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LV-PGK-PVR-P2A-Cre-EFS-mScarletSIIN

Plasmid

#172435

Purpose

Lentivirus, expresses Cre recombinase, mouse PVR (CD155), and mScarlet-SIINFEKL

Depositor

Tyler Jacks

Article

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Inserts

Cre
PVR
mScarlet-SIINFEKL(OVA257-264)+Ova 323-339

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABE8.20m-SpCas9-NRCH-P2A-EGFP (RMD55)

Plasmid

#197501

Purpose

CMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRCH(D10A) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

Insert

pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRCH-BPNLS-3xFLAG-P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS and BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

ABE8.20 mutations in TadA and nSpCas9-NRCH(D10A/I…

Promoter

CMV and T7

Available since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABE8.20m-SpCas9-NRTH-P2A-EGFP (RMD63)

Plasmid

#197500

Purpose

CMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRTH(D10A) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

Insert

pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRTH-BPNLS-3xFLAG-P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS and BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

ABE8.20 mutations in TadA and nSpCas9-NRTH(D10A/I…

Promoter

CMV and T7

Available since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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XE92 XGCNF-EnR

Plasmid

#17029

Depositor

Randall Moon

Article

Song et al Dev Biol. 1999 Sep 1;213(1):170-9. doi:

Insert

XGCNF-EnR (en Fly)

Use

Xenopus expression

Tags

Expression

Mutation

Promoter

Available since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
CAG-EnR

Plasmid

#19715

Depositor

Connie Cepko

Article

Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

Insert

engrailed (en Fly)

Use

Tags

Expression

Mammalian

Mutation

Drosophila engrailed repressor domain

Promoter

Available since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
pLIA EnR (CC#29)

Plasmid

#15152

Depositor

Connie Cepko

Article

Furukawa et al Cell. 1997 Nov 14. 91(4):531-41.

Insert

Engrailed repressor (en Fly)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

engrailed repressor domain

Promoter

Available since

June 15, 2007

Availability

Academic Institutions and Nonprofits only
KHBD00219

Plasmid

#34367

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG9015 (en Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
TetO-FUW-MCS-EnR_C-term

Plasmid

#136885

Purpose

Doxycycline-inducible vector backbone containing a multiple cloning site (MCS), the engrailed (EnR) repressor domain and a STOP codon

Depositor

Moritz Mall

Article

Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

Insert

engrailed repressor domain (en Fly)

Use

Lentiviral

Tags

None

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-FLAG-NLS-EnR-MCS_N-term

Plasmid

#136884

Purpose

Doxycycline-inducible vector backbone containing the engrailed (EnR) repressor domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)

Depositor

Moritz Mall

Article

Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

Insert

engrailed repressor domain (en Fly)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-haA3A-CBE-A-NG

Plasmid

#220895

Purpose

Express haA3A-CBE-A-NG (with SpCas9-NG variant) in mammalian cells

Depositor

Dali Li

Article

Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

Insert

haA3A-CBE-A-NG

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-haA3A-CBE-G-N

Plasmid

#220898

Purpose

AAV genome encoding the N-terminal of haA3A-CBE-G

Depositor

Dali Li

Article

Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

Insert

haA3A-CBE-G-N

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-haA3A-CBE-VA

Plasmid

#220893

Purpose

Express haA3A-CBE-VA (with SpCas9) in mammalian cells

Depositor

Dali Li

Article

Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

Insert

haA3A-CBE-VA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-haA3A-CBE-A

Plasmid

#220892

Purpose

Express haA3A-CBE-A (with SpCas9) in mammalian cells

Depositor

Dali Li

Article

Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

Insert

haA3A-CBE-A

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-haA3A-CBE-G-NG

Plasmid

#220897

Purpose

Express haA3A-CBE-G-NG (with SpCas9-NG variant) in mammalian cells

Depositor

Dali Li

Article

Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

Insert

haA3A-CBE-G-NG

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-haA3A-CBE-VA-NG

Plasmid

#220896

Purpose

Express haA3A-CBE-VA-NG (with SpCas9-NG variant) in mammalian cells

Depositor

Dali Li

Article

Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

Insert

haA3A-CBE-VA-NG

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-haA3A-CBE-G

Plasmid

#220894

Purpose

Express haA3A-CBE-G (with SpCas9) in mammalian cells

Depositor

Dali Li

Article

Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

Insert

haA3A-CBE-G

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-haA3A-CBE-G-C

Plasmid

#220899

Purpose

AAV genome encoding the C-terminal of haA3A-CBE-G, and sgRNA

Depositor

Dali Li

Article

Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

Insert

haA3A-CBE-G-C

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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TMEM230-WT-pEGFP(isoform 2)

Plasmid

#99562

Purpose

mammalian expression of TMEM230-GFP (isoform 2)

Depositor

Han-Xiang Deng

Article

Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

Insert

TMEM230 (TMEM230 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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TMEM230-WT-pEGFP(isoform 1)

Plasmid

#99561

Purpose

mammalian expression of TMEM230-GFP (isoform 1)

Depositor

Han-Xiang Deng

Article

Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

Insert

TMEM230 (TMEM230 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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TMEM230-WT-IRES(isoform 2)

Plasmid

#99564

Purpose

mammalian expression of TMEM230 (isoform 2) and ZsGreen

Depositor

Han-Xiang Deng

Article

Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

Insert

TMEM230 (TMEM230 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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