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Plasmid#209869PurposeDimerization dependent fluorescent protein RA anchored to cytosolic face of the peroxisomal membrane with N-terminal targeting sequence of human PEX3DepositorInsertddRFP A
UseTagsTargeting domain of PEX3 MLRSVWNFLKRHKKKCIFLGTVLG…ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 GATA1
Plasmid#85693PurposeMammalian expression of mouse GATA1DepositorInsertGATA1 (Gata1 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-CHD7
Plasmid#170141PurposeExpresses CHD7 in insect cellsDepositorInsertchromodomain helicase DNA binding protein 7 (CHD7 Human)
UseTags6xHis and FLAGExpressionInsectMutationPromoterAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJRoC30_VP8H
Plasmid#188062PurposePROSS-designed versatile peroxidase 8HDepositorInsertPROSS-designed versatile peroxidase from Ganoderma sp. 10597_SS1
UseTagsS. cerevisiae native α-factor prepro-leader signa…ExpressionYeastMutation38 PROSS mutations, described in publicationPromoterAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-APEX.NES-P2A-EGFP
Plasmid#182824PurposeCre-dependent expression of cytosolic APEXDepositorInsertAPEX.NES-P2A-EGFP
UseAAVTagsEGFP reporter (P2A)ExpressionMutationPromoterEF1aAvailable sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLR16-Phes*
Plasmid#98783PurposeSuicide vector for allelic exchange in Listeria utilizing an engineered counterselection marker for Listeria monocytogenes based on a mutated phenylalanyl-tRNA synthetase gene (pheS*).DepositorInsertpheS
UseTagsExpressionBacterialMutationA309GPromoterAvailable sinceJan. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLostfoX-Kan
Plasmid#99865PurposeTfoX induction vector for chitin-pathway transformation (KanR)DepositorInserttfoXVF
UseV. fischeri suicide cloning vector, cmr, tcrTagsExpressionMutationPromoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYFJ16-LplA(W37V) (for E. Coli expression)
Plasmid#34838DepositorInsertLplA W37V (lplA E. Coli)
UseTagsHis TagExpressionBacterialMutationMutated Trp37 to ValPromoterT5 promoterAvailable sinceMay 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJB556
Plasmid#115966PurposeSGP insert for replicon MoCloDepositorInsertLvl0 -98/15 SGP 2xTetApt-STOP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
scFLARE1
Plasmid#158738PurposeMammalian expression of scFLARE1 componentDepositorInsertscFLARE1
UseAAVTagsFlag, V5ExpressionMammalianMutationTruncated form of TEV protease (220-242 amino aci…PromoterCMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
Perox-GA
Plasmid#209868PurposeDimerization dependent fluorescent protein GA anchored to cytosolic face of the peroxisomal membrane with N-terminal targeting sequence of human PEX3DepositorInsertddGFP A
UseTagsTargeting domain of PEX3 MLRSVWNFLKRHKKKCIFLGTVLG…ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAT9650-BEAR-GFP-preedited
Plasmid#162992PurposeBEAR control plasmid with split EGFP and intact 5' splice siteDepositorInsertEGFP split with an intron between amino acids 95-96
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
hHS1
Plasmid#159169PurposeHuman codon-optimized cytosolic labile heme reporterDepositorInserthHS1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
V5-LOV-Turbo1-G3BP1_pTRE-G418
Plasmid#202015Purposeexpresses LOV-Turbo1-G3BP1 in the mammalian cytosol under dox inducible promoter, lentiviral vectorDepositorInsertV5-LOV-Turbo1-G3BP1
UseLentiviralTagsV5ExpressionMammalianMutationPromoterpTRE-TightAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
MBP-TEVcs(ENLYFQ/G)-His6-uTEV2Δ(220-242)-R5
Plasmid#135456PurposeBacterial expression of uTEV2Δ proteaseDepositorInsertMBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5
UseTagsMBP, His6ExpressionBacterialMutationS219V mutation improves stability, T30A/S153N mut…Promotertac promoterAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-RRBP1-1
Plasmid#92156PurposeCRISPR guide RNA targeting human RRBP1DepositorInsertRRBP1 sgRNA-1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
V5-LOV-Turbo-ERM
Plasmid#199664Purposeexpresses LOV-Turbo on the mammalian ER membrane, lentiviral vectorDepositorInsertLOV-Turbo
UseLentiviralTagsSEC61B and V5ExpressionMammalianMutationPromoterTRE3GAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSEM272 - MCS mosTI Pmlc-2_GFP target
Plasmid#159834PurposeSingle copy or array insertion by MosTI using split Pmlc-2 GFP selectionDepositorInsertMCS mosTI Pmlc-2_GFP target
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
V5-LOV-Turbo-NLS
Plasmid#199663Purposeexpresses LOV-Turbo in the mammalian nucleus, lentiviral vectorDepositorInsertLOV-Turbo
UseLentiviralTagsNLS and V5ExpressionMammalianMutationPromoterTRE3GAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH-hSyn-NCreIntN-T2A-mTagBFP2
Plasmid#160502PurposeExpresses NCreIntN in neurons in split intein-mediated split-Cre recombinase applicationsDepositorInsertNCreIntN
UseCre/Lox and LentiviralTagsmTagBFP2ExpressionMammalianMutationPromoterhSynAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only