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Plasmid#172470PurposeExpresses SpCas9, GFP, and sgRNA targeting Ago1DepositorInsertsgAgo1
UseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458_sgRNA_Ago1_6
Plasmid#172471PurposeExpresses SpCas9, GFP, and sgRNA targeting Ago1.DepositorInsertsgAgo1
UseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
PROM5_MprbcL_short_chlo
Plasmid#165161PurposeL0 part - promoterDepositorInsertMarchantia polymorpha rbcL promoter region
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
5UTR_Mprbcl_chlo
Plasmid#163934PurposeL0 part - 5'UTRDepositorInsertMarchantia polymorpha rbcL 5'UTR
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
PROM_Ntpsba_chlo
Plasmid#163950PurposeL0 part - promoterDepositorInsertNicotiana tabacum psbA promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
5UTR_Mppetb_short_chlo
Plasmid#163933PurposeL0 part - 5'UTRDepositorInsertMarchantia polymorpha petB 5'UTR
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCS2+-wbp2nlY282F
Plasmid#172468Purposeexpresses protein with one aa substitution to affect potential phosphorylation in WW-interaction domainDepositorInsertwbp2nlY282F
UseXenopus expressionTagsnoneExpressionMutationchanged Tyrosine at aa282 to PhenylalaninePromoterSP6Available sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-wbp2nlF127P
Plasmid#174507Purposeexpresses protein with one aa substitution to disrupt the alpha-helix near the PH-G domainDepositorInsertwbp2nlF127P
UseXenopus expressionTagsnoneExpressionMutationchanged Phenylalanine at aa127 to ProlinePromoterSP6Available sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-wbp2nlY55F
Plasmid#172466Purposeexpresses protein with one aa substitution to affect potential phosphorylation in ITSM domainDepositorInsertwbp2nlY55F
UseXenopus expressionTagsnoneExpressionMutationchanged Tyrosine at aa55 to PhenylalaninePromoterSP6Available sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-wbp2nl
Plasmid#172463Purposeexpresses wild-type protein when injected into embryosDepositorInsertwbp2nl (wbp2nl.L Frog)
UseXenopus expressionTagsnoneExpressionMutationnonePromoterSP6Available sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMCRW2
Plasmid#127380PurposepBC with 4 repeats of 80bp DNA templateDepositorInsert4 repeats of 80 bp DNA template sequence
UseTagsExpressionBacterialMutationPromoterlac promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJIT163-AFID-2
Plasmid#167358PurposeFor inducing predictable deletions in protoplasts and wheat plantsDepositorInsertA3A-Cas9-UDG
UseTagsExpressionPlantMutationWTPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-LAP1-mCherry
Plasmid#159525PurposeExpresses mCherry from LAP1 promoterDepositorInsertmCherry
UseAAVTagsExpressionMammalianMutationPromoterLAP1Available sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
Myo 9 Avi+Flag
Plasmid#134969PurposeExpression and purification of c.elegans Myo 9 (Hum 7) using baculovirus from pFastBac1DepositorInsertMyo 9 (hum-7 Nematode)
UseTagsAvi and FlagExpressionInsectMutationPromoterPolyhedrinAvailable sinceJan. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-Sh-PnGW-CMV
Plasmid#133366PurposePiggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLSDepositorInsertGFP-NLS
UseTagsHA-tagged GFPExpressionMammalianMutationN/APromoterCMVAvailable sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-ChPtW-eSpCas9
Plasmid#133381PurposePiggybac vector for constitutive expressionDepositorInserteSpCas9
UseTagsHAExpressionMammalianMutationeSpCas9 mutationsPromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-Sh-PnGW-CMV-Luc
Plasmid#133367PurposePiggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLSDepositorInsertGFP-NLS
UseTagsHA-tagged GFPExpressionMammalianMutationN/APromoterCMVAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-Sh-PIR702W-Luc
Plasmid#133362PurposePiggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLSDepositorInsertIRFP-702
UseTagsHA-tagged IRFPExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-Sh-PIR702W
Plasmid#133361PurposePiggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLSDepositorInsertIRFP-702
UseTagsHA-tagged IRFPExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-Sh-PnGW-Luc
Plasmid#133360PurposePiggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLSDepositorInsertGFP-NLS
UseTagsHA-tagged GFPExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only