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CSNK2A1 gRNA (BRDN0001146321)

Plasmid

#76675

Purpose

3rd generation lentiviral gRNA plasmid targeting human CSNK2A1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CSNK2A1 (CSNK2A1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-sgSIK3-A

Plasmid

#138682

Purpose

Expresses a mouse SIK3-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK3 mouse (Sik3 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-sgSIK3-C

Plasmid

#138683

Purpose

Expresses a mouse SIK3-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK3 mouse (Sik3 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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FBXO5 D3.4 gRNA

Plasmid

#90686

Purpose

3rd generation lentiviral gRNA plasmid targeting human FBXO5

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

FBXO5 (Guide Designation D3.4)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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MAPRE1 F9.3 gRNA

Plasmid

#90746

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPRE1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

MAPRE1 (Guide Designation F9.3)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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ZW10 H10.3 gRNA

Plasmid

#90943

Purpose

3rd generation lentiviral gRNA plasmid targeting human ZW10

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

ZW10 (Guide Designation H10.3)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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SKA1 H3.1 gRNA

Plasmid

#90888

Purpose

3rd generation lentiviral gRNA plasmid targeting human SKA1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SKA1 (Guide Designation H3.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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pCW-eGFP-SHLD2-m1

Plasmid

#114120

Purpose

Lentiviral vector for inducible expression of N-terminally tagged eGFP-SHLD2, m1 mutant

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD2 (m1 mutant) (SHLD2 Human)

Use

Lentiviral; Doxycycline inducible

Tags

eGFP

Expression

Mammalian

Mutation

W489A, F494A, W495A mutations introduced

Promoter

TRE promoter, Tet ON

Available since

Aug. 28, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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PKD2 gRNA (BRDN0001148691)

Plasmid

#76319

Purpose

3rd generation lentiviral gRNA plasmid targeting human PKD2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PKD2 (PKD2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCWXPGR-pTF-Epo

Plasmid

#114290

Purpose

TET-inducible expression of Erythropoietin

Depositor

Patrick Salmon

Insert

Erythropoietin (Epo Rat)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pLenCMV-Mars1CyeGFP

Plasmid

#75224

Purpose

Lentiviral transfer vector encoding Mars1Cy-eGFP fusion for intracellular expression

Depositor

Alan Waggoner

Article

Zhang et al J Clin Invest. 2015 Oct 1;125(10):3915-2

Insert

Mars1Cy-eGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 31, 2016

Availability

Academic Institutions and Nonprofits only

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pAWp7-7

Plasmid

#72739

Purpose

pFUGW-UBCp-RFP-[miR-124 sensor]-CMVp-GFP

Depositor

Timothy Lu

Article

Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

Insert

UBCp-RFP-[miR-124 sensor]-CMVp-GFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pAWp7-12

Plasmid

#72744

Purpose

pFUGW-UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-124]

Depositor

Timothy Lu

Article

Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

Insert

UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-124]

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAWp7-10

Plasmid

#72742

Purpose

pFUGW-UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-124]

Depositor

Timothy Lu

Article

Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

Insert

UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-124]

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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ATMIN gRNA (BRDN0001147857)

Plasmid

#75860

Purpose

3rd generation lentiviral gRNA plasmid targeting human ATMIN

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ATMIN (ATMIN Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pRRLNeo-pEF1a-p53ashL344P-mKate2-splitmVenusN

Plasmid

#69586

Purpose

Expresses mutated p53-L344P tagged with mKate2 and split N-term mVenus

Depositor

Galit Lahav

Article

Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

Insert

p53-L344P (TP53 Human)

Use

Lentiviral

Tags

mKate2 and split mVenus - N term

Expression

Mammalian

Mutation

p53 L344P mutation that prevents dimerization and…

Promoter

EF1alpha

Available since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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pRRL_H1shmp53_PGK_eGFP_Teto

Plasmid

#22039

Depositor

Didier Trono

Insert

shmp53

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
pLVTHM-shREV3-4

Plasmid

#38033

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Knobel et al Neoplasia. 2011 Oct;13(10):961-70.

Insert

shREV3-4 (Rev3l, Rev3l, REV3L human, Rat, Human, Mouse)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pLenti4/V5-DEST-P85(N564K)

Plasmid

#40226

Depositor

Lynda Chin

Insert

P85(N564K) (PIK3R1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

changed Asparagine 564 to Lysine

Promoter

CMV

Available since

Oct. 11, 2012

Availability

Academic Institutions and Nonprofits only
TET-pLKO.1 PURO shGDF11 #1

Plasmid

#83083

Purpose

Lentiviral shRNA vector for inducible knockdown of human GDF11 (cross reacts with mouse Gdf11)

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shGDF11 (GDF11, Gdf11 Human, Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only