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pCALNL-mutKir2.1
Plasmid#125579PurposeCre-dependent expression of a non-conducting mutant of Kir2.1 (FLAG-tagged) in mammalian cells.DepositorInsertKir2.1 (Kcnj2 Mouse)
UseCre/LoxTagsFLAGExpressionMammalianMutationA non-conducting mutant (GYG -> AAA).PromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLT 652
Plasmid#59997PurposeExpresses a hybrid dsRNA corresponding to the Caenorhabditis elegans dhc-3/him-8 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progenyDepositorUseTagsExpressionBacterialMutationpartial genomicPromoterAvailable sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAPM-D4 miR30-AGO2 ts1
Plasmid#115853PurposeAGO2 knockdownDepositorInsertAGO2 shRNA (AGO2 Human)
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX6p2rbs-GST-Ring1b(1-159)-Bmi1(1-109)
Plasmid#63139PurposeExpression of murine Ring 1b (1-159 aa) and Bmi1 (1-109 aa)DepositorUseTagsGSTExpressionBacterialMutationResidues 1-109 and Residues 1-159PromotertacAvailable sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
NC15 pGLUE CUL3
Plasmid#36970DepositorInsertCUL3 (CUL3 Human)
UseTagsCBP tag, HA, and streptagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEMS2144
Plasmid#104357PurposeAAV plasmid with smCBA promoter driving expression of emerald GFP (EmGFP) reporterDepositorInsertssAAV-smCBA-EmGFP
UseAAVTagsExpressionMutationPromotersmCBAAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX Hck-SH2
Plasmid#46445DepositorInsertHck (HCK Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 134-254PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ARHGAP32-1/1
Plasmid#91312PurposeProtein expression and purification of human SH3 domain construct ARHGAP32-1/1DepositorInsertARHGAP32-1/1 (ARHGAP32 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLJM1 FLCN (F118D) FLAG GFP
Plasmid#164979PurposeLentiviral vector for FLCN expression in mammalian cellsDepositorInsertFLCN (FLCN Human)
UseLentiviralTags1xFLAG-GFPExpressionMammalianMutationF118DPromoterUBCAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-AviMta2-3xFiBsd
Plasmid#140964Purposefull-length wt Avi-Mta2-3XFLAG-iBsd cDNA w/CAG promoter and Blasticidin resistance, cloned from mouse ES cellsDepositorInsertmetastasis-associated gene family, member 2 (Mta2 Mouse)
UseTags3xFLAG, Avi, and TEVExpressionMammalianMutationPromoterCAGAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
His-MBP human SPOP (aa 28-337)
Plasmid#52294Purposebacterial expression of human SPOP (aa 28-337) fused to His-MBPDepositorInsertSPOP (SPOP Human)
UseTagsHis-MBP and TEVExpressionBacterialMutationPromoterT7Available sinceApril 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
Nsp10-mCherry
Plasmid#165135Purposemammalian expression and localizationDepositorInsertNsp10 (ORF1ab codon optimized SARS-COV-2)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEBTetD-SNAP-Cep63
Plasmid#136812PurposeMammalian expression of the centrosomal protein Cep63 N-terminally fused to SNAP-tagDepositorInsertSNAP-Cep63 (CEP63 Synthetic, Human)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
Nsp10-EGFP
Plasmid#165115Purposemammalian expression and localizationDepositorInsertNsp10 (ORF1ab codon optimized SARS-COV-2)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro U6 sgRNA BfuAI stuffer A-tract
Plasmid#138525PurposeExpresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt control A-tract repeat RNA sequence.DepositorInsertControl non-PRC2 binding A-tract repeat RNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ASAP2-1/1
Plasmid#91504PurposeProtein expression and purification of human SH3 domain construct ASAP2-1/1DepositorInsertASAP2-1/1 (ASAP2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_MAP3K9-1/2
Plasmid#91313PurposeProtein expression and purification of human SH3 domain construct MAP3K9-1/2DepositorInsertMAP3K9-1/2 (MAP3K9 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ARHGAP12-1/1
Plasmid#91282PurposeProtein expression and purification of human SH3 domain construct ARHGAP12-1/1DepositorInsertARHGAP12-1/1 (ARHGAP12 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_AHI1-1/1
Plasmid#91269PurposeProtein expression and purification of human SH3 domain construct AHI1-1/1DepositorInsertAHI1-1/1 (AHI1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only
