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Plasmid#83476PurposeExpresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains KanMx4 marker for yeast transformation.DepositorInsertKanMX4
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUDP293
Plasmid#204228PurposeEpisomal yeast plasmid expressing the Ercas12a and ErCas12a gRNADepositorInsertsEubacterium rectale cas12a (Ercas12a)
BsaI_GFP drop-out_BsaI
UseSynthetic BiologyTagsNLSExpressionMutationcodon optimized for S. cerevisiaePromoterSaccharomyces cerevisiae PGK1 and Saccharomyces c…Available sinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTrcHisB-TRF1
Plasmid#53209PurposeExpressed N-terminally Hexahistidine tagged TRF1 proteinDepositorInsertTRF1 (TERF1 Human)
UseTagsHexahistidineExpressionBacterialMutation3-439PromoterTrcAvailable sinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTP264
Plasmid#149334PurposeBacterial expression of Biotin ligase BirADepositorInsertBiotin ligase BirA
UseTagsHis14 and bdNEDD8ExpressionBacterialMutationPromoterAvailable sinceJune 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
CamKII-LGI1-pHluorin
Plasmid#185537PurposeExpresses LGI1-pHluorin under the CamKII promoterDepositorInsertLGI1 (Lgi1 Rat)
UseLentiviralTagspHluorinExpressionMammalianMutationPromoterCamKIIAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF0608
Plasmid#142659PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMAFA (MAFA Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-cMYC promoter
Plasmid#114724Purposereporter luciferase constructDepositorInsertC-Myc promoter region
UseTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMuLE_EXPR_CMV-eGFP_TOP-NLuc1.1_12GLI-FLuc_CBF-GLuc
Plasmid#113862PurposeTriple pathway reporter, 3P-Luc; wnt-NLuc1.1, hedgehog-FLuc, notch-GLuc; plus CMV-eGFP. Gateway expression vector for lentivirus generation.DepositorInsertsCMV-eGFP
TOP-NLuc1.1
12GLI-Fluc
CBF-GLuc
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJF143.J3
Plasmid#153028PurposeJ3-mRFP1DepositorInsertJ3-mRFP1
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-HA-deLACCO1-NGR
Plasmid#208097PurposeControl biosensor for extracellular L-lactateDepositorInsertdeLACCO1
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only