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pIND4-GFP-MmXYRS
Plasmid#222745PurposeExpresses sfGFP and aaRS-tRNA pair for incorporating haloalkane crosslinkerDepositorInsertssfGFP
MmXYRS
Mm Pyl tRNA
UseTags6xHisExpressionMutationContains the following substitutions: V346A, W348…PromoterrrnBAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pECNUS2
Plasmid#184885PurposeABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA deliveryDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterRPS5AAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
Minos ITR loxp MCS lox2272
Plasmid#218983PurposePlasmid containing ITR sequences compatible with Minos transposase and multiple cloning siteDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-DDX53-GFP
Plasmid#222239PurposeExpress GFP tagged DDX53 in mammalian cellsDepositorInsertDDX53 (DDX53 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMV and T7Available sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMV-dCas9_puroR
Plasmid#219825PurposeCMV-driven expression of dCas9 with puromycin resistance (Adapted from plasmid #68416)DepositorInsertdCas9
UseCRISPRTagsExpressionMammalianMutationD10A/H841APromoterCMVAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXR004_puroR
Plasmid#219820PurposehU6-driven pre-gRNA plasmid for CasRx applications with puromycin resistance. 5' processed DR followed by BbsI sites for guide cloning (Adapted from plasmid #10954)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
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Lmod1-BirA
Plasmid#221509PurposeFlag-tagged biotinylation enzyme for co-expression of Lmod1DepositorInsertLmod1 (Lmod1 Mouse)
UseTagsBirAExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
SYNJ1_BirA_Nterm
Plasmid#221523PurposeFlag-tagged biotinylation enzyme for co-expression of Synj1DepositorInsertSYNJ1 (SYNJ1 Human)
UseTagsBirAExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
SYNJ1_BirA_Cterm
Plasmid#221522PurposeFlag-tagged biotinylation enzyme for co-expression of Synj1DepositorInsertSYNJ1 (SYNJ1 Human)
UseTagsBirAExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJ1-g1g2-K2
Plasmid#218217PurposeAllows users to clone two Spacer sequences into an arrray of AtU6-promoter driven sgRNAs, MoClo compatibleDepositorInsertAtU6 promoters and sgRNA scaffolds
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only
