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Plasmid#195194Purposedual gRNAs for deletion of OR2M4 in a third generation Cas9 backbone with GFPDepositorInsertOR2M4 dual gRNA (OR2M4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTMB400_ZFcharm_Prnp_DPM
Plasmid#220847PurposeAAV genome expressing ZFcharm targeting Prnp; double perfect match self-silencing binding siteDepositorInsertZFcharm
UseAAVTagsHAtagExpressionMammalianMutationN/APromoterEFSAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTMB399_ZFcharm_Prnp_SPM
Plasmid#220846PurposeAAV genome expressing ZFcharm targeting Prnp; single perfect match self-silencing binding siteDepositorInsertZFcharm
UseAAVTagsHAtagExpressionMammalianMutationN/APromoterEFSAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTMB340_ZFcharm_Kv1_Prnp_DPM
Plasmid#220843PurposeAAV genome expressing ZFcharm Kv1 targeting Prnp; double perfect match self-silencing binding siteDepositorInsertZFcharm Kv1
UseAAVTagsHAtagExpressionMammalianMutationN/APromoterEFSAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-sumo-NSP12 (SARS-CoV-2)
Plasmid#159107PurposeThe SARS-CoV-2 NSP12 coding sequence was cloned into a modified pRSFDuet-1 vector (Novagen) bearing an N-terminal His6-SUMO-tag which is cleavable by the ubiquitin-like protease (ULP1).DepositorInsertNSP12
UseTagsHis-SUMO tagExpressionBacterialMutationNonePromoterT7Available sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMVd1-NLS-GAL4-iLight-VP16-T2A-mTagBFP2
Plasmid#170273PurposeNLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2 construct expression in mammalian cells.DepositorInsertNLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2
UseTagsExpressionMammalianMutationSee CommentsPromoterCMVd1Available sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG-MAVS
Plasmid#158628PurposeGateway entry vector for an inducible 3xFLAG-tagged MAVSDepositorInsertMAVS (MAVS Human)
UseGateway entry vectorTags3XFLAGExpressionMutationPromoterAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-VPR-S-L1-dCjCas9 MiniCAFE
Plasmid#169910PurposeExpression of a truncated VPR-dCjCas9 fusion protein to activate gene expression.DepositorInsertMiniCAFE
UseCRISPRTagsExpressionMammalianMutationD8A, HNH-truncation (Δ495–609 aa) in CjCas9PromoterCMVAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVNP3.0-PEmaxRH
Plasmid#206884PurposeExpresses FLAG-tagged PEmax (lacking the RNAseH domain) fused to Gag through a linker sequenceDepositorInsertPEMax Delta RNAseH
UseTagsFLAGExpressionMutationPromoterCMVAvailable sinceOct. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLT 653
Plasmid#59996PurposeExpresses a hybrid dsRNA corresponding to the Caenorhabditis elegans dhc-3/klp-16 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progenyDepositorUseTagsExpressionBacterialMutationpartial genomicPromoterAvailable sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/T0-Flag-53BP1
Plasmid#52507Purposemammalian expression vector of 53BP1DepositorInsert53BP1 (TP53BP1 Human)
UseTagsFlagExpressionMammalianMutationV128A and siRNA resistant sequence: ACGCGCTGACGAC…PromoterCMV/TOAvailable sinceMarch 26, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pGEX Stat3-SH2
Plasmid#46513DepositorInsertStat3 (STAT3 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 584-700PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pENT-L1-Kozak-Ensconsin-3XGFP-stop-L2
Plasmid#186361PurposeEntry clone with ORF encoding the MT-binding domain of Ensconsin fused to 3 copies of GFP in C-terminal, flanked by Gateway recombination sequences.DepositorInsertHuman Ensconsin (MAP7 Human, Synthetic)
UseExpression of a fluorescent microtubule markerTags3xEGFPExpressionMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRK6-HA-TAK1
Plasmid#44160DepositorInsertTak1 (Map3k7 Mouse)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceOct. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
FH1-PNGT#1-mCherry_PGK-H2B-CFP
Plasmid#164103PurposeFluorescent reporter for genetic tracing of proneural Glioblastoma cell-state.DepositorInsertPNGT#1-mCherry
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIRES:mTREK-1(G137I)
Plasmid#133274PurposeMammalian expression vector. It will generate the mouse TREK-1 channel full lenght G137I mutant fused to a N-terminal HA tagDepositorInsertPotassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)
UseTagsHAExpressionMammalianMutationG137IPromoterCMVd2Available sinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLex 983-Micu2
Plasmid#50372PurposeExpression in mammalian cellsDepositorInsertMICU2 (MICU2 Human)
UseLentivirusTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HLA-cMyc-EcopT1R1
Plasmid#113962Purposemammalian expression plasmid for c-myc-tagged E. coli codon-optimized human T1R1 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chainDepositorInsertT1R1 (TAS1R1 Human)
UseTagsSignal/leader sequence from HLA class I histocomp…ExpressionMammalianMutationtruncate N-terminal 24 residuesPromoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIDK-HDAC1fl
Plasmid#109158PurposeEncodes human full-length HDAC1 to be expressed in a baculovirus/insect cell expression systemDepositorInserthuman full-length HDAC1, sequence-optimized for insect cells (HDAC1 Human)
UseTagsExpressionInsectMutationPromoterp10Available sinceJan. 11, 2019AvailabilityAcademic Institutions and Nonprofits only