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pMJ45: pRS426-His-MBP-SpuFz1_Native

Plasmid

#205252

Purpose

S. cerevisiae expression vector for SpuFz1 native guide

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Use

Tags

10xHis, MBP

Expression

Yeast

Mutation

Promoter

Available since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pMJ123: pRS426-His-MBP-SpuFz1_PSP1

Plasmid

#205256

Purpose

S. cerevisiae expression vector for SpuFz1 reprogrammed guide (targeting PSP1)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Use

Tags

10xHis, MBP

Expression

Yeast

Mutation

Promoter

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMJ240: pRS426-His-MBP-MmeFz2_Native

Plasmid

#205255

Purpose

S. cerevisiae expression vector for MmeFz2 native guide

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

MmeFz2

Use

Tags

10xHis, MBP

Expression

Yeast

Mutation

Promoter

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMJ235: pRS426-His-MBP-GtFz1_Native

Plasmid

#205254

Purpose

S. cerevisiae expression vector for GtFz1 native guide

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

GtFz1

Use

Tags

10xHis, MBP

Expression

Yeast

Mutation

Promoter

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMJ273: pRS426-His-MBP-GtFz1_PSP1

Plasmid

#205258

Purpose

S. cerevisiae expression vector for GtFz1 reprogrammed guide (targeting PSP1)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

GtFz1

Use

Tags

10xHis, MBP

Expression

Yeast

Mutation

Promoter

Available since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pMJ127: pRS426-His-MBP-NlovFz2_PSP1

Plasmid

#205257

Purpose

S. cerevisiae expression vector for NlovFz2 reprogrammed guide (targeting PSP1)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

NlovFz2

Use

Tags

10xHis, MBP

Expression

Yeast

Mutation

Promoter

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pFRTpsiCHECK_Gaussia_luciferase

Plasmid

#154459

Purpose

Expression of gaussia luciferase and firefly luciferase

Depositor

Markus Landthaler

Article

Zinnall et al Nat Commun. 2022 May 18;13(1):2727. doi:

Insert

Gaussia luciferase

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-iLACCO1

Plasmid

#218821

Purpose

Mammalian expression of genetically encoded green fluorescent L-lactate biosensor with mild affinity

Depositor

Robert Campbell

Article

Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

Insert

iLACCO1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-DiLACCO1

Plasmid

#218824

Purpose

Mammalian expression of genetically encoded green fluorescent L-lactate control biosensor

Depositor

Robert Campbell

Article

Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

Insert

DiLACCO1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST exorh:mCherry

Plasmid

#195989

Purpose

Transgenesis backbone

Depositor

Nicolas Rohner, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCK017 pDEST exorh:EGFP

Plasmid

#195983

Purpose

Transgenesis backbone

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-iLACCO1.2

Plasmid

#218823

Purpose

Mammalian expression of genetically encoded green fluorescent L-lactate biosensor with high affinity

Depositor

Robert Campbell

Article

Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

Insert

iLACCO1.2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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pCK054 pDEST exorh:mCerulean

Plasmid

#195985

Purpose

Transgenesis backbone

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Other

Tags

Expression

Mutation

Promoter

Available since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCINeo-myc-Itch/2859

Plasmid

#11427

Depositor

Allan Weissman

Article

Magnifico et al J Biol Chem. 2003 Oct 31. 278(44):43169-

Insert

Itch (Itch Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

n/a

Promoter

Available since

April 5, 2006

Availability

Academic Institutions and Nonprofits only

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TERTpWT_Gluc_CCR5

Plasmid

#192270

Purpose

Donor template for insertion of hTERT promoter driven Gaussia luciferase reporter

Depositor

Shantanu Chowdhury

Article

Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

Insert

Promoter Reporter Insert within donor template

Use

CRISPR

Tags

Expression

Mutation

Promoter

TERT promoter driving expression of gaussia

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pGL3-Promoter-4xBS_BHLHE40

Plasmid

#153064

Purpose

Four BHLHE40 consensus binding sites cloned upstream of luciferase reporter gene

Depositor

Silvia Monticelli

Article

Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

Insert

4X consensus BHLHE40 binding sites

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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pLPCX2-FLAG-Smad4

Plasmid

#89834

Purpose

Express Smad4 in mammalian cells

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

Insert

SMAD4 (SMAD4 Human)

Use

Retroviral

Tags

FLAG

Expression

Mutation

Promoter

CMV

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST exorh:mGreenLantern

Plasmid

#195988

Purpose

Transgenesis backbone

Depositor

Nicolas Rohner, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-GFP-EBA175-Flag

Plasmid

#154970

Purpose

Expresses N-terminally GFP-tagged EBA175 (residues 1284-1462) from pcDNA3.1. Please note: Plasmid contains a Stop codon prior to the C-terminal Flag tag.

Depositor

Sinisa Urban

Article

Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

Insert

PfEBA175

Use

Tags

GFP and signal peptide (residues 1 to 32) from TG…

Expression

Mammalian

Mutation

insert codes for EBA175 residues 1284 to 1462

Promoter

CMV

Available since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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BAT-gal

Plasmid

#20889

Depositor

Stefano Piccolo

Article

Maretto et al Proc Natl Acad Sci U S A. 2003 Mar 18. 1

Insert

7XTCF binding sites, Siamois minimal promoter, NLS-lacz

Use

Mouse Targeting

Tags

Expression

Mammalian

Mutation

7 multimerized TCF-binding elements cloned upstre…

Promoter

Available since

May 7, 2009

Availability

Academic Institutions and Nonprofits only