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Cav2.2 HA D122A pcDNA3

Plasmid

#206108

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1

Depositor

Annette Dolphin

Article

Dahimene et al Cell Rep. 2018 Nov 6;25(6):1610-1621.e5.

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

D122A

Promoter

CMV

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pLJM1 FLCN (R164A) FLAG GFP

Plasmid

#164980

Purpose

Lentiviral vector for FLCN expression in mammalian cells

Depositor

Roberto Zoncu

Article

Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

Insert

FLCN (FLCN Human)

Use

Lentiviral

Tags

1xFLAG-GFP

Expression

Mammalian

Mutation

R164A

Promoter

UBC

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-Nsp12

Plasmid

#165117

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp12 (ORF1ab codon optimized SARS-COV-2)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pSLQ2842 pPB: CAG-GID1-VPR-IRES-Zeo-WPRE PGK-GAI-tagBFP-SadCas9

Plasmid

#84244

Purpose

Expresses GA-inducible VPR-Sa dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

GAI-tagBFP-Sa dCas9
GID1-VPR

Use

CRISPR and Synthetic Biology; Piggybac

Tags

GAI-tagBFP-Nucleoplasmin NLS, GID1, IRES-Zeocin, …

Expression

Mammalian

Mutation

D10A, N580A and Synonymous mutation in PYL1 to re…

Promoter

CAG and PGK

Available since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-tdTomato-f

Plasmid

#127092

Purpose

An AAV genome with tet-inducible, Cre-dependent expression of farnesylated (f) tdTomato

Depositor

Viviana Gradinaru

Article

Rajendran et al Nat Commun. 2019 Apr 26;10(1):1944. doi:

Insert

tdTomato-f

Use

AAV

Tags

farnesylation signal from c-Ha-Ras

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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EGFP-Nsp8

Plasmid

#165114

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp8 (ORF1ab codon optimized SARS-COV-2)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCALNL-mutKir2.1

Plasmid

#125579

Purpose

Cre-dependent expression of a non-conducting mutant of Kir2.1 (FLAG-tagged) in mammalian cells.

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

Kir2.1 (Kcnj2 Mouse)

Use

Cre/Lox

Tags

FLAG

Expression

Mammalian

Mutation

A non-conducting mutant (GYG -> AAA).

Promoter

Available since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLT 652

Plasmid

#59997

Purpose

Expresses a hybrid dsRNA corresponding to the Caenorhabditis elegans dhc-3/him-8 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progeny

Depositor

Lisa Timmons

Article

Timmons et al FEBS Lett. 2014 Jul 24. pii: S0014-5793(

Inserts

dhc-3 (dhc-3 Nematode)
him-8 (him-8 Nematode)

Use

Tags

Expression

Bacterial

Mutation

partial genomic

Promoter

Available since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-AGO2 ts1

Plasmid

#115853

Purpose

AGO2 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

AGO2 shRNA (AGO2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX6p2rbs-GST-Ring1b(1-159)-Bmi1(1-109)

Plasmid

#63139

Purpose

Expression of murine Ring 1b (1-159 aa) and Bmi1 (1-109 aa)

Depositor

Titia Sixma

Article

Buchwald et al EMBO J. 2006 Jun 7;25(11):2465-74. Epub

Inserts

Ring1b (Ring1 Mouse)
Bmi1 (Bmi1 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Residues 1-109 and Residues 1-159

Promoter

tac

Available since

March 16, 2015

Availability

Academic Institutions and Nonprofits only
NC15 pGLUE CUL3

Plasmid

#36970

Depositor

Randall Moon

Article

Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

Insert

CUL3 (CUL3 Human)

Use

Tags

CBP tag, HA, and streptag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 10, 2012

Availability

Academic Institutions and Nonprofits only
pEMS2144

Plasmid

#104357

Purpose

AAV plasmid with smCBA promoter driving expression of emerald GFP (EmGFP) reporter

Depositor

Elizabeth Simpson

Article

de Leeuw et al Mol Brain. 2016 May 10;9(1):52. doi: 10.

Insert

ssAAV-smCBA-EmGFP

Use

AAV

Tags

Expression

Mutation

Promoter

smCBA

Available since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pHH0103_ARHGAP32-1/1

Plasmid

#91312

Purpose

Protein expression and purification of human SH3 domain construct ARHGAP32-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ARHGAP32-1/1 (ARHGAP32 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX Hck-SH2

Plasmid

#46445

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Hck (HCK Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 134-254

Promoter

Tac

Available since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pLJM1 FLCN (F118D) FLAG GFP

Plasmid

#164979

Purpose

Lentiviral vector for FLCN expression in mammalian cells

Depositor

Roberto Zoncu

Article

Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

Insert

FLCN (FLCN Human)

Use

Lentiviral

Tags

1xFLAG-GFP

Expression

Mammalian

Mutation

F118D

Promoter

UBC

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-AviMta2-3xFiBsd

Plasmid

#140964

Purpose

full-length wt Avi-Mta2-3XFLAG-iBsd cDNA w/CAG promoter and Blasticidin resistance, cloned from mouse ES cells

Depositor

Brian Hendrich

Article

Burgold et al EMBO J. 2019 Jun 17;38(12). pii: embj.20

Insert

metastasis-associated gene family, member 2 (Mta2 Mouse)

Use

Tags

3xFLAG, Avi, and TEV

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pEBTetD-SNAP-Cep63

Plasmid

#136812

Purpose

Mammalian expression of the centrosomal protein Cep63 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Cep63 (CEP63 Synthetic, Human)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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Nsp10-mCherry

Plasmid

#165135

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp10 (ORF1ab codon optimized SARS-COV-2)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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His-MBP human SPOP (aa 28-337)

Plasmid

#52294

Purpose

bacterial expression of human SPOP (aa 28-337) fused to His-MBP

Depositor

Brenda Schulman

Article

Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1

Insert

SPOP (SPOP Human)

Use

Tags

His-MBP and TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 11, 2014

Availability

Academic Institutions and Nonprofits only
pHH0103_ASAP2-1/1

Plasmid

#91504

Purpose

Protein expression and purification of human SH3 domain construct ASAP2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ASAP2-1/1 (ASAP2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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