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Plasmid#68435PurposeTransient expression of the "TOP2" construct, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.DepositorInsertTOP2 construct
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
KA2601_eSpCas9-G2P
Plasmid#124203PurposeExpression of eSpCas9(1.1) and sgRNADepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTd-4-15-13-10
Plasmid#63835PurposeThis plasmid is designed to validate the biophysical model of translational couplingDepositorInsertCFP, mRFP1 and GFPmut3b
UseTagsExpressionBacterialMutationThe intergenic distance between each gene is -4 n…PromoterJ23100Available sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCherry-eDHFR-SIMvada
Plasmid#164649PurposeSIM mutant tagged with mCherry and eDHFRDepositorInsertSIMvada
UseCre/LoxTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pU6_(GLuc)_INT[Spinach2]
Plasmid#68430PurposeTransient expression in mammalian cells of an "INT" construct_bearing the "Spinach2" aptamer, targeting the GLuc reporter. U6 promoterDepositorInsertINT construct_bearing the "Spinach2" aptamer
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_ygiF-CHAD
Plasmid#127793PurposeExpresses the CHAD domain of ygiF in E. coli cellsDepositorInsertygiF-CHAD (ygiF Escherichia coli)
UseTags6xHis tag + TEV cleavage siteExpressionBacterialMutationN-terminal TTM domain of ygiF (residues 201-433)PromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 CrkII
Plasmid#50731Purposemammalian expression of CrkIIDepositorInsertCrkII (CRK Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-dpcoCas9-TV
Plasmid#158409PurposeCRISPR-dpcoCas9 fused with TV activator for transcriptional activationDepositorInsertdpcoCas9-TV(6xTAL-2xVP64)
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSL0373 (His-MBP-TniQ)
Plasmid#130638PurposeExpresses V. cholerae CAST TniQ from a T7 promoter, with N-terminal His10-MBP-TEVsite tag for purification.DepositorInsertVchTniQ
UseCRISPR; TransposonTagsHis10-MBP-TEVsite on TniQExpressionBacterialMutationPromoterT7Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
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Lentiviral FLAG-CaMKKbeta
Plasmid#32458DepositorInsertCalcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)
UseLentiviralTagsFLAGExpressionMutationPromoterAvailable sinceFeb. 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
pU6_(GLuc)_TOP1
Plasmid#68423PurposeTransient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U6 promoterDepositorInsertTOP1 construct
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQE60 Trx1 CCAAA
Plasmid#98402PurposeBacterial expression vector for Trx1 CCAAADepositorInsertTrx1 CCAAA (TXN Human)
UseTagsExpressionBacterialMutationC62A, C69A, C73APromoterAvailable sinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQE60 Trx1 SSAAA
Plasmid#98403PurposeBacterial expression vector for Trx1 SSAAADepositorInsertTrx1 SSAAA (TXN Human)
UseTagsExpressionBacterialMutationC32S, C35S, C62A, C69A, C73APromoterAvailable sinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV/MASC_(GLuc)_INT
Plasmid#68440PurposeTransient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. CMV/ MASC expression backbone.DepositorInsertINT construct_bearing three PP7 Stem-loops
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
KA2058_pX330A-G2P
Plasmid#124202PurposeExpression of SpCas9 and sgRNADepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pISH-Emx2
Plasmid#74346PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Emx2. Not for gene expression.DepositorInsertempty spiracles homeobox 2 (Emx2 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-T-V5-GR-2A-H2BmCherry-IP
Plasmid#171500PurposeOverexpression of T(Brachyury)-GRDepositorInsertT
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDC146
Plasmid#169366PurposeExpresses Syntaxin1-265 C145S, S249C, K253C ; SNAP25 ; 6xHis-TEV-Synaptobrevin1-96DepositorInsertSyntaxin1-265 C145S, S249C, K253C ; SNAP25 ; 6xHis-TEV-Synaptobrevin1-96
UseTags6 his tagExpressionBacterialMutationPromoterT7Available sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRK-flag-GFP 1-10 tetra Cys
Plasmid#78589PurposeExpression flag-GFP 1-10 tetra Cys in mammalian cellsDepositorInsertflag-GFP 1-10 tetra Cys
UseTagsflagExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only