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Showing: 11061 - 11080 of 20487 results

  1. pJEP322-pAAV-FullU6TO-SaCa9sgRNAi(emx1-sg1)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA

    Plasmid
    #113699
    Purpose
    Dox-inducible U6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1. Followed by an EFS driven GFP-KASH in a separate reading frame.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Inserts
    SaCas9 gRNA Cassete
    GFP
    Use
    AAV and CRISPR
    Tags
    KASH
    Expression
    Mutation
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113699-plasmid-map-sequence-id-229551

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  2. p426_Cas9_gRNA-R-ARS805a

    Plasmid
    #87408
    Purpose
    p426_Cas9_gRNA-ARS805a without ribozyme All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS805a sequence TTATTTGAATGATATTTAGT in yeast chromosome 8.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS805a
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87408-plasmid-map-sequence-id-193450

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  3. eSpCas9(1.1)_NoFlag_hROSA26_Left

    Plasmid
    #191442
    Purpose
    Expresses the hROSA26 left sgRNA in combination with FLAGless eSpCas9(1.1) to target the hRosa26 safe harbor locus
    Depositor
    Yannick Doyon
    Insert
    hROSA26 sgRNA
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    191442-plasmid-map-sequence-id-378118

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  4. pET-MBP-Geo_st

    Plasmid
    #87700
    Purpose
    Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP
    Depositor
    Jennifer Doudna
    Insert
    GeoCas9
    Use
    Tags
    10xHis-MBP-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87700-plasmid-map-sequence-id-174647

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  6. pSpCas9(BB)-2A-Neo

    Plasmid
    #127762
    Purpose
    Used for expression of sgRNA in mammalian cells
    Depositor
    Ken-Ichi Takemaru
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    127762-plasmid-map-sequence-id-247554

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  8. NM-01

    Plasmid
    #69799
    Purpose
    Bacterial expression of Cas9 with His, MBP, and Strep tags
    Depositor
    Gerard Cagney
    Insert
    Cas9
    Use
    Tags
    HIS, MBP, and StrepII
    Expression
    Bacterial
    Mutation
    Aspartate 10 to Alanine (D10A) and Histidine 840 …
    Promoter
    Available since
    Oct. 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  9. pDD400

    Plasmid
    #137024
    Purpose
    SapTrap mNG-GLO donor
    Depositor
    Daniel Dickinson, Bob Goldstein
    Insert
    C. elegans GLO-optimized mNeonGreen
    Use
    CRISPR; Saptrap donor for cloning repair templat…
    Tags
    C. elegans GLO-optimized mNeonGreen
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    137024-plasmid-map-sequence-id-274031

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  10. pMS051

    Plasmid
    #137025
    Purpose
    SapTrap mScarlet-I-GLO donor
    Depositor
    Daniel Dickinson, Bob Goldstein
    Insert
    C. elegans GLO-optimized mScarlet
    Use
    CRISPR; Saptrap donor for cloning repair templat…
    Tags
    C. elegans GLO-optimized mScarlet
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    137025-plasmid-map-sequence-id-274042

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  11. BII-gR-PnTW

    Plasmid
    #133356
    Purpose
    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications) coexpressed with tdTomato-NLS
    Depositor
    Blair Madison
    Insert
    tdTomato-NLS
    Use
    Tags
    HA-tagged tdTomato
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available since
    Jan. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    133356-plasmid-map-sequence-id-267393

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  12. pNSU299_SEO1

    Plasmid
    #166076
    Purpose
    Plasmid for constituive spCas9 and tet-inducible SEO1 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    SEO1 (SEO1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    166076-plasmid-map-sequence-id-329607

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  13. pnos-Fok1:dCas9-nos

    Plasmid
    #62210
    Purpose
    Expresses Fok1:dCas9 under control of nanos promoter and 3'UTR. For germ line restricted high specificity genome engineering in Drosophila melanogaster.
    Depositor
    Simon Bullock
    Insert
    Fok1-dCas9
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    nanos
    Available since
    March 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    62210-plasmid-map-sequence-id-101869

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  14. pLinker-BirA-3xHA-HXGPRT-loxP

    Plasmid
    #86668
    Purpose
    for generation of BirA tagged strains using CRISPR tagging technology
    Depositor
    David Sibley
    Insert
    Linker-BirA-3xHA-HXGPRT 3'UTR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    86668-plasmid-map-sequence-id-190141

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  15. ipUSEPR-sg-Hs-PHGDH-1

    Plasmid
    #188675
    Purpose
    sgRNA
    Depositor
    Lewis Cantley
    Insert
    sgPHGDH (PHGDH Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    188675-plasmid-map-sequence-id-375079

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  16. pDD346

    Plasmid
    #133311
    Purpose
    SapTrap mNG-C1 donor
    Depositor
    Daniel Dickinson
    Insert
    C. elegans codon-optimized mNeonGreen
    Use
    CRISPR; Saptrap donor for cloning repair templat…
    Tags
    C. elegans codon-optimized mNeonGreen
    Expression
    Worm
    Mutation
    Promoter
    None
    Available since
    Nov. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    133311-plasmid-map-sequence-id-263341

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  17. SGEP-sh-Hs-PHGDH-1956

    Plasmid
    #188670
    Purpose
    shRNA
    Depositor
    Lewis Cantley
    Insert
    shPHGDH (PHGDH Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    188670-plasmid-map-sequence-id-375063

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  18. SGEP-sh-Hs-PHGDH-1883

    Plasmid
    #188669
    Purpose
    shRNA
    Depositor
    Lewis Cantley
    Insert
    shPHGDH (PHGDH Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    188669-plasmid-map-sequence-id-375055

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  19. pLK89 - Edit-directing plasmid cloning insert

    Plasmid
    #98846
    Purpose
    Contains the insert used for step 2 of edit-directed plasmid cloning
    Depositor
    Leonid Kruglyak
    Insert
    KanR
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Changed sequence to remove a MluI cut site
    Promoter
    Available since
    July 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    98846-plasmid-map-sequence-id-189230

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  20. pDD373

    Plasmid
    #137021
    Purpose
    SapTrap GFP-GLO donor
    Depositor
    Daniel Dickinson, Bob Goldstein
    Insert
    C. elegans GLO-optimized GFP
    Use
    CRISPR; Saptrap donor for cloning repair templat…
    Tags
    C. elegans GLO-optimized GFP
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    137021-plasmid-map-sequence-id-274046

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Showing: 11061 - 11080 of 20487 results