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Plasmid#148496PurposeMammalian Expression of HsMex3CDepositorInsertHsMex3C (MEX3C Human)
UseTagsExpressionMammalianMutationone non silent mutation compared to variant 2 fro…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
RFXANK:JMC029-A02:C213521
Plasmid#183980PurposeBaculovirus expression for structure determination. May not contain entire coding region of gene.DepositorInsertRFXANK (RFXANK Human)
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
MPP1:HPC09T-D06:C210522
Plasmid#183976PurposeBaculovirus expression for structure determination. May not contain entire coding region of gene.DepositorInsertMPP1 (MPP1 Human)
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
FNF-CAG-L-3xSV40pA-L-tdTomato shuttle vector
Plasmid#182346PurposePacI-flanked CAG-LSL expression cassette with a FNF for positive selectionDepositorInserttdTomato
UseMouse Targeting; Flp / frtTagsExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-HsRCK_296-472(MCSI)_B
Plasmid#145971PurposeBacterial Expression of HsRck_296-472DepositorInsertHsRck_296-472 (DDX6 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
SDCBPA-c021
Plasmid#175485PurposeProtein expression in bacterial cells. PDZ1 and PDZ2 domains (truncated at C-terminus), G111-F275.DepositorInsertSDCBP (SDCBP Human)
UseTagsHis6-TEVExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
SDCBPA-c011
Plasmid#175483PurposeProtein expression in bacterial cells. PDZ1 and PDZ2 domains (truncated at C-terminus), A106-F275.DepositorInsertSDCBP (SDCBP Human)
UseTagsHis6-TEVExpressionBacterialMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDX4-donor
Plasmid#176343PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertCDX4 (CDX4 Human)
UseCRISPRTagsGFPExpressionMutationPromoterAvailable sinceNov. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJAZF1-donor
Plasmid#176352PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertJAZF1 (JAZF1 Human)
UseCRISPRTagsGFPExpressionMutationPromoterAvailable sinceNov. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pB2HoptX
Plasmid#163832PurposeInactive two-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and PTP1BDepositorInsertSpecR
UseSynthetic BiologyTagsExpressionBacterialMutationMidT Substrate Y4FPromoterPro1;ProD;pLacZ-OptAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBK CUP1 mCherry
Plasmid#170264PurposeYeast two-hybrid vector using a copper inducible CUP1 promoter, expressing mCherryDepositorArticleInsertmomeric Cherry
UseTagsGAL4 DNA Binding Domain and MycExpressionBacterial and YeastMutationPromoterCUP1Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHS29
Plasmid#171205Purposebacterial expression for HIS purification of MEG-3 with mutations in the HMG-like motifDepositorInsertmeg-3 (meg-3 Nematode)
UseTags6xHISExpressionBacterialMutationF705A,Y708A,Y713A, N725APromoterAvailable sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
HCP4
Plasmid#166106PurposeThis plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the center of Cyr1 and the other targets a non-coding region on chromosome X (location X1)DepositorInsertCyr1Del-sgRNA/X1-sgRNA (CYR1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
HCP6
Plasmid#166108PurposeThis plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the C-terminus of Cyr1 and the other targets a non-coding region on chromosome X (location X1)DepositorInsertCyr1CT-sgRNA/X1-sgRNA (CYR1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
C-BRPF3-8
Plasmid#166516PurposescFv of a human scaffold targeting Bromodomain and PHD finger-containing protein 3. Antigen coverage aa 1056-1195 of 1205, PWWP domain.DepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialMutationPromoterLacZAvailable sinceMarch 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGB3_alpha1 gRNAs 2.1 P35S -104 -162 -229 (GB2759)
Plasmid#160631PurposeMultiplex construct with 2.1 aptamer in scf to target -104 -162 -229 positions of 35s promoter.DepositorInsertgRNA
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterU626Available sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDGB3 omega1 Tnos:nptII:Pnos - SF - P35S:hCas9:Tnos (GB1657)
Plasmid#160640PurposeModule for the expression of the human codon optimized Cas9 and kanamycin resistance (NptII).DepositorInsertTnos:NptII:Pnos-SF-P35s:hCas9:tNos
UseCRISPRTagsExpressionMutationBsaI and BsmBI sites removedPromoterPnos, 35SAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pEPYC0CM0182
Plasmid#154609PurposePhytobricks Level 0 partDepositorInsertMinSyn_046
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only