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Showing: 11041 - 11060 of 52004 results

  1. bU6-sgLkb1_2nd-mU6-sgNeo1-hU6-sgNT

    Plasmid
    #177228
    Purpose
    Expresses Lkb1(bU6), neomycin (mU6) and non-targeting(hU6) gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgLkb1_2nd/sgNeo1/sgNT1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    bU6/mU6/hU6
    Available since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    177228-plasmid-map-sequence-id-351921

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  2. pSBbi-RB-PE2

    Plasmid
    #173902
    Purpose
    SB-transposon with constitutive expression of SpCas9 PE2
    Depositor
    Ronald Cohn
    Insert
    PE2
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    173902-plasmid-map-sequence-id-346342

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  3. pB2H12

    Plasmid
    #163837
    Purpose
    Two-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and PTPN12
    Depositor
    Jerome Fox
    Insert
    SpecR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    PTPN12 E57D
    Promoter
    Pro1;ProD;pLacZ-Opt
    Available since
    July 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    163837-plasmid-map-sequence-id-337607

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  4. pB2H6

    Plasmid
    #163835
    Purpose
    Two-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and SHP1
    Depositor
    Jerome Fox
    Insert
    SpecR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pro1;ProD;pLacZ-Opt
    Available since
    July 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    163835-plasmid-map-sequence-id-337603

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  5. pmCellFree_KA1_SARS-COV-2_ORF9c

    Plasmid
    #169397
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF9c with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _ORF9c
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    July 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    169397-plasmid-map-sequence-id-336871

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  6. pmCellFree_KA1_SARS-COV-2_NSP14

    Plasmid
    #169387
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP14 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _NSP14
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    169387-plasmid-map-sequence-id-336501

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  7. pmCellFree_KA1_SARS-COV-2_NSP7

    Plasmid
    #169381
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP7 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _NSP7
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Promoter
    T7- CMV/CAG
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    169381-plasmid-map-sequence-id-336497

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  8. pNSU299_MET16

    Plasmid
    #166091
    Purpose
    Plasmid for constituive spCas9 and tet-inducible MET16 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    MET16 (MET16 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    May 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166091-plasmid-map-sequence-id-329609

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  9. pMCSG53_Nsp3_Y_3

    Plasmid
    #167263
    Purpose
    Bacterial expression of Nsp3 Y domain (residues 1844-1945)
    Depositor
    Alexei Savchenko
    Insert
    Nsp3 (residues 1844-1945) (ORF1ab SARS-CoV-2)
    Use
    Tags
    His6-TEV site
    Expression
    Bacterial
    Mutation
    none
    Promoter
    Available since
    May 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    167263-plasmid-map-sequence-id-331999

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  10. pMCSG53_Nsp3_Y_2

    Plasmid
    #167262
    Purpose
    Bacterial expression of Nsp3 Y domain (residues 1619-1945)
    Depositor
    Alexei Savchenko
    Insert
    Nsp3 (residues 1619-1945) (ORF1ab SARS-CoV-2)
    Use
    Tags
    His6-TEV site
    Expression
    Bacterial
    Mutation
    none
    Promoter
    Available since
    May 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    167262-plasmid-map-sequence-id-331997

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  11. pMCSG53_Nsp3_Ubl1

    Plasmid
    #167257
    Purpose
    Bacterial expression of Nsp3 ubiquitin-like domain (1-111)
    Depositor
    Alexei Savchenko
    Insert
    Nsp3 (residues 1-111) (ORF1ab SARS-CoV-2)
    Use
    Tags
    His6-TEV site
    Expression
    Bacterial
    Mutation
    none
    Promoter
    Available since
    April 27, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    167257-plasmid-map-sequence-id-331343

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  12. pMCSG53_Nsp3_Y_1

    Plasmid
    #167261
    Purpose
    Bacterial expression of Nsp3 Y domain (residues 1584-1945)
    Depositor
    Alexei Savchenko
    Insert
    Nsp3 (residues 1584-1945) (ORF1ab SARS-CoV-2)
    Use
    Tags
    His6-TEV site
    Expression
    Bacterial
    Mutation
    none
    Promoter
    Available since
    April 27, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    167261-plasmid-map-sequence-id-331341

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  13. pMCSG53_Nsp3_NAB_2

    Plasmid
    #167260
    Purpose
    Bacterial expression of Nsp3 Nucleic acid binding domain (residues 1089-1203)
    Depositor
    Alexei Savchenko
    Insert
    Nsp3 (residues 1089-1203) (ORF1ab SARS-CoV-2)
    Use
    Tags
    His6-TEV site
    Expression
    Bacterial
    Mutation
    none
    Promoter
    Available since
    April 27, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    167260-plasmid-map-sequence-id-331337

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  14. pMCSG53_Nsp3_NAB_1

    Plasmid
    #167259
    Purpose
    Bacterial expression of Nsp3 Nucleic acid binding domain (residues 1050-1216)
    Depositor
    Alexei Savchenko
    Insert
    Nsp3 (residues 1050-1216) (ORF1ab SARS-CoV-2)
    Use
    Tags
    His6-TEV site
    Expression
    Bacterial
    Mutation
    none
    Promoter
    Available since
    April 27, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    167259-plasmid-map-sequence-id-331336

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  15. HCP1

    Plasmid
    #166103
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination.
    Depositor
    Matthias Heinemann
    Insert
    Msn2-sgRNA#27 (MSN2 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166103-plasmid-map-sequence-id-330378

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  16. pNSU299_CPR1_1

    Plasmid
    #166074
    Purpose
    Plasmid for constituive spCas9 and tet-inducible CPR1 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    CPR1 (CPR1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166074-plasmid-map-sequence-id-330357

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  17. pNSU299_RRT8

    Plasmid
    #166080
    Purpose
    Plasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of RRT8 for double stranded break formation in yeast.
    Depositor
    Rebecca Burgess
    Insert
    Promoter of RRT8 (RRT8 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166080-plasmid-map-sequence-id-330356

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  18. pNSU299_CPR1_2

    Plasmid
    #166071
    Purpose
    Plasmid for constituive spCas9 expression and tet-inducible expression of an sgRNA targeting an intergenic site near CPR1 for double stranded break formation in yeast.
    Depositor
    Rebecca Burgess
    Insert
    Intergenic region near CPR1
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166071-plasmid-map-sequence-id-330355

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  19. pNSU299_GAL7

    Plasmid
    #166089
    Purpose
    Plasmid for constituive spCas9 and tet-inducible GAL7 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    GAL7 (GAL7 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166089-plasmid-map-sequence-id-330353

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  20. pNSU299_GLK1

    Plasmid
    #166090
    Purpose
    Plasmid for constituive spCas9 and tet-inducible GLK1 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    GLK1 (GLK1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    166090-plasmid-map-sequence-id-329612

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Showing: 11041 - 11060 of 52004 results