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Addgene
Showing: 1081 - 1100 of 4601 results

  1. pPB-spCas9-pNeo

    Plasmid
    #214120
    Purpose
    Gene expression by Piggybac transposase in mammalian cells
    Depositor
    Sho Yoshimatsu
    Insert
    spCas9
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    214120-plasmid-map-sequence-id-423617

    Enlarge

  2. pPB-spCas9-pZeo

    Plasmid
    #214121
    Purpose
    Gene expression by Piggybac transposase in mammalian cells
    Depositor
    Sho Yoshimatsu
    Insert
    spCas9
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    214121-plasmid-map-sequence-id-423619

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  3. pMB22.SV40.Zeo

    Plasmid
    #135692
    Purpose
    Lentivirus delivery for stable expression of AsCas12a-VPR, Zeocin selection
    Depositor
    Florian Karreth
    Insert
    mammalian codon-optimized Acidaminococcus sp. Cas12a fused to VPR activator
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    135692-plasmid-map-sequence-id-322223

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  5. pPB-CAG-PlatTET-wpA-pPuroTK

    Plasmid
    #214125
    Purpose
    Gene expression by Piggybac transposase in mammalian cells
    Depositor
    Sho Yoshimatsu
    Insert
    dCas9-TET
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    214125-plasmid-map-sequence-id-424237

    Enlarge

  6. pCDB179-TfCut2-S121P-D174S-D204P

    Plasmid
    #218695
    Purpose
    Encodes codon optimized TfCut2-S121P-D174S-D204P enzyme for bacterial expression
    Depositor
    Gregg Beckham
    Insert
    TfCut2
    Use
    Tags
    10xHis
    Expression
    Bacterial
    Mutation
    S121P-D174S-D204P
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    218695-plasmid-map-sequence-id-434086

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  7. pCDB179-TfCut2-L32E-S113E-T237Q

    Plasmid
    #218696
    Purpose
    Encodes codon optimized TfCut2 L32E-S113E-T237Q enzyme for bacterial expression
    Depositor
    Gregg Beckham
    Insert
    TfCut2
    Use
    Tags
    10xHis
    Expression
    Bacterial
    Mutation
    L32E-S113E-T237Q
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    218696-plasmid-map-sequence-id-434085

    Enlarge

  8. pCDB179-PES-H1-L92F-Q94Y

    Plasmid
    #218705
    Purpose
    Encodes codon optimized PES-H1 L92F-Q94Y enzyme for bacterial expression
    Depositor
    Gregg Beckham
    Insert
    PES-H1
    Use
    Tags
    10xHis
    Expression
    Bacterial
    Mutation
    L92F-Q94Y
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    218705-plasmid-map-sequence-id-437310

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  9. pME-dCas9-BFP-KRAB

    Plasmid
    #221502
    Purpose
    CRISPRi machinery fish codon optimized
    Depositor
    Alessandro Ori
    Insert
    dCas9-tagBFP-KRAB
    Use
    Fish expression
    Tags
    Expression
    Mutation
    D10A and H839A point mutations
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    221502-plasmid-map-sequence-id-439413

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  13. hSyn1-FLEX-hChR2-tdtomato

    Plasmid
    #41015
    Depositor
    Karel Svoboda
    Insert
    Channelrhodopsin-2
    Use
    Adenoviral
    Tags
    tdtomato
    Expression
    Mammalian
    Mutation
    codon-optimized (human)
    Promoter
    synapsin
    Availability
    Academic Institutions and Nonprofits only

  14. pH6-TEV-mCherry

    Plasmid
    #132430
    Purpose
    Expresses His6-tagged mCherry in E. coli using T7 promoter
    Depositor
    Mark Arbing
    Insert
    mCherry
    Use
    Tags
    N-terminal His6 tag with TEV protease site
    Expression
    Bacterial
    Mutation
    Codon optimized
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    132430-plasmid-map-sequence-id-258769

    Enlarge

  15. pET15-mFAP10

    Plasmid
    #159463
    Purpose
    Encodes for mFAP10 with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.
    Depositor
    David Baker
    Insert
    mFAP10
    Use
    Tags
    6xHis-thrombin-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    159463-plasmid-map-sequence-id-316643

    Enlarge

  16. pET15-EF4n_mFAP2b

    Plasmid
    #159464
    Purpose
    Encodes for EF4n_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.
    Depositor
    David Baker
    Insert
    EF4n_mFAP2b
    Use
    Tags
    6xHis-thrombin-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    159464-plasmid-map-sequence-id-316655

    Enlarge

  17. pET15-EF2n3_mFAP2b

    Plasmid
    #159466
    Purpose
    Encodes for EF2n3_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.
    Depositor
    David Baker
    Insert
    EF2n3_mFAP2b
    Use
    Tags
    6xHis-thrombin-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    159466-plasmid-map-sequence-id-319724

    Enlarge

  18. pET15-EF2n2_mFAP2b

    Plasmid
    #159467
    Purpose
    Encodes for EF2n2_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.
    Depositor
    David Baker
    Insert
    EF2n2_mFAP2b
    Use
    Tags
    6xHis-thrombin-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    159467-plasmid-map-sequence-id-316656

    Enlarge

  19. pET15-EF2n_mFAP2b

    Plasmid
    #159468
    Purpose
    Encodes for EF2n_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.
    Depositor
    David Baker
    Insert
    EF2n_mFAP2b
    Use
    Tags
    6xHis-thrombin-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    159468-plasmid-map-sequence-id-316657

    Enlarge

  20. pET15-EF1p_mFAP2b

    Plasmid
    #159469
    Purpose
    Encodes for EF1p_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.
    Depositor
    David Baker
    Insert
    EF1p_mFAP2b
    Use
    Tags
    6xHis-thrombin-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    159469-plasmid-map-sequence-id-319725

    Enlarge

Showing: 1081 - 1100 of 4601 results