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Plasmid#86248PurposeFor generating transgenic lines that ubiquitously expressing the fluorescent MCP reporterDepositorInsertbeta Actin promoter:NLS-tdMCPeGFP
UseUsed with transposase to make transient or stable…TagsNLS-tdMCP-eGFPExpressionMutationPromoterBeta ActinAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1656
Plasmid#51483PurposeminiMos transposable element with HygroR and peel-1, R4-R3 Gateway cloningDepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceFeb. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1666
Plasmid#51485PurposeminiMos transposable element with PuroR, MCS cloningDepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceMarch 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-hUbC-EGFP-sgRNA.FOXA1/A2/A3_CRISPRi
Plasmid#216166PurposeExpress the gRNAs for the human FOXA1/A2/A3-CRISPRiDepositorInsertUseLentiviralTagsEGFPExpressionMutationPromoterphU6, ph7SK, phH1, pmU6Available sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUC18_BCR_GUSB_e6a2
Plasmid#190882Purposemolecular monitoring of atypical BCR::ABL1 fusion transcripts (e6a2)DepositorUseCloned cdna transcripts for use in rt-qpcr bcr::a…TagsExpressionMutationPromoterAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAB1
Plasmid#62547Purposebroad host range plasmid for IPTG-inducible expression of eGFP. Designed for imaging Pseudomonas aeruginosa activity in biofilms.DepositorInserteGFP
UseBroad host range plasmidTagsExpressionBacterialMutationPromoterpTrcAvailable sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pDJSVT86
Plasmid#108965PurposeExpress periplasmic, outer membrane, or secreted proteins in E. coli - OmpA signal and 6His tagDepositorTypeEmpty backboneUseTagsOmpA 1-27, 6HisExpressionBacterialMutationPromoterT7Available sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMS29: pDonor(PmcCAST)
Plasmid#168162PurposePmcCAST donor harboring non-functional cargo.DepositorInsertsPmcCAST right end (RE)
PmcCAST left end (LE)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS30: pTarget(PmcCAST)
Plasmid#168163PurposeTarget plasmid containing PmcPSP1 and attachment site for PmcCAST.DepositorInsertsPAM for PmcCAST + PmcPSP1
tRNA-Val
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEINO4
Plasmid#179488PurposeMulti-host shuttle plasmid for E. coli, D. radiodurans, and S. cerevisiae; contains an oriT for transfer via conjugationDepositorInsertaadA1
UseSynthetic Biology; Bacterial and yeast cloningTagsExpressionMutationPromoterAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMW37: pRS426-R2Bm RNA
Plasmid#209291PurposeContains the R2Bm non-LTR retrotransposon 5'UTR and 3'UTR sequencesDepositorInsertR2Bm 5'UTR and 3'UTR
UseTagsExpressionBacterial and YeastMutationPromoterGAL1 GAPDHAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMS32: pTwinStrep-SUMO-PmcTnsC
Plasmid#168165PurposePmcTnsC expression construct for protein purification.DepositorInsertPmcCAST TnsC
UseTags6X His, Twin Strep and SUMOExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
TRAPa-3xFLAG-pCDNA
Plasmid#201963PurposeMammalian expression of Translocon-associated protein subunit alphaDepositorInsertSSR1 (SSR1 Human)
UseTags3xFLAG-tagExpressionMammalianMutationPromoterAvailable sinceJan. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMS31: pTwinStrep-SUMO-PmcTnsAB
Plasmid#168164PurposePmcTnsAB expression construct for protein purification.DepositorInsertPmcCAST TnsAB
UseTags6X His, Twin Strep and SUMOExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAL612
Plasmid#119860Purposeprecusor outer membrane protein A, ompA, with mutations Cysteine 290 Serine, Glycine 244 CysteineDepositorInsertouter membrane protein A, ompA, with mutations Cysterine 290 to Serine and Glycine 244 to Cysteine
UseTagsnoneExpressionBacterialMutationCysterine 290 to Serine and Glycine 244 to Cystei…PromoterlacAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAL725
Plasmid#119862Purposeprecusor D-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287CDepositorInsertD-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287C (mglB Escherichia coli str. K-12)
UseTagsnoneExpressionBacterialMutationLeucine 244 to Cysteine, Aspartate 287 to CysteinePromoterT5Available sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBGSA FPR1
Plasmid#62600PurposeConstitutive expression of FPR1 in mammalian cellsDepositorInsertFPR1 (FPR1 Human)
UseTagsExpressionMammalianMutationPromoterSRalphaAvailable sinceFeb. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
TRAPa(R150A)-3xFLAG-pCDNA
Plasmid#201966PurposeMammalian expression of mutated Translocon-associated protein subunit alphaDepositorInsertSSR1 (SSR1 Human)
UseTags3xFLAG-tagExpressionMammalianMutationR150APromoterAvailable sinceJan. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTA-Mob 2.0
Plasmid#149662PurposeConjugation helper plasmid for use in cis (self-transfer) and trans (transfer of plasmids with RK2/RP4 origin of transfer) mobilization to prokaryotic and eukaryotic cells.DepositorTypeEmpty backboneUseSynthetic Biology; Conjugation helper plasmidTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only