F CAST targeting lacZDepositorInsertCAST I-F systems
UseTagsExpressionBacterialMutationPromoterJ23119 promoterAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pR6K-crRNA-CASTIB
Plasmid#199656PurposeR6K plasmid with a I-B CAST targeting lacZDepositorInsertCAST I-B systems
UseTagsExpressionBacterialMutationPromoterJ23119 promoterAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
SGEP-sh-Hs-PHGDH-67
Plasmid#188671PurposeshRNADepositorInsertshPHGDH (PHGDH Human)
UseCRISPR and LentiviralTagsExpressionMutationWTPromoterAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
SGEP-sh-Hs-PHGDH-1968
Plasmid#188673PurposeshRNADepositorInsertshPHGDH (PHGDH Human)
UseCRISPR and LentiviralTagsExpressionMutationWTPromoterAvailable sinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
SGEP-sh-Hs-PHGDH-1957
Plasmid#188674PurposeshRNADepositorInsertshPHGDH (PHGDH Human)
UseCRISPR and LentiviralTagsExpressionMutationWTPromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
ipUSEPR-sg-Hs-PHGDH-2
Plasmid#188676PurposesgRNADepositorInsertsgPHGDH (PHGDH Human)
UseCRISPR and LentiviralTagsExpressionMutationWTPromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
SGEP-sh-Hs-PHGDH-1967
Plasmid#188672PurposeshRNADepositorInsertshPHGDH (PHGDH Human)
UseCRISPR and LentiviralTagsExpressionMutationWTPromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
ipUSEPR-sg-Hs-PHGDH-5
Plasmid#188679PurposesgRNADepositorInsertsgPHGDH (PHGDH Human)
UseCRISPR and LentiviralTagsExpressionMutationWTPromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
ipUSEPR-sg-Hs-PHGDH-6
Plasmid#188680PurposesgRNADepositorInsertsgPHGDH (PHGDH Human)
UseCRISPR and LentiviralTagsExpressionMutationWTPromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
ipUSEPR-sg-Hs-PHGDH-4
Plasmid#188678PurposesgRNADepositorInsertsgPHGDH (PHGDH Human)
UseCRISPR and LentiviralTagsExpressionMutationWTPromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
ipUSEPR-sg-Hs-PHGDH-3
Plasmid#188677PurposesgRNADepositorInsertsgPHGDH (PHGDH Human)
UseCRISPR and LentiviralTagsExpressionMutationWTPromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKSB- attP 3xP3-GFPsv40 GGGG AAGA
Plasmid#174534PurposeattP site and 3xP3-GFP fluorescence marker cassette for gene knock-in in mosquitoesDepositorInsertattP and 3xP3-GFP-SV40 BsaI cassette
UseTagsExpressionInsectMutationPromoter3xP3Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKSB- PUb-GFPsv40 GGGG AAGA
Plasmid#174536PurposePUb-GFP fluorescence marker cassette for gene knock-in in mosquitoesDepositorInsertPUb-GFP-SV40 BsaI cassette
UseTagsExpressionInsectMutationPromoterAedes aegypti PolyubiquitinAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKSB- PUb-GFPTub56D GGGG AAGA
Plasmid#174537PurposePUb-GFP fluorescence marker cassette for gene knock-in in mosquitoes, with DmTub56D terminatorDepositorInsertPUb-GFP-Tub56Dterminator BsaI cassette
UseTagsExpressionInsectMutationPromoterAedes aegypti PolyubiquitinAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
HCP2
Plasmid#166104PurposeThis plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombinationDepositorInsertMsn2-sgRNA#27 (MSN2 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
HCP3
Plasmid#166105PurposeThis plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombinationDepositorInsertMsn2-sgRNA#27 (MSN2 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only