Skip to main content
Addgene
Showing: 11021 - 11040 of 13552 results

  6. 5JJW (USP15_SART3)

    Plasmid
    #101880
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    USP15_SART3 (USP15, SART3 Human)
    Use
    Tags
    6xHis, TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    101880-plasmid-map-sequence-id-194708

    Enlarge

  10. pJK605

    Plasmid
    #72530
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS); glutaminase subunit (AaPurQ); large subuint (AaPurL)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Use
    Tags
    Expression
    Bacterial
    Mutation
    synthetic gene
    Promoter
    T7 and n/a
    Available since
    April 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    72530-plasmid-map-sequence-id-141993

    Enlarge

  11. pJK623

    Plasmid
    #72540
    Purpose
    Produces Acetobacter aceti 1023 purSyQL associated hypothetical protein; formylglycinamidine ribonucleotide synthetase glutaminase subunit; large subunit (AaOrfY, AaPurQ, AaPurL)
    Depositor
    T. J. Kappock
    Inserts
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Use
    Tags
    Expression
    Bacterial
    Mutation
    synthetic gene
    Promoter
    T7 and n/a
    Available since
    March 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    72540-plasmid-map-sequence-id-132632

    Enlarge

  12. pJK289

    Plasmid
    #71553
    Purpose
    Production of Acetobacter aceti 1023 alanine racemase (alr) with Lys39>Ala mutant (AaAlr-K39A)
    Depositor
    T. J. Kappock
    Article
    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48
    Insert
    alanine racemase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes lysine-39 to alanine (please see deposito…
    Promoter
    Available since
    March 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    71553-plasmid-map-sequence-id-139945

    Enlarge

  13. pJK501

    Plasmid
    #72246
    Purpose
    Produces E. coli N5-carboxyaminoimidazole ribonucleotide mutase, Trp151>Phe mutant (EcPurE1-W151F), and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)
    Depositor
    T. J. Kappock
    Inserts
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Gln206 to Arg and changes tryptophan-151 to pheny…
    Promoter
    T7 and endogenous
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72246-plasmid-map-sequence-id-139355

    Enlarge

  15. pJK304

    Plasmid
    #72225
    Purpose
    Production of Acetobacter aceti 1023 alanine racemase (alr) with Ala368>Asp mutant (H6AaAlr-A368D)
    Depositor
    T. J. Kappock
    Article
    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48
    Insert
    alanine racemase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes alanine-368 to aspartate
    Promoter
    T7
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    72225-plasmid-map-sequence-id-134624

    Enlarge

  16. pJK303

    Plasmid
    #72224
    Purpose
    Production of Acetobacter aceti 1023 alanine racemase (alr) with Ala367>Asp mutant (H6AaAlr-A367D)
    Depositor
    T. J. Kappock
    Article
    Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48
    Insert
    alanine racemase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes alanine-367 to aspartate
    Promoter
    T7
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    72224-plasmid-map-sequence-id-134623

    Enlarge

  17. pJK579

    Plasmid
    #72426
    Purpose
    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag (AarCH6), additional unique internal restriction enzyme sites
    Depositor
    T. J. Kappock
    Insert
    succinyl-CoA:acetate CoA-transferase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    4 silent mutations introduce unique internal SpeI…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72426-plasmid-map-sequence-id-133996

    Enlarge

  18. pJK522

    Plasmid
    #72510
    Purpose
    Encodes Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit with N-terminal His6 tag (H6AaPurS); hypothetical protein with N-terminal His6 tag (H6AaOrfY), synthetic
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    synthetic gene
    Promoter
    T7 and n/a
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72510-plasmid-map-sequence-id-132535

    Enlarge

  19. pJK600

    Plasmid
    #71605
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Asn/Tyr154>Phe/Trp165>Phe mutant (AaPurE1fwf-H59N,Y154F)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes tryptophan-34 to phenylalanine, histidine…
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71605-plasmid-map-sequence-id-131682

    Enlarge

  20. pTmaM4

    Plasmid
    #67714
    Purpose
    Expression vector hexahistidine tagged variant of the Thermotoga maritima M-domain protein
    Depositor
    Robert Batey, Jeffrey Kieft
    Article
    Kieft et al RNA. 2004 Jun;10(6):988-95.
    Insert
    Thermotoga marítima Ffh-M domain
    Use
    Tags
    hexahistidine tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 RNA polymerase
    Available since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    67714-plasmid-map-sequence-id-126589

    Enlarge

Showing: 11021 - 11040 of 13552 results