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pCDNA3-SREBP-2-GFP (A1134-1135)

Plasmid

#204203

Purpose

Mammalian expression of SREBP-2

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SREBP-2 (SREBF2 Human)

Use

Tags

T7x3 and eGFP

Expression

Mammalian

Mutation

Gly to Ala 1134, 1135

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn SYT7alpha-HaloTag P2A PM-msGFP

Plasmid

#179717

Purpose

Lentiviral expression of mouse Syt7 with C terminal HaloTag and PM-targeted msGFP

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

SYT7 (Syt7 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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pTol2_elavl3(HuC)_NES-Caprola_on-mEGFP

Plasmid

#194697

Purpose

HuC driven expression of the consitutively active calcium recorder positive control Caprola_on fused to mEGFP for neuronal expression in zebrafish

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_on-mEGFP

Use

Zebrafish expression

Tags

mEGFP

Expression

Mutation

Promoter

elavl3(HuC)

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pTol2_elavl3(HuC)_NES-Caprola_off-mEGFP

Plasmid

#194698

Purpose

HuC driven expression of the inactive calcium recorder negative control Caprola_off fused to mEGFP for neuronal expression in zebrafish

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_off-mEGFP

Use

Zebrafish expression

Tags

mEGFP

Expression

Mutation

Promoter

elavl3(HuC)

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pJFRC7-20XUAS-IVS-NES_Caprola5-mEGFP

Plasmid

#205704

Purpose

Gal4 driven expression of EGFP-Caprola5 for targeted expression in Drosophila flies

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola5-EGFP

Use

Tags

Expression

Insect

Mutation

Promoter

20 Gal4

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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mpeg:lamp1b-mCherry-stop; cmlc2:GFP

Plasmid

#213739

Purpose

Expresses Lamp1b-mCherry in the macrophage lineage of zebrafish with a green heart transgenesis marker

Depositor

William Talbot

Article

Iyer et al Sci Adv. 2022 Sep 2;8(35):eabp8321. doi:

Insert

lamp1b-mCherry

Use

Zebrafish

Tags

Expression

Mutation

Promoter

mpeg1.1

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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mpeg:lamp2-mCherry-stop; cmlc2:GFP

Plasmid

#213740

Purpose

Expresses Lamp2-mCherry in the macrophage lineage of zebrafish with a green heart transgenesis marker

Depositor

William Talbot

Article

Iyer et al Sci Adv. 2022 Sep 2;8(35):eabp8321. doi:

Insert

lamp2-mCherry

Use

Zebrafish

Tags

Expression

Mutation

Promoter

mpeg1.1

Available since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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mpeg:Rab7-stop-polyA; cmlc2:GFP

Plasmid

#213738

Purpose

Expresses mCherry-Rab7 in the macrophage lineage of zebrafish with a green heart transgenesis marker

Depositor

William Talbot

Article

Iyer et al Sci Adv. 2022 Sep 2;8(35):eabp8321. doi:

Insert

mCherry-Rab7

Use

Zebrafish

Tags

Expression

Mutation

Promoter

mpeg1.1

Available since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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mpeg:Rab5c-stop-polyA; cmlc2:GFP

Plasmid

#213737

Purpose

Expresses mCherry-Rab5c in the macrophage lineage of zebrafish with a green heart transgenesis marker

Depositor

William Talbot

Article

Iyer et al Sci Adv. 2022 Sep 2;8(35):eabp8321. doi:

Insert

mCherry-Rab5c

Use

Zebrafish

Tags

mCherry

Expression

Mutation

Promoter

mpeg1.1

Available since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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plx208-CMV-SspB-EGFP-VP16

Plasmid

#213532

Purpose

Expresses one of the split transcription factor component in mammalian cells

Depositor

Wenjing Wang

Article

Shen et al Nat Methods. 2023 Jan;20(1):112-122. doi

Insert

SspB-EGFP-VP16

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pHyg-CUbo(K48R/K63R)-yeGFP

Plasmid

#212769

Purpose

C-terminal PCR-based tagging with CUbo(K48R/K63R)-yeGFP in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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px552-U6-gRNA1-U6-gRNA2-CMV-eGFP

Plasmid

#211760

Purpose

Paired gRNAs (sgRNA1 and 2) targeting Exon 1 of VEGFA gene conserved across mouse, rhesus macaque, and human.

Depositor

Glenn Yiu

Article

Chung et al Mol Ther Nucleic Acids. 2022 Apr 27;28:6

Insert

gRNA1 and gRNA2 targeting VEGF-A (VEGFA Human, Mouse, M. mulatta (rhesus macaque))

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 6, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG GFP minimal CMV pCDNA5

Plasmid

#212086

Purpose

LR vector for integration of GFP into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pCaUra3-CUbo(K48R/K63R)-yeGFP

Plasmid

#212774

Purpose

C-terminal PCR-based tagging with CUbo(K48R/K63R)-yeGFP in budding yeast with CaUra3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pHyg-CUbo(K48R)-yeGFP-His6

Plasmid

#212783

Purpose

C-terminal PCR-based tagging with CUbo(K48R)-yeGFP-His6 in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHyg-CUbo(K63R)-yeGFP-His6

Plasmid

#212784

Purpose

C-terminal PCR-based tagging with CUbo(K63R)-yeGFP-His6 in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
YIp128-CUP1-His7-NUbo-GFP

Plasmid

#212791

Purpose

Copper-inducible expression of the artificial test substrate His7-NUbo-GFP in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

His7-NUbo-GFP

Use

Integrative vector

Tags

Expression

Yeast

Mutation

Promoter

CUP1

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-CUP1-His6-CUbo-GFP

Plasmid

#212792

Purpose

Copper-inducible expression of the artificial test substrate His6-CUbo-GFP in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

His6-CUbo-GFP

Use

Integrative vector

Tags

Expression

Yeast

Mutation

Promoter

CUP1

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-CUP1-CUbo-AID*-GFP

Plasmid

#212796

Purpose

Copper-inducible expression of the artificial test substrate His6-CUbo-AID*-GFP in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

CUbo-AID*-GFP

Use

Integrative vector

Tags

Expression

Yeast

Mutation

Promoter

CUP1

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pRN3P-NLS-EGFP-Btgh-3NLS

Plasmid

#194469

Purpose

Encodes for bacterial O-GlcNAcase BtGH84 fused to enhanced GFP and NLSs for effective nuclear localization, in backbone for T3 In Vitro Transcription.

Depositor

Mathieu Boulard

Article

Formichetti et al Dev Cell 10 Jan 2024

Insert

BtGH84

Use

For in vitro transcription from t3 promoter after…

Tags

SV40 Nuclear Localization Signal, SV40 Nuclear Lo…

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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