Addgene

Showing: 10981 - 11000 of 24295 results

Results per page:

Sort results by:

pEF5B-FRT-AP-Smo-YFP-DEST

Plasmid

#49099

Purpose

Expressing AP-Smo-YFP in mammalian cells, can be used for establishing Flp-In stable cell lines

Depositor

Maxence Nachury

Article

Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554

Insert

Smo (Smo Mouse)

Use

Tags

YFP and acceptor peptide (AP)

Expression

Mammalian

Mutation

Promoter

T7

Available since

Nov. 8, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
Sema3e(L)-Fc-His

Plasmid

#72145

Purpose

Expresses the Sema3E protein (truncated at cleavage site P3; ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3e (Sema3e Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Ctcf-Halo-mAID donor

Plasmid

#113103

Purpose

Donor plasmid for knock-in mAID-Halo fusion to the c-terminal of mouse Ctcf coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

c terminal Ctcf donor region including homology arms and mAID-HaloTag (Ctcf Mouse, Synthetic)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
ELK4_pet28a

Plasmid

#131648

Purpose

Bacterial expression of ETS gene ELK4

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ELK4 (ELK4 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV6- Entry-RPL26DC-DDK

Plasmid

#133867

Purpose

Expression of C-terminally FLAG-tagged human RPL26 mutant lacking the C-terminal UFMylation sites

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

RPL26DC-myc-FLAG (RPL26 Human)

Use

Tags

Myc-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiV_Neo_SIK3_CR

Plasmid

#108104

Purpose

Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation) with neomycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Insert

SIK3 (SIK3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

change guanine 501 to cytosine (silent mutation)

Promoter

EFS promoter

Available since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
RSRC1

Plasmid

#155983

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

RSRC1 (RSRC1 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pWPXL-Hes1

Plasmid

#36983

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

Hes1 (Hes1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
SF3A2

Plasmid

#156001

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

SF3A2 (SF3A2 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-miR30_shBrd9_1061-PGK-NeoR-IRES-GFP

Plasmid

#75129

Purpose

Retroviral expression plasmid encoding shBrd9_1061 (targeting murine Brd9)

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

shBrd9_1061

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

May 23, 2016

Availability

Academic Institutions and Nonprofits only
pRSpuro shID3#1

Plasmid

#19163

Depositor

Joan Massague

Article

Gupta et al Proc Natl Acad Sci U S A. 2007 Dec 4. 10

Insert

small hairpin RNA against inhibitor of DNA binding 3 (ID3 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 16, 2008

Availability

Academic Institutions and Nonprofits only
pEX-CMV-SP-CFP-STM2(15-746)

Plasmid

#18863

Depositor

Tobias Meyer

Article

Brandman et al Cell. 2007 Dec 28. 131(7):1327-39.

Insert

Stromal Interaction Molecule 2 (STIM2 Human)

Use

Tags

CFP

Expression

Mammalian

Mutation

CFP inserted between signal peptide and residues …

Promoter

Available since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
ZP60 Axin2

Plasmid

#16882

Depositor

Randall Moon

Article

Weidinger et al Curr Biol. 2005 Mar 29. 15(6):489-500.

Insert

axin2 (axin2 Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
KM-CashGem-Xyl2-URA3-LINEAR

Plasmid

#174838

Purpose

pRS414 backbone containing LINEAR fragment targeting XYL2 in K. marxianus

Depositor

Zengyi Shao

Article

Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

Insert

Codon optimized Cas9:hGem

Use

CRISPR and Synthetic Biology

Tags

hGeminin tag

Expression

Bacterial and Yeast

Mutation

Promoter

TEF1p

Available since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFE-hCA

Plasmid

#132999

Purpose

pFE carrying a human carbonic anhydrase II gene (Uniprot: P00918)

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Insert

hCAII (CA2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

tetR

Available since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Psicheck2 COLIA1 full length 3'UTR

Plasmid

#26993

Depositor

Joan Massague

Article

Tavazoie et al Nature. 2008 Jan 10. 451(7175):147-52.

Insert

COL1A1 Full length 3' UTR (COL1A1 Human)

Use

Luciferase

Tags

Renilla Luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 16, 2011

Availability

Academic Institutions and Nonprofits only
pCAG-OSF-BECN1

Plasmid

#99328

Purpose

Component for mammalian expression of recombinant PI3KC3-C1 complex

Depositor

James Hurley

Article

Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

Insert

Beclin 1 (BECN1 Human)

Use

Tags

Strep-Strep-Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only
pcDNA pDEST40 Cas9-HMGB1

Plasmid

#183195

Purpose

Expression clone

Depositor

Emma Haapaniemi

Article

Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

Insert

HMGB1

Use

CRISPR

Tags

Cas9WT

Expression

Mammalian

Mutation

n/a

Promoter

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
mTagRFP-T-Actin-7

Plasmid

#58001

Purpose

Localization: Actin, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Actin (ACTB Human)

Use

Tags

mTagRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits
XE124 XDsh delta DEP-GFP-CS2+

Plasmid

#16785

Depositor

Randall Moon

Article

Miller et al J Cell Biol. 1999 Jul 26;146(2):427-37.

Insert

Xdsh-delta-DEP (dvl2.L Frog)

Use

Xenopus expression

Tags

GFP

Expression

Mutation

contains Dsh amino acids 1-433 and 503-736 (delet…

Promoter

Available since

March 14, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge